Immobilization of enzymes on PTFE surfaces

Keusgen, Michael; Glodek, Janina; Milka, Peter; Krest, Ingo

doi:10.1002/1097-0290(20010305)72:5<530::aid-bit1017>3.0.co;2-j

Cited by 21 publications

(3 citation statements)

References 19 publications

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“…Miyagawa et al prepared glycosylated cellulose membranes by immobilizing the glycoconjugate polymer on carboxymethylated membranes through condensation reaction between the amino group of the glycoconjugates and the carboxyl group of the cellulose. Keusgen and coworkers pretreated polytetrafluoroethylene (PTFE) membrane with elementary sodium followed by oxidation using ozone or hydrogen peroxide, and then mannose ligands were immobilized on the membrane surface through coupling reagent, 1,4‐butanediol diglycidyl ether. Tabary et al modified PVDF membranes by impregnating them with the reactants of saccharide derivate (cycldextrin, maltodextrin, and citric acid) in a thermofixation oven.…”

Section: Glycosylation Methodsmentioning

confidence: 99%

“…It has been reported that β(1 → 4) galactosyltransferase expressed as a fusion protein with binding MBP‐galactosyltransferase was displayed specifically on a Langmuir–Blodgett membrane through maltotriose‐MBP interaction . Alliinase was also immobilized on PTFE membrane surface indirectly by a saccharide‐lectin binding . The saccharide mannan was bound to the membrane surface as an anchor for layers of Con A.…”

Section: Applications Of the Glycosylated Membranesmentioning

confidence: 99%

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Glycosylated membranes: A promising biomimetic material

Fang

2013

J of Applied Polymer Sci

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Development in the area of glycosylated membranes has been actively pursued in the past few years. This kind of promising biomimetic material is inspired by cell membranes. The recent surge of interest in these glycosylated membranes stems from their widespread number of applications to many areas in science and technology. With the glycosylation strategy, membrane separation properties, such as flux and antifouling, are greatly improved. Moreover, the ability to modulate biocompatibility, protein recognition, separation of biomolecules, enzyme immobilization, cell culture, and microorganisms capture are important in a variety of biological and medical applications. This review focuses on the recent progress in the preparation of these glycosylated membranes and highlights their applications.

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Section: Glycosylation Methodsmentioning

confidence: 99%

Section: Applications Of the Glycosylated Membranesmentioning

confidence: 99%

Glycosylated membranes: A promising biomimetic material

Fang

2013

J of Applied Polymer Sci

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“…As we showed in a previous study, APTES-coated glass substrates are functional up to 3 months of storage at room temperature or 4 °C, after which hydrolysis significantly reduces the receptor immobilization efficiency. Similarly, thiols such as 11-mercaptoundecanoic acid (MUA) have been used to generate COOH functional groups on gold-based substrates. − On the other hand, acid/base treatments and highly reactive ionized gas plasmas have been used to generate NH 2 or COOH functional groups on thermoplastic substrates such as poly(methyl methacrylate) (PMMA), , polytetrafluoroethylene (PTFE), , and polycarbonate (PC) . Using covalent immobilization strategies, typical desirable b max values ranging from 10 –9 to 10 –7 mol/m 2 can be obtained, , whose values mimic the distribution of receptors on surfaces of cells …”

Section: Enhancing Analytical Sensitivity and Specificitymentioning

confidence: 99%

Toward the Development of Rapid, Specific, and Sensitive Microfluidic Sensors: A Comprehensive Device Blueprint

Sathish

Shen

2021

JACS Au

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Recent advances in nano/microfluidics have led to the miniaturization of surface-based chemical and biochemical sensors, with applications ranging from environmental monitoring to disease diagnostics. These systems rely on the detection of analytes flowing in a liquid sample, by exploiting their innate nature to react with specific receptors immobilized on the microchannel walls. The efficiency of these systems is defined by the cumulative effect of analyte detection speed, sensitivity, and specificity. In this perspective, we provide a fresh outlook on the use of important parameters obtained from well-characterized analytical models, by connecting the mass transport and reaction limits with the experimentally attainable limits of analyte detection efficiency. Specifically, we breakdown when and how the operational (e.g., flow rates, channel geometries, mode of detection, etc.) and molecular (e.g., receptor affinity and functionality) variables can be tailored to enhance the analyte detection time, analytical specificity, and sensitivity of the system (i.e., limit of detection). Finally, we present a simple yet cohesive blueprint for the development of high-efficiency surface-based microfluidic sensors for rapid, sensitive, and specific detection of chemical and biochemical analytes, pertinent to a variety of applications.

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Enzyme immobilization on ultrafine cellulose fibers via poly(acrylic acid) electrolyte grafts

Chen

Hsieh

2005

Biotechnol. Bioeng.

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Ultrafine cellulose fiber (diameter 200-400 nm) surfaces were grafted with polyacrylic acid (PAA) via either ceric ion initiated polymerization or methacrylation of cellulose with methacrylate chloride (MACl) and subsequent free-radical polymerization of acrylic acid. PAA grafts by ceric ion initiated polymerization increased with increasing reaction time (2-24 h), monomer (0.3-2.4 M), and initiator (1-10 mM) concentrations, and spanned a broad range from 5.5-850%. PAA grafts on the methacrylated cellulose fibers also increased with increasing molar ratios of MACl to cellulosic hydroxyl groups (MACl/OH, 2-6.4) and monomer acrylic acid (AA) to initiator potassium persulfate (KPS) ratios ([AA]/[KPS], 1.5-6), and were in a much narrower range between 12.8% and 29.4%. The adsorption of lipase (at 1 mg/ml lipase and pH 7) and the activity of adsorbed lipase (pH 8.5, 30 degrees C), in both cases decreased with increasing PAA grafts. The highest adsorption and activity of the lipase on the ceric ion initiated grafted fibers were 1.28 g/g PAA and 4.3 U/mg lipase, respectively, at the lowest grafting level of 5.5% PAA, whereas they were 0.33 g/g PAA and 7.1 U/mg lipase, respectively, at 12.8% PAA grafts on the methacrylated and grafted fibers. The properties of the grafted fibers and the absorption behavior and activity of lipase suggest that the PAA grafts are gel-like by ceric-initiated reaction and brush-like by methacrylation and polymerization. The adsorbed lipase on the ceric ion-initiated grafted surface possessed greatly improved organic solvent stability over the crude lipase. The adsorbed lipases exhibited 0.5 and 0.3 of the initial activity in the second and third assay cycles, respectively.

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Immobilization of enzymes on PTFE surfaces

Cited by 21 publications

References 19 publications

Glycosylated membranes: A promising biomimetic material

Glycosylated membranes: A promising biomimetic material

Toward the Development of Rapid, Specific, and Sensitive Microfluidic Sensors: A Comprehensive Device Blueprint

Enzyme immobilization on ultrafine cellulose fibers via poly(acrylic acid) electrolyte grafts

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