Immobilization of Glucose Oxidase on Polydopamine-Functionalized Graphene Oxide

Zhou, Liya; Jiang, Yanjun; Ma, Li; Gao, Jing

doi:10.1007/s12010-014-1324-1

Cited by 36 publications

(18 citation statements)

References 38 publications

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“…The 1620 and 1740 cm −1 peaks could arise from changes of the stretching vibration of C=C and the stretching vibration of conjugated carbonyl groups (-C=O) or of carboxyl groups (–COOH) on the edges of the basal planes, respectively. These latter peaks also suggest potential polymerization of DA on the graphene surface [50]. …”

Section: Resultsmentioning

confidence: 99%

Raman and Conductivity Analysis of Graphene for Biomedical Applications

et al. 2016

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In this study, we present a comprehensive investigation of graphene’s optical and conductive properties using confocal Raman and a Drude model. A comparative analysis between experimental findings and theoretical predictions of the material’s changes and improvements as it transitioned from three-dimensional graphite is also presented and discussed. Besides spectral recording by Raman, which reveals whether there is a single, a few, or multi-layers of graphene, the confocal Raman mapping allows for distinction of such domains and a direct visualization of material inhomogeneity. Drude model employment in the analysis of the far-infrared transmittance measurements demonstrates a distinct increase of the material’s conductivity with dimensionality reduction. Other particularly important material characteristics, including carrier concentration and time constant, were also determined using this model and presented here. Furthermore, the detection of micromolar concentration of dopamine on graphene surfaces not only proves that the Raman technique facilitates ultrasensitive chemical detection of analytes, besides offering high information content about the biomaterial under study, but also that carbon-based materials are biocompatible and favorable micro-environments for such detection. Such information is valuable for the development of bio-medical sensors, which is the main application envisioned for this analysis.

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Section: Resultsmentioning

confidence: 99%

Raman and Conductivity Analysis of Graphene for Biomedical Applications

et al. 2016

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“…Many attempts have been performed to increase enzyme stability with the help of continuous reactant concentration (i.e. glucose), enzyme immobilization or enhanced reaction area by 3D structure [43][44][45] , which will be utilized further for enhancing or prolonging biological functions of EBFC. Within the limitation of the above short-term stability of generating electrical cues, EBFCs were applied for culturing cells in media for up to 24 hrs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Performance of a glucose-reactive enzyme-based biofuel cell system for biomedical applications

Jeon

Lee

Dashnyam

et al. 2019

Sci Rep

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A glucose-reactive enzyme-based biofuel cell system (EBFC) was recently introduced in the scientific community for biomedical applications, such as implantable artificial organs and biosensors for drug delivery. Upon direct contact with tissues or organs, an implanted EBFC can exert effects that damage or stimulate intact tissue due to its byproducts or generated electrical cues, which have not been investigated in detail. Here, we perform a fundamental cell culture study using a glucose dehydrogenase (GDH) as an anode enzyme and bilirubin oxidase (BOD) as a cathode enzyme. The fabricated EBFC had power densities of 15.26 to 38.33 nW/cm 2 depending on the enzyme concentration in media supplemented with 25 mM glucose. Despite the low power density, the GDH-based EBFC showed increases in cell viability (~150%) and cell migration (~90%) with a relatively low inflammatory response. However, glucose oxidase (GOD), which has been used as an EBFC anode enzyme, revealed extreme cytotoxicity (~10%) due to the lethal concentration of H 2 o 2 byproducts (~1500 µM). Therefore, with its cytocompatibility and cell-stimulating effects, the GDH-based EBFC is considered a promising implantable tool for generating electricity for biomedical applications. Finally, the GDH-based EBFC can be used for introducing electricity during cell culture and the fabrication of organs on a chip and a power source for implantable devices such as biosensors, biopatches, and artificial organs.

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“…For a biohybrid motor with a “kill” trigger, the two‐layer biohybrid had urease immobilized on the PDA. PDA contains catechol moieties capable of covalently binding amine groups from enzymes allowing Ppy–PDA microtubes to be functionalized with urease by a simple incubation and rinsing procedure. E. coli attached and swam with the urease modified tubes without hindrance (Figure B).…”

Section: Resultsmentioning

confidence: 99%

Biohybrid Microtube Swimmers Driven by Single Captured Bacteria

Stanton

Park

Miguel-López

et al. 2017

Small

145

115

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Bacteria biohybrids employ the motility and power of swimming bacteria to carry and maneuver microscale particles. They have the potential to perform microdrug and cargo delivery in vivo, but have been limited by poor design, reduced swimming capabilities, and impeded functionality. To address these challenge, motile Escherichia coli are captured inside electropolymerized microtubes, exhibiting the first report of a bacteria microswimmer that does not utilize a spherical particle chassis. Single bacterium becomes partially trapped within the tube and becomes a bioengine to push the microtube though biological media. Microtubes are modified with “smart” material properties for motion control, including a bacteria‐attractant polydopamine inner layer, addition of magnetic components for external guidance, and a biochemical kill trigger to cease bacterium swimming on demand. Swimming dynamics of the bacteria biohybrid are quantified by comparing “length of protrusion” of bacteria from the microtubes with respect to changes in angular autocorrelation and swimmer mean squared displacement. The multifunctional microtubular swimmers present a new generation of biocompatible micromotors toward future microbiorobots and minimally invasive medical applications.

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Immobilization of Glucose Oxidase on Polydopamine-Functionalized Graphene Oxide

Cited by 36 publications

References 38 publications

Raman and Conductivity Analysis of Graphene for Biomedical Applications

Raman and Conductivity Analysis of Graphene for Biomedical Applications

Performance of a glucose-reactive enzyme-based biofuel cell system for biomedical applications

Biohybrid Microtube Swimmers Driven by Single Captured Bacteria

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