Immobilization of Growing Cells and Its Application to the Continuous Ethanol Fermentation Process

Joung, John J.; Royer, Garfield P.

doi:10.1111/j.1749-6632.1990.tb24252.x

Cited by 7 publications

(1 citation statement)

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“…A similar disparity between theoretical and experimental data was reported recently by Young and Royer (1990) for growing yeast cells immobilised in an expandable polyethyleneimine alginate matrix; the experimental values were substantially lower than the theoretical values when the substrate saturation parameter reached 100.…”

Section: Convective Transport: Neglectedsupporting

confidence: 83%

Theoretical Studies on Immobilised Cell Fermentation Technology

Δερβακοσ¹

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A critical examination is made of the many experimental and theoretical studies relating to the use of immobilised metabolically-intact whole cells to carry out biological reactions -a technique which can be defined as Immobilised Cell Fermentation Technology (ICFT).The first part of the work was mainly designed to consolidate the published work in the form of a computer package, including database and mathematical modules. Application of the package has shown that while most of the advantages which are often claimed for the technology have been verified experimentally, there is still much scope for revising the form of the standard diffusion-reaction equations which are ordinarily applied to describe the behaviour of these systems. It was illustrated that application of such models may lead to disparities between theoretical and empirical data. The observed differences were also discussed in the light of experimental limitations.This study illustrates the need to take into account the particular characteristics of immobilised viable cells when modelling their behaviour. George A. Dervakos Ph.D Thesis UMIST 1990 Declaration None of the material contained in this Thesis has been submitted in support of an application for another degree or qualification of this or any other university or other institute of learning. George A. Dervakos 3.2.6 The increased product stability 57 3.2.7 The integration with downstream processing 58 3.2.8 Advantages due to cell proximity 59 3.2.9 The increase in reaction selectivity 3.2.10 The versatility in the selection of the reactor 60 The metabolic changes in immobilised cells 61 Problems introduced by cell immobilisation 68 3.4.1 Mechanical problems 69 3.4.2 Increased substrate limitations 73 3.4.3 Increased product inhibition 74 Conclusions APPLICATION OF THE DESIGN MODULE: A PRELIMINARY LOOK AT INTRA-PARTICLE DIFFUSION LIMITATIONS IN IMMOBILISED CELL SYSTEMS Introduction Ethanol production with immobilised cells 79 Oxygen transport in immobilised cell aggregates Cellulase production using cells immobilised in biomass support particles . . 94 Conclusions FURTHER EXAMINATION OF EFFECTIVE DD7FUSIVITIES .... Introduction The structure of the immobilised cell aggregate 5.2.1 The volume fraction of cells within the aggregate 5.2.2 The volume fraction of cell clusters within the aggregate 5.2.3 The volume fraction of carrier within the aggregate 5.2.4 Some idiosyncrasies of the structure of the immobilised cell aggregate Diffusive flux in the immobilised cell aggregate 5.3.1 The definition of the effective diffusivity 5.3.2 Experimental work xii 5.4 The errors and misunderstandings 118 5.5 Summary 128 CHAPTER 6: FURTHER EXAMINATION OF DIFFUSION/REACTION THEORY . 6.1Table 6.4: Parametric values used in the dynamic simulation in Fig 6.37 (linear inhibition)

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Section: Convective Transport: Neglectedsupporting

confidence: 83%