Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants

Abstract

Removing organic pollutants from wastewater is crucial to prevent environmental contamination and protect human health. Immobilized enzymes are increasingly being explored for wastewater treatment due to their specific catalytic activities, reusability, and stability under various environmental conditions. Peroxidases, such as horseradish peroxidase (HRP) and myoglobin (Mb), are promising candidates for immobilized enzymes utilized in wastewater treatment due to their ability to facilitate the oxidation process of a wide range of organic molecules. However, the properties of the carrier and support materials greatly influence the stability and activity of immobilized HRP and Mb. In this research, we developed immobilized HRP and Mb using support material composed of sodium alginate and CaCl2 as carriers and glutaraldehyde as a crosslinking agent. Following this, the efficacy of immobilized HRP and Mb in removing aniline, phenol, and p-nitrophenol was assessed. Both immobilized enzymes removed all three organic pollutants from an aqueous solution, but Mb was more effective than HRP. After being immobilized, both enzymes became more resilient to changes in temperature and pH. Both immobilized enzymes retained their ability to eliminate organic pollutants through eight treatment cycles. Our study uncovered novel immobilized enzyme microspheres and demonstrated their successful application in wastewater treatment, paving the way for future research.

Metal/Covalent Organic Framework Encapsulated Lead-Free Halide Perovskite Hybrid Nanocatalysts: Multifunctional Applications, Design, Recent Trends, Challenges, and Prospects

“…The gel obtained is dried and then calcined at high temperatures to get the required perovskite material. 25 One more solution-based technique is the precipitation method, which requires the controlled precipitation of metal ions from a solution consisting of suitable precursors. Usually, two separate solutions containing the A-site and B-site precursors are prepared and mixed under controlled conditions.…”

“…The sol is then subjected to a gelation process, which can be achieved through various techniques, such as hydrolysis or condensation reactions. The gel obtained is dried and then calcined at high temperatures to get the required perovskite material . One more solution-based technique is the precipitation method, which requires the controlled precipitation of metal ions from a solution consisting of suitable precursors.…”