Immobilization of horseradish peroxidase on modified PAN‐based membranes for the removal of phenol from buffer solutions

Abstract

Polyacrylonitrile ultrafiltration membranes have been widely used in many separation processes. In this paper, horseradish peroxidase was immobilized onto polyacrylonitrile ultrafiltration membrane by crosslinking with glutaraldehyde. The immobilized enzyme possessed a protein loading of 0.025 mg/cm2membrane and a specific activity of 105 U/mgprotein (105 μmol/min/mgprotein). The initial modified and immobilized membranes were observed using SEM and FTIR. Potential applications of HRP membranes were investigated in the removal of phenol through oxidation with the addition of hydrogen peroxide. The optimum pH of the immobilized enzyme was determined to be 6.0, and the optimum hydrogen peroxide concentration to be 30 mmol/L. Almost 100 % removal of phenol (1 ∼ 10 mg/L) from water was achieved by HRP membranes. For high concentrated solutions, successive cycles were successfully used to improve the degree of phenol oxidation. Furthermore, the immobilized horseradish peroxidase was operationally stable. These results suggest that the HRP membrane has promising applications for the removal of phenol.

Immobilization of Peroxidase on Functionalized MWCNTs-Buckypaper/Polyvinyl alcohol Nanocomposite Membrane

“…The presence of these acylamino groups (combination of –CHO and –NH 2 ) indicates amidination reaction and confirmed the immobilization of JP on the BP/PVA membrane 59 . This statement is further supported by the previously reported studies 60–62 . Additionally, the crosslinks between functionalized BP/PVA membrane and glutaraldehyde are detected with an increase in the intensity of C-H band at 2850–3270 cm −1 42 .…”

Development of chia gum/alginate-polymer support for horseradish peroxidase immobilization and its application in phenolic removal

“…In addition, the amide groups of the HRP including amide I (C=O stretching), amide II (N-H deformation), and amide III (C-N stretching and N-H bending) are also absorbed/appeared at 1600, 1429, 1016 cm −1 , respectively, and C-H bond bending or deformation demonstrated at 600 cm 1 . The appearance of these groups indicates an amidation reaction and confirms the immobilization of HRP on the ACG support [31][32][33] . Further, the bands/ peaks at 2924, 2848, 1650, 1449, 1298, and 964 cm −1 are shifted to 2918, 2842, 1600, 1429, 1305, and 1016 cm −1 , respectively.…”

Multi-faceted strategy based on enzyme immobilization with reactant adsorption and membrane technology for biocatalytic removal of pollutants: A critical review

“…Nevertheless, the main mechanism of removal was enzymatic biodegradation, and ultimately less than 10% of the phenol was removed by sorption. With the use of the described system, almost total removal of phenol from water solutions at concentrations up to 10 mg/L was achieved, which suggests that a polyacrylonitrile membrane with immobilized horseradish peroxidase has promising applications for the removal of phenol from water solutions (Wang et al, 2016).…”