Immobilization of horseradish peroxidase onto Purolite® <scp>A109</scp> and its anthraquinone dye biodegradation and detoxification potential

Šekuljica, Nataša Ž.; Jovanović, Jelena; Tanasković, Sonja Jakovetić; Ognjanović, Nevena D.; Gazikalović, Ivana; Knežević‐Jugović, Zorica; Mijin, Dušan Ž.

doi:10.1002/btpr.2991

Cited by 17 publications

(8 citation statements)

References 37 publications

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“…The maximum capacity of HRP adsorption on kaolin has been found to be 4.63 mg/g [53]. However, when comparing the activity of HRP-MAB with values achieved in the literature for the same type of HRP, similar or better results have been described by several researchers, ranging from ~5 to ~85 U/g [13,53]. Steric problems frequently associated with high loadings on the carrier are a possible explanation for discrepancy from some expected values.…”

Section: Effect Of Immobilization Conditions On Enzymatic Activity An...mentioning

confidence: 50%

“…These results are similar to or favorable compared with those reported in the literature for HRP immobilized on other carriers. For the purpose of comparison, a maximum HRP binding of 4.8 mg/g and 3.3 mg/g has been achieved on a commercial resin (Purolite®A109) by adsorption and covalent immobilization, respectively [13]. The maximum capacity of HRP adsorption on kaolin has been found to be 4.63 mg/g [53].…”

Section: Effect Of Immobilization Conditions On Enzymatic Activity An...mentioning

confidence: 99%

“…Bioremediation of synthetic dye wastewater is the latest approach in addressing this environmental problem. It is classified as a completely natural method where organic dyes are converted into completely harmless end products [13].…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation

et al. 2022

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The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.

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Section: Effect Of Immobilization Conditions On Enzymatic Activity An...mentioning

confidence: 50%

Section: Effect Of Immobilization Conditions On Enzymatic Activity An...mentioning

confidence: 99%

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Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation

et al. 2022

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“…After the detailed examination of differential genes and pathway expression, the genes involved in the possible mechanism of enhanced dye removal performance by FG were generally divided into three groups. The first group was extracellular non-specific peroxidase, including LiP, MnP, Lac, and DyP, which have been considered as the main participants in the biodegradation process of anthraquinone synthetic dyes ( Hsu et al, 2012 ; Uchida et al, 2015 ; Navada et al, 2018 ; Sekuljica et al, 2020 ). Previous research has tended to focus on a single enzyme involved in the dye biodegradation process.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Reactive Blue 4 Biodegradation Performance of Newly Isolated white rot fungus Antrodia P5 by the Synergistic Effect of Herbal Extraction Residue

et al. 2021

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In this study, a white rot fungus Antrodia was newly isolated and named P5. Then its dye biodegradation ability was investigated. Our results showed that P5 could effectively degrade 1,000 mg/L Reactive Blue 4 (RB4) in 24 h with 95% decolorization under shaking conditions. It could tolerate a high dye concentration of 2,500 mg/L as well as 10% salt concentration and a wide range of pH values (4–9). Herbal extraction residues (HER) were screened as additional medium elements for P5 biodegradation. Following the addition of Fructus Gardeniae (FG) extraction residue, the biodegradation performance of P5 was significantly enhanced, achieving 92% decolorization in 12 h. Transcriptome analysis showed that the expression of multiple peroxidase genes was simultaneously increased: Lignin Peroxidase, Manganese Peroxidase, Laccase, and Dye Decolorization Peroxidase. The maximum increase in Lignin Peroxidase reached 10.22-fold in the presence of FG. The results of UV scanning and LC-HRMS showed that with the synergistic effect of FG, P5 could remarkably accelerate the biodegradation process of RB4 intermediates. Moreover, the fungal treatment with FG also promoted the abatement of RB4 toxicity. In sum, white rot fungus and herbal extraction residue were combined and used in the treatment of anthraquinone dye. This could be applied in practical contexts to realize an efficient and eco-friendly strategy for industrial dye wastewater treatment.

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“…Peroxidases are another group of enzymes that can catalyze oxidation reactions. Peroxidases immobilized on various kinds of supports have been successfully utilized for the decolorization and detoxification of waste dyes [23,[58][59][60]. Many researchers have also used horseradish peroxide (HRP) in the degradation of organic dyes.…”

Section: Organic Dyesmentioning

confidence: 99%

Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment

Kołodziejczak-Radzimska

Nghiem

Jesionowski

2021

Curr Pollution Rep

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Purpose of Review Untreated wastewater discharge can significantly and negatively impact the state of the environment. Rapid industrialization and economic development have directly contributed to land and water pollution resulting from the application of many chemicals such as organic dyes, pharmaceuticals, and industrial reagents. The removal of these chemicals before effluent discharge is crucial for environmental protection. This review aims to explore the importance of functionalized materials in the preparation of biocatalytic systems and consider their application in eliminating water pollutants. Recent Findings Wastewater treatment methods can be classified into three groups: (i) chemical (e.g., chemical oxidation and ozonation), (ii) physical (e.g., membrane separation and ion exchange), and (iii) biological processes. Biological treatment is the most widely used method due to its cost-effectiveness and eco-friendliness. In particular, the use of immobilized enzymes has recently become more attractive as a result of scientific progress in advanced material synthesis. The selection of an appropriate support plays an important role in the preparation of such biologically active systems. Recent studies have demonstrated the use of various materials for enzyme immobilization in the purification of water. Summary This review identifies and discusses different biocatalytic systems used in the enzymatic degradation of various water pollutants. Materials functionalized by specific groups can serve as good support matrices for enzyme immobilization, providing chemical and thermal stability to support catalytic reactions. Enzymatic biocatalysis converts the pollutants into simpler products, which are usually less toxic than their parents. Due to immobilization, the enzyme can be used over multiple cycles to reduce the cost of wastewater treatment. Future studies in this field should focus on developing new platforms for enzyme immobilization in order to improve degradation efficiency.

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Immobilization of horseradish peroxidase onto Purolite® A109 and its anthraquinone dye biodegradation and detoxification potential

Cited by 17 publications

References 37 publications

Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation

Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation

Enhanced Reactive Blue 4 Biodegradation Performance of Newly Isolated white rot fungus Antrodia P5 by the Synergistic Effect of Herbal Extraction Residue

Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment

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