Immobilization ofAspergillus nigerF7-02 Lipase in Polysaccharide Hydrogel Beads ofIrvingia gabonensisMatrix

Abstract

The potential of polysaccharide Irvingia gabonensis matrix as enzyme immobilization support was investigated. Lipase of Aspergillus niger F7-02 was immobilized by entrapment using glutaraldehyde as the cross-linking agent and stabilized in ethanolic-formaldehyde solution. The pH and temperature stability and activity yield of the immobilized enzyme were determined. Such parameters as enzyme load, bead size, number of beads, and bead reusability were also optimized. Adequate gel strength to form stabilized beads was achieved at 15.52% (w/v) Irvingia gabonensis powder, 15% (v/v) partially purified lipase, 2.5% (v/v) glutaraldehyde, and 3 : 1 (v/v) ethanolic-formaldehyde solution. There was 3.93-fold purification when the crude enzyme was partially purified in two-step purification using Imarsil and activated charcoal. Optimum lipase activity 75.3 Ug−1 was achieved in 50 mL test solution containing 15 beads of 7 mm bead size. Relative activity 80% was retained at eight repeated cycles. The immobilization process gave activity yield of 59.1% with specific activity of 12.3 Umg−1 and stabilized at optimum pH 4.5 and temperature 55°C. Thus the effectiveness and cost-efficiency of I. gabonensis as a polymer matrix for lipase immobilization have been established.

Prospects of Biocatalyst Purification Enroute Fermentation Processes

“…There are considerable ways of maintaining enzyme efficiency. Purification technique is a very powerful device has been arduously used to make some economically attainable and high performance enzymes with improved stability [11]. Enzyme purification is imperative for a full apprehension of the description and established process of enzymes.…”

“…Its thickening characteristics have been attributed to the presence of hydrocolloid property or gelling property [53]. Imarsil possesses quick and simple recovery approach more importantly in the clarification of microbial biocatalyst from fermentation broth [11]. Cherry and Fidants, [54] demonstrated the use of carboxymethyl cellulose, edible gum and tannic acid as precipitants and as well as organic solvents also poses the problem of product recovery.…”

Spray-dried immobilized lipase fromGeobacillussp. strain ARM in sago

“…Some of the techniques to entrap enzymes are gel or fiber entrapping and micro-encapsulation. Lipase has been immobilized via entrapment or encapsulation in κ -carrageenan and polysaccharide hydrogel beads in previous studies (Jegannathan, Seng & Ravindra, 2009; Kareem, Adio & Osho, 2014). A recent technique to encapsulate lipase is by synthesizing a protein-inorganic hybrid nanoflower system, in which the protein molecules form complexes with copper ions (Ge, Lei & Zare, 2012; Jiang et al, 2018; Kumar et al, 2018).…”