Immobilization of <i>Candida antarctica</i> lipase B on Polystyrene Nanoparticles

Miletić, Nemanja; Abetz, Volker; Ebert, K.; Loos, Katja

doi:10.1002/marc.200900497

Cited by 89 publications

(38 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Candida antarctica Lipase B (CALB) is a very versatile biocatalyst for polyester synthesis, working with various monomers and organic solvents under mild conditions [12,14,15,16,17,18,19,20,21,22,23,24,25,26,27]. CALB-catalyzed synthesis of polyesters from green monomers has recently gained increasing popularity.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-Catalyzed Synthesis of Unsaturated Aliphatic Polyesters Based on Green Monomers from Renewable Resources

et al. 2013

Self Cite

View full text Add to dashboard Cite

Bio-based commercially available succinate, itaconate and 1,4-butanediol are enzymatically co-polymerized in solution via a two-stage method, using Candida antarctica Lipase B (CALB, in immobilized form as Novozyme® 435) as the biocatalyst. The chemical structures of the obtained products, poly(butylene succinate) (PBS) and poly(butylene succinate-co-itaconate) (PBSI), are confirmed by 1H- and 13C-NMR. The effects of the reaction conditions on the CALB-catalyzed synthesis of PBSI are fully investigated, and the optimal polymerization conditions are obtained. With the established method, PBSI with tunable compositions and satisfying reaction yields is produced. The 1H-NMR results confirm that carbon-carbon double bonds are well preserved in PBSI. The differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA) results indicate that the amount of itaconate in the co-polyesters has no obvious effects on the glass-transition temperature and the thermal stability of PBS and PBSI, but has significant effects on the melting temperature.

show abstract

Section: Introductionmentioning

confidence: 99%

Enzyme-Catalyzed Synthesis of Unsaturated Aliphatic Polyesters Based on Green Monomers from Renewable Resources

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Miletic et al [47] also reported that immobilization of enzyme on polystyrene nanoparticles resulted in their increased activity. Thus, enhanced stabilization of nitrogenase at low concentrations of nano-ZnO could be one possible mechanism to explain the observed enhanced activity.…”

Section: Interplay Of Concentration and Timementioning

confidence: 97%

Effect of nano-zinc oxide on nitrogenase activity in legumes: an interplay of concentration and exposure time

2015

View full text Add to dashboard Cite

Experiments were carried out to study the effect of zinc oxide nanoparticles (nano-ZnO) on nitrogenase activity in legumes. In the first experiment, nodulated roots of cluster bean, moth bean, green gram and cowpea were dipped in Hoagland solution containing 1.5 and 10 lg mL -1 of nano-ZnO for 24 h. Nitrogenase activity in cluster bean, green gram and cowpea roots increased after dipping in solution containing 1.5 lg mL -1 nano-ZnO, but decreased in roots dipped in solution containing 10 lg mL -1 nano-ZnO. However, in moth bean roots, nitrogenase activity decreased after dipping in solution containing either concentration of nano-ZnO. In the second experiment, nodulated roots of green gram were dipped in Hoagland solution containing 1, 4, 6, 8 and 10 lg mL -1 nano-ZnO for 6-30 h before estimating nitrogenase activity. Results showed that an interactive effect of nanoZnO concentration and exposure time influenced nitrogenase activity. The possible reasons behind this effect have been discussed. A model [A = 3.44 ? 0.46t -0.01t 2 -0.002tc 2 (R 2 = 0.81)] involving linear and power components was developed to simulate the response of nitrogenase activity in green gram roots to the concentration and exposure time of nano-ZnO.

show abstract

“…Thus, regeneration for reuse is often a difficult task and requires high-speed centrifugation for a long time. Non-magnetic nanoparticle carriers include zirconia, silica, polystyrene, chitosan, and polylactic acid (Table 1) [39,[47][48][49][50][51]. When immobilized on a hydrophilic inorganic surface, lipases have poor binding ability because a polypeptide chain "lid" of lipases secludes the catalytic site [52].…”

Section: Non-magnetic Nanoparticlesmentioning

confidence: 99%

“…The relative activity of the lipase immobilized on the EDA-GA and EDA-NCS silica carriers was 115% and 107% compared to free enzyme, respectively. Candida antarctica lipase B (CAL-B) was immobilized on polystyrene nanoparticles via adsorption [49]. Polystyrene nanoparticles were synthesized using a nanoprecipitation technique.…”

Section: Non-magnetic Nanoparticlesmentioning

confidence: 99%

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Kim

Lee

2018

Catalysts

View full text Add to dashboard Cite

Abstract:The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. Recently, enzymes have been integrated with nanomaterials as immobilization carriers. Nanomaterials can increase biocatalytic efficiency. The development of a nano-immobilized enzyme is one of the key factors for cost-effective biodiesel production. This paper presents the technology development of nanomaterials, including nanoparticles (magnetic and non-magnetic), carbon nanotubes, and nanofibers, and their application to the nano-immobilization of biocatalysts. The current status of biodiesel production using a variety of nano-immobilized lipase is also discussed.

show abstract

Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

Cited by 89 publications

References 35 publications

Enzyme-Catalyzed Synthesis of Unsaturated Aliphatic Polyesters Based on Green Monomers from Renewable Resources

Enzyme-Catalyzed Synthesis of Unsaturated Aliphatic Polyesters Based on Green Monomers from Renewable Resources

Effect of nano-zinc oxide on nitrogenase activity in legumes: an interplay of concentration and exposure time

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Contact Info

Product

Resources

About