Immobilization of invertase on chitosan and its application to honey treatment

Nam, Nguyen Xuan; Nghia, Ho Trung Trong; Vy, Le Thi Tuong; Oanh, Huỳnh Ngọc; Hien, Phan Phuoc

doi:10.1063/1.5000173

Cited by 1 publication

(1 citation statement)

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“…Similar results were obtained by Lorenzoni et al (2014Lorenzoni et al ( , 2015, with chitosan particles activated with GA, that lead to not only higher thermal stability but also a retention of high enzymatic activity after 50 cycles of use in a batch production of FOS. For immobilization in chitosan, Nam et al (2017) proposed that the occurrence of optimum pH and temperature shifts, when compared with the FE may occur due to alterations of the physical and chemical properties of the enzyme during the immobilization process.…”

Section: Support Materials and Approaches For Enzymatic Production Ofmentioning

confidence: 99%

Recent advances in β-galactosidase and fructosyltransferase immobilization technology

Ureta

Martins

Figueira

et al. 2020

Critical Reviews in Food Science and Nutrition

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The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal of enzymes for their reusability. The aim of this review is to go through the principal immobilization strategies addressed to achieve optimal industrial processes with special care on those reported for two types of enzymes: b-galactosidases and fructosyltransferases. The main methods used to immobilize these two enzymes are adsorption, entrapment, covalent coupling and cross-linking or aggregation (no support is used), all of them having pros and cons. Regarding the support, it should be cost-effective, assure the reusability and an easy recovery of the enzyme, increasing its stability and durability. The discussion provided showed that the type of enzyme, its origin, its purity, together with the type of immobilization method and the support will affect the performance during the enzymatic synthesis. Enzymes' immobilization involves interdisciplinary knowledge including enzymology, nanotechnology, molecular dynamics, cellular physiology and process design. The increasing availability of facilities has opened a variety of possibilities to define strategies to optimize the activity and re-usability of b-galactosidases and fructosyltransferases, but there is still great place for innovative developments.

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Section: Support Materials and Approaches For Enzymatic Production Ofmentioning

confidence: 99%