Immobilization of invertase on chitosan coated γ-Fe 2 O 3 magnetic nanoparticles to facilitate magnetic separation

Waifalkar, P. P.; Parit, S.B.; Chougale, Ashok D.; Sahoo, Subasa C.; Patil, Pramod S.; Patil, Prasad

doi:10.1016/j.jcis.2016.07.082

Cited by 70 publications

(20 citation statements)

References 39 publications

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“…The procedure shown in this work is fast and simple and requires no harmful chemicals as previously described [2,19], despite the inclusion of a sonication process. Nanobiocatalysts combining enzymes and nanocarriers are drawing increased attention because of their high catalytic performance, enhanced stability, improved enzyme-substrate affinity, and reusability [20][21][22]. Genipin is a heterocycle of natural origin used as a nontoxic chemical for cross-linking of proteins and polysaccharides [10].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Díaz-Hernández

Gracida

García-Almendárez

et al. 2018

Journal of Nanomaterials

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Cross-linking of magnetic nanoparticles with proteins plays a significant role in the preparation of new materials for biotechnological applications. The aim was the maximization of the magnetic mass attracted and protein loading of magnetic iron oxide nanoparticles coated with chitosan, synthesized in a single step by alkaline precipitation. Chitosan-coated magnetite particles (Fe3O4@Chitosan) were cross-linked to a xylanase and a cellulase (Fe3O4@Chitosan@Proteins), showing a 93% of the magnetic saturation of the magnetite. X-ray diffraction pattern in composites corresponds to magnetite. Thermogravimetry and differential scanning calorimetry showed that 162 mg of chitosan was coating one gram of composite and 12 mg of protein was cross-linked to each gram of magnetic support. Cross-linking between enzymes and Fe3O4@Chitosan was confirmed by infrared spectroscopy with Fourier transform, X-ray energy, and X-ray photoelectron spectroscopy dispersion analysis. From dynamic light scattering, transmission and electron microscopy the average particle size distribution was 230 nm and 430 nm for Fe3O4@Chitosan and Fe3O4@Chitosan@Proteins, showing agglomerates of individual spherical particles, with an average diameter of 8.5 nm and 10.8 nm, respectively. The preparation method plays a key role in determining the particle size and shape, size distribution, surface chemistry, and, therefore, the applications of the superparamagnetic nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Díaz-Hernández

Gracida

García-Almendárez

et al. 2018

Journal of Nanomaterials

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“…The free and immobilized invertase (EC 3.2.1.26) produces high quality invert sugar with low concentrations of 5-hydroxymethyl-2-furfural (HMF – a carcinogenic byproduct) and without color development compared to the colored version obtained through acid hydrolysis [3], [4]. Invertase is one of the most studied enzymes and it has been immobilized by different methods and supports [5], [6], [7], [8].…”

Section: Introductionmentioning

confidence: 99%

“…These composites under nanoparticles sizes add the advantage of high surface to volume ratio such as the immobilized invertase on chitosan coated γ-Fe 2 O 3 magnetic nanoparticles [7]. Diatomaceous earth (DE) or diatomite is a naturally occurring clay from geological deposits composed predominantly of the fossilized skeletons of diatoms.…”

Section: Introductionmentioning

confidence: 99%

High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles

Cabrera

Assis

Neri

et al. 2017

Biotechnology Reports

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“…Magnetic nanoparticles exhibit specific physical properties and are of great interest because of their prospective applications in biology and medicine [1][2][3][4][5] for magnetic cell separation [6], magnetically controlled delivery of anticancer drugs [7,8], magnetic resonance imaging (MRI) contrast enhancement [9,10], and hypothermia treatment [11]. Most of these applications require chemically stable, well-dispersed, and uniform sized particles.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Cobalt and Cobalt Oxide Nanoparticles in Hyperbranched Polyester Polyol Matrix

Медведева

Kambulova

Bondar

et al. 2017

Journal of Nanotechnology

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A series of cobalt (Co) and its oxides based nanoparticles were synthesized by using hyperbranched polyester polyol Boltorn H20 as a platform and sodium borohydride as a reducing agent. UV, FT-IR, XRD, NTA, and TEM methods were employed to obtain physicochemical characteristics of the products. The average diameter of Co nanoparticles was approximately 8.2 ± 3.4 nm. Their magnetic properties, including hysteresis loop, field-cooled, and zero field-cooled curves were investigated. The nanoparticles exhibit superparamagnetism at room temperature, accompanied by magnetic hysteresis below the blocking temperature.

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Immobilization of invertase on chitosan coated γ-Fe 2 O 3 magnetic nanoparticles to facilitate magnetic separation

Cited by 70 publications

References 39 publications

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles

Magnetic Cobalt and Cobalt Oxide Nanoparticles in Hyperbranched Polyester Polyol Matrix

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