Immobilization of invertase on sepharose-linked enzyme glycosyl recognizing polyclonal antibodies

A Study on the Comparative Stability of Insoluble Complexes of Glucose Oxidase Obtained with Concanavalin A and Specific Polyclonal Antibodies

“…Due to presence of inhibitory antibodies in the insoluble complexes of glucose oxidase, these complexes exhibited slightly lower enzyme activity. However, these observations are in agreement with several earlier findings (Jafri & Saleemuddin 1997;Saleemuddin 1999).…”

“…Our observations suggested that insoluble complexes of glucose oxidase prepared by using glycosyl-specific antiglucose oxidase polyclonal IgG were more stable to the inactivation induced by water miscible organic solvents as compared to other types of insoluble complexes of glucose oxidase (Figures 5 and 6). Several workers have already reported that glycosyl-specific antienzyme antibodies have pronounced stabilizing effect on enzymes (Shami et al 1991;Solomon et al 1987;Jafri et al 1995;Jafri & Saleemuddin 1997;Jan & Husain 2004). The stabilization of insoluble glucose oxidase complexes against various organic solvents could be possibly due to low water requirement or enhanced rigidity to the enzyme structure (Jan et al 2001;Jan & Husain 2004;Xin et al 2005).…”

Effective biochemical decomposition of chlorinated aromatic hydrocarbons with a biocatalyst immobilized on a natural enzyme support

“…Strong interactions, including covalent cross-linking, have previously been suggested to provide intramolecular forces that prevent conformational changes in enzymes (Sanjay and Sugunan, 2007). Enzyme protection due to immobilization has been reported by a number of other investigators (Bayramoglu and Arica, 2008;Jafri and Saleemuddin, 1997), who demonstrated that immobilization restricted denaturation or unfolding of enzymes in response to sudden pH changes. Lai and Lin (2005) suggested that the broadening of the optimal pH range may be due to the Donnan effect, whereby the ion (including H + ) distribution around the immobilized enzyme is altered.…”

Stem Bromelain: An Enzyme That Naturally Facilitates Oriented Immobilization

“…The preparation exhibited high enzyme activity and improved thermal stability. Other strategies employed to favourably orient enzymes on solid supports include binding to matrices precoupled with anti-enzyme monoclonal antibodies [2] or epitope specific polyclonal antibodies [3,4], use of enzymes genetically fused with specific binding proteins [5,6], or via avidin-biotin interactions [7]. The subject has also been reviewed [8,9].…”

“…These include oxidation of the sugars to generate aldehyde groups followed by coupling to supports bearing amino groups [11], affinity binding to supports precoupled to lectins [12] or anti-enzyme glycosyl antibodies [3] and binding to boronate affinity gels [13]. However, since most glycoenzymes contain multiple oligosaccharide chains, the preparations immobilized through the glycosyls may still be heterogenous.…”