Immobilization of isoamylase on carboxymethyl-cellulose and chitin

Liu, Hwai-Shen; Chen, Wei-Hsu; Lai, Jinn-Tsyy

doi:10.1007/bf02788807

Cited by 12 publications

(6 citation statements)

References 22 publications

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“…Because of these features it is used in many areas of biomedicine and biotechnology [ 30 , 31 ]. One of these fields is the immobilization of enzymes [ 32 , 33 , 34 ]. Krajewska [ 35 ] presents a wide-ranging review of the literature concerning the use of chitin as a support for many catalytic proteins.…”

Section: Introductionmentioning

confidence: 99%

Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization

et al. 2015

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Innovative materials were made via the combination of chitin and lignin, and the immobilization of lipase from Aspergillus niger. Analysis by techniques including FTIR, XPS and 13C CP MAS NMR confirmed the effective immobilization of the enzyme on the surface of the composite support. The electrokinetic properties of the resulting systems were also determined. Results obtained from elemental analysis and by the Bradford method enabled the determination of optimum parameters for the immobilization process. Based on the hydrolysis reaction of para-nitrophenyl palmitate, a determination was made of the catalytic activity, thermal and pH stability, and reusability. The systems with immobilized enzymes were found to have a hydrolytic activity of 5.72 mU, and increased thermal and pH stability compared with the native lipase. The products were also shown to retain approximately 80% of their initial catalytic activity, even after 20 reaction cycles. The immobilization process, using a cheap, non-toxic matrix of natural origin, leads to systems with potential applications in wastewater remediation processes and in biosensors.

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Section: Introductionmentioning

confidence: 99%

Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization

et al. 2015

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“…Targeting Wnt/β‐catenin pathway has been involved in cancer therapy of malignancies, including CRC . β‐catenin (CTNNB1) regulates cancer cell migration and invasion through transcriptionally activating ZEB1 (EMT‐related protein) and MMPs (invasive and metastatic factors .…”

Section: Discussionmentioning

confidence: 99%

Weichang'an suppressed migration and invasion of HCT116 cells by inhibiting Wnt/β‐catenin pathway while upregulating ARHGAP25

Zheng

et al. 2019

Biotech and App Biochem

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Evidence suggests that Weichang'an (WCA) inhibited the metastasis of colorectal cancer (CRC) in vitro and downregulates oncogenic β‐catenin; more intriguingly, we also found an upregulation of ARHGAP25 in this process. This study aimed to investigate the mechanisms by which WCA regulated CRC metastasis in vitro. Here, HCT116 cells were transfected with siRNAs to interfere ARHGAP25 expression. WCA decoction, XAV939 (a specific Wnt/β‐catenin pathway inhibitor), and LiCl (an activator for Wnt/β‐catenin pathway) were used for treatment. Cell migratory and invasive capacities were determined using Transwell chamber. The activation of Wnt/β‐catenin pathway was assessed by determining the expression of MMP7, MMP9, ZEB1, and β‐catenin. The study suggests that WCA inhibited the migration and invasion of HCT116 cells and suppressed the activation of Wnt/β‐catenin pathway, as evidenced by retarding MMP7, MMP9, ZEB1, and β‐catenin. However, siRNA–ARHGAP25 resulted in the opposite. In siRNA–ARHGAP25‐transfected HCT116 cells, WCA (0.4 mg/mL) induced the antimetastatic effects and the inactivation of Wnt/β‐catenin pathway was remarkably reversed with additional LiCl treatment. Our study concludes that inhibiting Wnt/β‐catenin pathway while promoting ARHGAP25 was the mechanism, whereby WCA retarded migration and invasion of CRC in vitro.

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“…N-Isopropylacrylamide (NIP), N-hydroxysuccinimidyl acrylate (NAS) were synthesized as described. 11,12 Ammonium persulfate and N,N,NA,NA-tetramethylethylenediamine (TEMED) were purchased from TCI-EP (Tokyo, Kasei) and used without further purification. Phosphate buffered saline (PBS) solutions at pH 7.4 and 8.0 at a concentration of 0.1 mol cm 23 were used.…”

Section: Methodsmentioning

confidence: 99%

Mimetic-enzyme fluorescence immunoassay using a thermal phase separating polymer

Zhu¹,

Xu²,

Li³

et al. 1998

Analyst

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Poly-N-isopropylacrylamide (PNIP), a water-soluble, thermally precipitated synthetic polymer, has been conjugated together with a monoclonal antibody and utilized as a novel separation method for an immunoassay. PNIP precipitates out of water above a critical temperature of 31 degrees C, enabling a polymer-bound immune complex to be separated from the solution. These characteristics were used to develop a novel polymer-mimetic enzyme immunoassay method for determination of alpha-1-fetoprotein (AFP) with hemin as a labeling reagent to catalyze the reaction of p-hydroxyphenyl acetic acid (HPA) and hydrogen peroxide in alkaline medium. After a one-step competitive immunoreaction, the polymer-antibody-antigen-hemin conjugate moiety was determined by coupling the fluorogenic reaction of HPA and hydrogen peroxide, The calibration graph for AFP was linear over the range of 0-380 ng cm(-3) with a detection limit of 1.0 ng cm(-3). This method combines some advantages of both homogeneous and heterogeneous immunoassays, and has been applied to determine AFP in human blood serum with satisfactory results

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Immobilization of isoamylase on carboxymethyl-cellulose and chitin

Cited by 12 publications

References 22 publications

Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization

Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization

Weichang'an suppressed migration and invasion of HCT116 cells by inhibiting Wnt/β‐catenin pathway while upregulating ARHGAP25

Mimetic-enzyme fluorescence immunoassay using a thermal phase separating polymer

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