Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neuron culture

Young, Tai‐Horng; Lu, Jui-Nan; Lin, Dar‐Jong; Chang, Chi-Lin; Chang, Hung-Sheng; Cheng, Liao‐Ping

doi:10.1016/j.desal.2007.09.079

Cited by 22 publications

(34 citation statements)

References 13 publications

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“…Their results showed the potential of galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. Young et al (2008) fabricated microporous PVDF membranes for nerve tissue engineering by immobilizing L-lysine covalently on the surface of PVDF membrane. PC12 cells cultured on L-lysine/PVDF membranes showed good cell adhesion and proliferation on L-lysine/PVDF membranes, suggesting its usefulness in the development of strategies to promote the regrowth and regeneration of nervous tissue.…”

Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

618

389

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Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed.

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Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

618

389

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“…The carboxyl groups of the electrografted diazonium salt were activated by incubating the functionalized electrodes in a 26 mM EDC and 35 mM sulfo-NHS solution in 100 mM MES buffer (pH 4.5) for 1 h. In order to conjugate the carboxy-fucntionalized electrode with the carboxy-modified ligands, a lysine spacer was intercalated in between, by using its two amino functionalities to form amido bonds [22][23][24][25]. The surface activated groups reacted overnight with the α-amine group of the lysine at 4°C.…”

Section: Covalent Immobilization Of Crown Ethers Via Carbodiimide Coumentioning

confidence: 99%

Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu(II)

Serrano

González‐Calabuig

Valle

2015

Talanta

106

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This work describes the immobilization of 4-carboxybenzo-18-crown-6 (CB-18-crown-6) and 4-carboxybenzo-15-crown-5 (CB-15-crown-5) assisted by lysine on aryl diazonium salt monolayers anchored to the surface of graphite-epoxy composite electrodes (GEC), and their use for the simultaneous determination of Cd(II), Pb(II) and Cu(II) by differential pulse anodic stripping voltammetry (DPASV). These modified electrodes display a good repetitivity and reproducibility with detection and quantification limits at levels of µg L -1 (ppb), confirming their suitability for the determination of Cd(II), Pb(II) and Cu(II) ions in environmental samples. The overlapped nature of the multimetal stripping measurements was resolved by employing the two-sensor array CB-15-crown-5-GEC and CB-18-crown-6-GEC, since the metal complex selectivity exhibited by the considered ligands could add * e-mail: manel.delvalle@uab.es; Phone: (+34) 93 581 10 17; Fax: (+34) 93 581 24 77 *Manuscript This is the author's version of a work that was accepted for publication in Talanta (Ed. Elsevier). Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in: Serrano, N., González, A. and del Valle, M. "Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu (II)" in Talanta, vol. 138 (June 2015), p. 130-137. DOI 10.1016/j.talanta.2015 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

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“…In the present work, attempts have been made to immobilize L-lysine on a PVDF membrane surface with either a dense or porous morphology [10]. The synthesis routes are shown in Schemes 1 and 2.…”

Section: Introductionmentioning

confidence: 99%

“…Scheme 1 indicates the grafting reaction of glycidyl methacrylate (GMA) on PVDF substrate by means of the plasma-induced polymerization method. Briefly, PVDF membrane was plasma-irradiated and then exposed to air to form peroxides [9][10][11][12][13][14][15][16][17], which subsequently decomposed to give free radicals, -CO • , at an elevated temperature or in the presence of Fe 2+ (e.g., Mohr salt). These radicals served to initiate the free radical polymerization of GMA into PGMA on the membrane surface.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of L-Lysine on Microporous PVDF Membranes for Neuron Culture

Young

Lin

et al. 2009

Journal of Biomaterials Science, Polymer Edition

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Microporous poly(vinylidene fluoride) (PVDF) membranes with dense or porous surface were prepared by immersion precipitation of PVDF/TEP solutions in coagulation baths containing different amounts of water. Onto the membrane surface, poly(glycidyl methacrylate) (PGMA) was grafted by plasma-induced free radical polymerization. Then, L-lysine was covalently bonded to the as-grafted PGMA through ring-opening reactions between epoxide and amine to form amino alcohol. The highest attainable graft density of PGMA on a PVDF membrane was 0.293 mg/cm2. This was obtained when the reaction was carried out on a porous surface under an optimized reaction condition. For immobilization of L-lysine, the yield was found to depend on the reaction temperature and L-lysine concentration. The maximal yield was 0.226 mg/cm2, a value considerably higher than reported in the literature using other immobilization methods. Furthermore, neurons were cultured on L-lysine-immobilized PVDF membranes. The results indicated that these membrane surfaces were suited to the growth of neurons, with a MTT value higher than that of the standard culture dish.

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Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neuron culture

Cited by 22 publications

References 13 publications

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu(II)

Immobilization of L-Lysine on Microporous PVDF Membranes for Neuron Culture

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