Immobilization of l-lysine α-oxidase on gold-mercaptopropionic acid self-assembled monolayer: Preparation and electrochemical characterization

Shervedani, Reza Karimi; Hemmatian, Zahra; Hatefi-Mehrjardi, Abdolhamid

doi:10.1016/j.bioelechem.2009.02.011

Cited by 18 publications

(8 citation statements)

References 50 publications

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“…Some literature report a similar increase in the activity of the enzyme LyOx in weakly basic media and also emphasize higher electrode responses at higher pH values , . Different pH values such as 5.0 , 6.0 , 7.0 , , , 7.4 , , 7.5 , , 8.0 and 9.0 were also reported for lysine biosensors. However, it is important to highlight that the effect of pH on the biosensor response was not investigated in detail at pH values over 9.0.…”

Section: Resultsmentioning

confidence: 62%

“…L‐lysine is the most easily damaged of the essential amino acids and food processing and storage can result in a decrease in the lysine content of the food . Therefore, L‐lysine content is an important marker for the nutritional value of food . Various analytical techniques have been reported for L‐lysine determination, e. g. liquid chromatography , reverse phase liquid chromatography , high performance thin layer chromatography and densitometry .…”

Section: Introductionmentioning

confidence: 99%

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Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L‐lysine Determination

2017

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A novel and sensitive amperometric biosensor for L‐lysine determination based on a glassy carbon electrode (GCE) modified with graphene (GR) and redox polymer poly(vinylferrocene) (PVF) was constructed. L‐lysine‐α‐oxidase was immobilized onto the modified GCE by a glutaraldehyde/bovine serum albumin cross‐linking procedure. SEM, CV and EIS were used for the characterization of the surface morphology and stepwise fabrication processes of PVF/GR composite. Optimal composition of the biosensor and experimental conditions that affect the performance of the biosensor are discussed. The effect of buffer pH on biosensor response was studied in detail over a wide pH range. L‐lysine biosensor displayed a linear range of 9.9×10−7 ‐ 3.1×10−4 M with a low detection limit of 2.3×10−7 M and KMapp value of 0.4 mM. The L‐lysine biosensor was tested using pharmaceutical sample and cheese with satisfactory results.

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Section: Resultsmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L‐lysine Determination

2017

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“…The most frequent application of SAMs in electroanalytical chemistry is in the development of sensors and biosensors where the SAM is used to impart selectivity onto an electrode for a particular analyte. [21][22][23][24][25][26][27][28][29][30] To our knowledge, no study has been published so far reporting on the simultaneous determination of DA and UA using 2-(3,4-dihydroxyphenyl)-1,3-dithialone self-assembled monolayer (DHPD-SAM). Here in continuation of our studies concerning the preparation of modified electrodes, [31][32][33][34][35][36][37][38] we report the preparation and application of DHPD-SAM as a new electrode for the electrocatalytic determination of DA in phosphate buffer solution.…”

Section: Introductionmentioning

confidence: 99%

Application of 2-(3,4-dihydroxyphenyl)-1,3-dithialone self-assembled monolayer on gold electrode as a nanosensor for electrocatalytic determination of dopamine and uric acid

Mazloum-Ardakani

Beitollahi

Amini

et al. 2011

Analyst

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The electrooxidation of dopamine (DA), uric acid (UA) and their mixture on a gold electrode modified by a self-assembled monolayer of 2-(3,4-dihydroxyphenyl)-1,3-dithialone has been studied by cyclic voltammetry (CV), chronoamperometry and differential pulse voltammetry (DPV). CV was used to investigate the redox properties of the modified electrode at various scan rates and the apparent charge transfer rate constant (k(s)), and transfer coefficient (α) were calculated. The mediated oxidation of DA at the modified electrode under the optimum condition (pH = 7.0) in CV occurs at a potential about 220 mV less positive than that of the unmodified gold electrode. The values of electron transfer coefficients (α), catalytic rate constant (k) and diffusion coefficient (D) were calculated for DA, using electrochemical methods. DPV exhibited a linear dynamic range over the concentration range of 0.2-250.0 μM and a detection limit (3σ) of 0.07 μM for DA. The modified electrode was used for simultaneous determination of DA and UA by DPV. The results showed that the electrode is highly efficient for the catalytic electrooxidation of DA and UA, leading to a remarkable peak resolution (~350 mV) for two compounds. The electrode was used for the determination of DA in an injection sample.

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“…2,10 Biosensing methods are comparatively more simple, rapid, sensitive, specific, inexpensive, portable and reusable. Lysine biosensors employing a nylon/collagen membrane mounted on different electrodes, [11][12][13][14][15] aminosilylated glass, 5 polycarbonate membranes, 16 activated alkylaminated controlled pore glass, 17 poly(o-phenylenediamine) membranes, 18,3 polyurethane hydrogel, 19 PTFE (polytetrafluoroethylene) tube, 6 imidodiacetic acid chelating beads on glassy carbon foil 20 and gold-mercaptopropionic acid self assembled monolayers 21 have met with limited success, due to the leakage of enzyme from the support during washing.…”

Section: Introductionmentioning

confidence: 99%

Development of amperometric lysine biosensors based on Au nanoparticles/multiwalled carbon nanotubes/polymers modified Au electrodes

Chauhan

Singh

Narang

et al. 2012

Analyst

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The construction of two amperometric l-lysine biosensors is described in this study. The construction comprises the covalent immobilization of lysine oxidase (LOx) onto nanocomposite composed of gold nanoparticles (AuNPs) and carboxylated multiwalled carbon nanotubes (c-MWCNT), decorated on (i) polyaniline (PANI) and (ii) poly 1,2 diaminobenzene (DAB), electrodeposited on Au electrodes. The biosensors were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) and electrochemical impedance spectroscopy (EIS) studies. The optimum response (current) was observed within 2 s at pH 7.0 and 25 °C for LOx/AuNPs/c-MWCNT/PANI/Au, and 4 s at pH 7.0 and 30 °C for LOx/AuNPs/c-MWCNT/DAB/Au electrodes. There was a linear relationship between current and lysine concentration ranging from 5.0 to 600 μM for LOx/AuNPs/c-MWCNT/PANI/Au with a detection limit of 5.0 μM, and 20 to 600 μM for the LOx/AuNPs/c-MWCNT/DAB/Au electrode with a detection limit of 20 μM. The PANI modified electrode was in good agreement with the standard HPLC method, with a better correlation (r = 0.992) compared to the DAB modified electrode (r = 0.986). These observations revealed that the PANI modified Au electrode was better than the DAB modified electrode, and hence it was employed for the determination of lysine in milk, pharmaceutical tablets and sera. The PANI modified electrode showed a half life of 120 days, compared to that of 90 days for the DAB modified electrode, after their 100 uses, when stored at 4 °C.

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Immobilization of l-lysine α-oxidase on gold-mercaptopropionic acid self-assembled monolayer: Preparation and electrochemical characterization

Cited by 18 publications

References 50 publications

Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L‐lysine Determination

Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L‐lysine Determination

Application of 2-(3,4-dihydroxyphenyl)-1,3-dithialone self-assembled monolayer on gold electrode as a nanosensor for electrocatalytic determination of dopamine and uric acid

Development of amperometric lysine biosensors based on Au nanoparticles/multiwalled carbon nanotubes/polymers modified Au electrodes

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