Immobilization of laccase from Aspergillus oryzae on graphene nanosheets

Skoronski, Éverton; Souza, Diego Hoefling; Ely, Cyntia; Broilo, Felipe; Fernandes, Mylena; Fúrigo, Agenor; Ghislandi, Marcos Gomes

doi:10.1016/j.ijbiomac.2017.02.076

Cited by 75 publications

(18 citation statements)

References 36 publications

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“…To research the effect of pH on the laccase immobilization, 10 mg support was put into 8 g/L laccase solutions with pH ranged from 3.0 to 7.0 at 25 ± 1 °C and contact time was 2 h. Generally, the strength of the acting force between the support and laccase during an adsorption process was decided by the charge properties of the support surface and the laccase molecule [26]. However, laccase enzyme shows different charge characteristics under different pH conditions, and the change of the electrostatic association will influence the recovered activity and laccase immobilization yield [27].…”

Section: Resultsmentioning

confidence: 99%

Immobilizing Laccase on Modified Cellulose/CF Beads to Degrade Chlorinated Biphenyl in Wastewater

Xia

et al. 2018

Polymers

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Novel modified cellulose/cellulose fibril (CF) beads (MCCBs) loaded with laccase were prepared to degrade polychlorinated biphenyls (PCBs) in wastewater. The proper porous structure in MCCBs was achieved by introducing nano CaCO 3 (as a pore forming agent) in cellulose/CF (CCBs) beads during the preparation process. Cellulose/CF composite beads were modified by maleic anhydride to introduce carboxyl groups. Laccase was immobilized on the MCCBs through electrostatic adsorption and covalent bonding. The effects of pH, laccase concentration and contact time on immobilization yields and recovered activity were investigated. The best conditions were pH 4, concentration 16 g/L and contact time 3 h. The immobilized laccase under these conditions showed a good performance in thermal and operational stability. The laccase immobilized on MCCB beads can remove 85% of 20 mg/L 4-hydroxy-3,5-dichlorobiphenyl (HO-DiCB) in wastewater. The results demonstrated that MCCBs, as a new type of green-based support, are very promising in material immobilizing laccase. This technology may be of potential advantage for the removal of polychlorinated biphenyls in wastewater from an environmental point of view.

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Section: Resultsmentioning

confidence: 99%

Immobilizing Laccase on Modified Cellulose/CF Beads to Degrade Chlorinated Biphenyl in Wastewater

Xia

et al. 2018

Polymers

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“…The structure of graphene-based nanomaterials can be an effective transducer since it allows the direct electron transfer between enzymes and electrodes [ 19 ]. Furthermore, graphene-based materials have been shown to be excellent substrates for increasing thermal stability, enzymatic activity, and for enzyme immobilization [ 105 – 107 ].…”

Section: Graphene-based Nanomaterials and Enzymesmentioning

confidence: 99%

Recent advances in graphene-based biosensor technology with applications in life sciences

et al. 2018

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Graphene’s unique physical structure, as well as its chemical and electrical properties, make it ideal for use in sensor technologies. In the past years, novel sensing platforms have been proposed with pristine and modified graphene with nanoparticles and polymers. Several of these platforms were used to immobilize biomolecules, such as antibodies, DNA, and enzymes to create highly sensitive and selective biosensors. Strategies to attach these biomolecules onto the surface of graphene have been employed based on its chemical composition. These methods include covalent bonding, such as the coupling of the biomolecules via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide reactions, and physisorption. In the literature, several detection methods are employed; however, the most common is electrochemical. The main reason for researchers to use this detection approach is because this method is simple, rapid and presents good sensitivity. These biosensors can be particularly useful in life sciences and medicine since in clinical practice, biosensors with high sensitivity and specificity can significantly enhance patient care, early diagnosis of diseases and pathogen detection. In this review, we will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensors able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases.

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“…The exceptional reusability of these biocatalytic systems is related to the stability and durability of the chitinous scaffolds [26] as well as the ability of this material to protect the immobilized enzyme against inactivation as a result of process conditions [85]. Furthermore, the enzyme-support interactions protect the enzyme against elution from the matrix, as tetracycline is washed out from the scaffolds between repeated uses [86]. Although the data show that the immobilized laccase is stable during repeated use, the decrease in tetracycline removal is related mainly to inactivation of the enzyme due to its inhibition by the macromolecular products of the reaction [87], although partial elution of the laccase from the support should not be excluded.…”

Section: Removal Of Tetracyclinementioning

confidence: 99%

3D Chitin Scaffolds from the Marine Demosponge Aplysina archeri as a Support for Laccase Immobilization and Its Use in the Removal of Pharmaceuticals

et al. 2020

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For the first time, 3D chitin scaffolds from the marine demosponge Aplysina archeri were used for adsorption and immobilization of laccase from Trametes versicolor. The resulting chitin–enzyme biocatalytic systems were applied in the removal of tetracycline. Effective enzyme immobilization was confirmed by scanning electron microscopy. Immobilization yield and kinetic parameters were investigated in detail, in addition to the activity of the enzyme after immobilization. The designed systems were further used for the removal of tetracycline under various process conditions. Optimum process conditions, enabling total removal of tetracycline from solutions at concentrations up to 1 mg/L, were found to be pH 5, temperature between 25 and 35 °C, and 1 h process duration. Due to the protective effect of the chitinous scaffolds and stabilization of the enzyme by multipoint attachment, the storage stability and thermal stability of the immobilized biomolecules were significantly improved as compared to the free enzyme. The produced biocatalytic systems also exhibited good reusability, as after 10 repeated uses they removed over 90% of tetracycline from solution. Finally, the immobilized laccase was used in a packed bed reactor for continuous removal of tetracycline, and enabled the removal of over 80% of the antibiotic after 24 h of continuous use.

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Immobilization of laccase from Aspergillus oryzae on graphene nanosheets

Cited by 75 publications

References 36 publications

Immobilizing Laccase on Modified Cellulose/CF Beads to Degrade Chlorinated Biphenyl in Wastewater

Immobilizing Laccase on Modified Cellulose/CF Beads to Degrade Chlorinated Biphenyl in Wastewater

Recent advances in graphene-based biosensor technology with applications in life sciences

3D Chitin Scaffolds from the Marine Demosponge Aplysina archeri as a Support for Laccase Immobilization and Its Use in the Removal of Pharmaceuticals

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