Immobilization of laccase on epoxy-functionalized silica and its application in biodegradation of phenolic compounds

Mohammadi, Mehdi; As’habi, Mohammad Ali; Salehi, Peyman; Yousefi, Maryam; Nazari, Mahboobeh; Brask, Jesper

doi:10.1016/j.ijbiomac.2017.12.102

Cited by 131 publications

(47 citation statements)

References 38 publications

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“…Silicates are structurally stable, relatively cheap, and chemically resistant to organic solvents. Mohammadi et al [66] examined laccase immobilization on silica particles functionalized with epoxy groups. Covalently immobilized laccase tripled its stability at extremely high temperatures, retaining 96% of its activity at 35°C, where the free enzyme showed only 35% active at the same temperature.…”

Section: Removal Of Phenolsmentioning

confidence: 99%

Enhanced Wastewater Treatment by Immobilized Enzymes

et al. 2021

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Purpose of Review In the presented review, we have summarized recent achievements on the use of immobilized oxidoreductases for biodegradation of hazardous organic pollutants including mainly dyes, pharmaceuticals, phenols, and bisphenols. In order to facilitate process optimization and achievement of high removal rates, effect of various process conditions on biodegradation has been highlighted and discussed. Recent Findings Current reports clearly show that immobilized oxidoreductases are capable of efficient conversion of organic pollutants, usually reaching over 90% of removal rate. Further, immobilized enzymes showed great recyclability potential, allowing their reuse in numerous of catalytic cycles. Summary Collected data clearly indicates immobilized oxidoreductases as an efficient biocatalytic tools for removal of hazardous phenolic compounds, making them a promising option for future water purification. Data shows, however, that both immobilization and biodegradation conditions affect conversion efficiency; therefore, process optimization is required to achieve high removal rates. Nevertheless, we have demonstrated future trends and highlighted several issues that have to be solved in the near-future research, to facilitate large-scale application of the immobilized oxidoreductases in wastewater treatment.

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Section: Removal Of Phenolsmentioning

confidence: 99%

Enhanced Wastewater Treatment by Immobilized Enzymes

et al. 2021

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“…The results demonstrated the effective removal of different classes of phenolic compounds (phenol, chloro-, trichloro-and dichlorophenol, cresols, dimethyl-, 2-methyl-4,6-dinitro-, 4-nitro-, tetrachloroand pentachlorophenol). Silica-based material was also been studied by Mohammadi et al [85]. In this case, the silica was modified with epoxy groups, and the laccase was covalently immobilized using nucleophilic attack by the amino groups of the laccase on the epoxy groups of the support.…”

Section: Phenol Compounds and Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment

Kołodziejczak-Radzimska

Nghiem

Jesionowski

2021

Curr Pollution Rep

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Purpose of Review Untreated wastewater discharge can significantly and negatively impact the state of the environment. Rapid industrialization and economic development have directly contributed to land and water pollution resulting from the application of many chemicals such as organic dyes, pharmaceuticals, and industrial reagents. The removal of these chemicals before effluent discharge is crucial for environmental protection. This review aims to explore the importance of functionalized materials in the preparation of biocatalytic systems and consider their application in eliminating water pollutants. Recent Findings Wastewater treatment methods can be classified into three groups: (i) chemical (e.g., chemical oxidation and ozonation), (ii) physical (e.g., membrane separation and ion exchange), and (iii) biological processes. Biological treatment is the most widely used method due to its cost-effectiveness and eco-friendliness. In particular, the use of immobilized enzymes has recently become more attractive as a result of scientific progress in advanced material synthesis. The selection of an appropriate support plays an important role in the preparation of such biologically active systems. Recent studies have demonstrated the use of various materials for enzyme immobilization in the purification of water. Summary This review identifies and discusses different biocatalytic systems used in the enzymatic degradation of various water pollutants. Materials functionalized by specific groups can serve as good support matrices for enzyme immobilization, providing chemical and thermal stability to support catalytic reactions. Enzymatic biocatalysis converts the pollutants into simpler products, which are usually less toxic than their parents. Due to immobilization, the enzyme can be used over multiple cycles to reduce the cost of wastewater treatment. Future studies in this field should focus on developing new platforms for enzyme immobilization in order to improve degradation efficiency.

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“…As a clean, effective and green biocatalyst, its theoretical research and technical application have been developed for decades. Due to their low specificity, several organic compounds such as polycyclic aromatic hydrocarbons [ 1 ], aromatic amines [ 2 ], phenolic compounds [ 3 ], etc. are the substrates of laccase [ 4 ].…”

Section: Intorductionmentioning

confidence: 99%