Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole–polyvinylsulphonate composite films for application to lactate biosensors

Chaubey, Asha; Gerard, Manju; Singhal, Rahul; Singh, Virendra Bahadur; Malhotra, Bansi D.

doi:10.1016/s0013-4686(00)00658-7

Cited by 110 publications

(65 citation statements)

References 29 publications

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“…Furthermore, if this comparison is made with respect to LDH biosensors constructed from electrodes modified with other conducting polymers [12,13], it can be deduced that biosensors prepared with polypyrrole-poly(vynil) sulfonate and polyaniline allowed the use of detection potentials around þ 200 mV, but the detection limits obtained were much higher (100 and 50 mM, respectively) than that achieved with the biosensor based on the use of the hybrid material reported in this work.…”

Section: Analytical Characteristics and Biosensor Performancementioning

confidence: 71%

Lactate Dehydrogenase Biosensor Based on an Hybrid Carbon Nanotube‐Conducting Polymer Modified Electrode

et al. 2009

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A lactate biosensor constructed by immobilization of the enzyme lactate dehydrogenase (LDH) on a glassy carbon electrode modified with a hybrid material composed of the conducting polymer poly (3-methylthiophene) (P3 MT), electrodeposited on the electrode surface, and multiwalled carbon nanotubes (MWCNTs), is reported. The excellent characteristics of the electrode modified with the hybrid material toward the electrochemical oxidation of NADH allowed the construction of a robust LDH electrochemical biosensor able to operate at a working potential as low as þ 300 mV without the need for redox mediator. Experimental variables affecting the performance of the biosensor: amount of LDH immobilized on the modified electrode surface, buffer composition and working pH value, MWCNTs loading in the hybrid material and NAD þ concentration were optimized. Under the optimized conditions, a linear calibration graph for lactate was obtained over the 1.0 Â10 À6 À5.0 Â10 À4 M (r ¼0.9993) concentration range, with a detection limit of 5.6 Â 10 À7 M. The biosensor design showed good repeatability of the measurements, good reproducibility in the inter-biosensor assays and a good selectivity against other organic acids, especially when the biosensor is coated with a Nafion film.

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Section: Analytical Characteristics and Biosensor Performancementioning

confidence: 71%

Lactate Dehydrogenase Biosensor Based on an Hybrid Carbon Nanotube‐Conducting Polymer Modified Electrode

et al. 2009

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“…43 The Pt plate electrode was immersed in 10 mL of solution of 0.1 M pyrrole and 2.5 mL (25%) of sodium polyvinylsulfonate. The solution was purged with argon in order to remove the oxygen.…”

Section: Preparation Of Pt/ppy-pvs Film Electrodementioning

confidence: 99%

Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode

Çolak¹,

Arslan²

2015

Turk J Chem

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“…It is worth comparing the analytical performance of the present l-lactate biosensor with other dehydrogenase llactate biosensors based on polymers [20,23,27,29], optical fiber [15] trienzyme [20], etc. The sensitivity of these biosensors is very low and they operate at relatively high potential, which invites the interference due to other easily oxidizable species like AA and UA.…”

Section: Amperometric L-lactate Biosensormentioning

confidence: 99%

“…On the other hand, the sensing l-lactate using LDH involves the detection of enzymatically generated NADH (Eq. 2) [20,23,27,29].…”

Section: Introductionmentioning

confidence: 99%

Amperometric L‐Lactate Biosensor Based on Gold Nanoparticles

Jena

Raj

2007

Electroanalysis

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A novel amperometric biosensor for the sensing of l-lactate is developed using l-lactate dehydrogenase (LDH) and hydroxylamine enlarged gold nanoparticles (GNPs). LDH and GNPs have been integrated with the sol -gel 3-D silicate network derived from 3-(mercaptopropyl)trimethoxysilane (MPTS). The biosensing of l-lactate is based on the electrocatalytic determination of enzymatically generated NADH by GNPs of the integrated assembly. The GNPs on the network efficiently catalyze the oxidation of NADH at À 0.065 V, which is ca. 915 mV less positive than on the bulk Au electrode. The biosensor was characterized in terms of the effects of enzyme loading, solution pH, and cofactor concentration. The integrated biosensor was successfully utilized for the amperometric sensing of l-lactate and it shows excellent sensitivity with a detection limit of 100 nM. The common interfering electroactive compounds in the biological system do not interfere the amperometric measurement of l-lactate. This biosensor linearly responds to l-lactate in the range of 0 -0.8 mM and the sensitivity of the electrode was 0.446 nA/nM. Excellent reproducibility, long time storage and operational stability have been achieved.

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Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole–polyvinylsulphonate composite films for application to lactate biosensors

Cited by 110 publications

References 29 publications

Lactate Dehydrogenase Biosensor Based on an Hybrid Carbon Nanotube‐Conducting Polymer Modified Electrode

Lactate Dehydrogenase Biosensor Based on an Hybrid Carbon Nanotube‐Conducting Polymer Modified Electrode

Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode

Amperometric L‐Lactate Biosensor Based on Gold Nanoparticles

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