Immobilization of Levansucrase: Strategies and Biotechnological Applications

Abstract

Immobilization is an excellent tool for enzymatic stabilization, improving the biocatalytic processes, allowing the reuse of the enzyme and promoting an easier separation of the molecule of interest. Currently, new enzymatic bonding processes are arising on solid supports, based on classical immobilization methods. Amongst the supports used, chitosan is a polysaccharide that offers a unique set of characteristics, as biocompatibility, biodegradability, non-toxicity and antibacterial properties. Thus, many enzymes has being immobilized on this support, including levansucrase, that is able to synthesize levan and fructooligosaccharides, two important biomolecules which have beneficial health properties. These review present different methods of immobilization (physical adsorption, entrapment, crosslinking and covalent bonding) for fructosyltransferases, as well as different immobilization matrices that can be applied in biotechnological processes. However, studies are still needed in order to adopt efficient immobilization techniques, in which the biocatalyst remains more stable, in order to become the process attractive to the industrial sector.

Thermally Stable and Reusable Silica and Nano-Fructosome Encapsulated CalB Enzyme Particles for Rapid Enzymatic Hydrolysis and Acylation

“…Therefore, levan-encapsulated enzymes or proteins have been studied and utilized to solve problems of reduced activities of proteinaceous substances such as enzymes. In addition, they are resistant to organic solvents [ 31 , 32 , 33 , 34 ]. Furthermore, we developed carboxymethyl levan (CML)-based nanocomposites dubbed ‘nanofructosome (NF)’ and demonstrated the effects of NF particles by entrapping lipase [ 35 ].…”