Immobilization of lipase on methyl-modified silica aerogels by physical adsorption

Gao, Siliang; Wang, Yujun; Wang, Tao; Luo, Guangsheng; Dai, Yiyang

doi:10.1016/j.biortech.2008.06.060

Cited by 65 publications

(26 citation statements)

References 13 publications

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“…Macroporus acrylic polymer resins such as Amberlite XAD-7 (Takaç and Bakkal 2007) can be used for enzyme adsorption, while CaL-B immobilised on VP OC1600 (Bayer) is widely used for the production of speciality chemicals (Miletić et al 2009). Alternatively, silica-based materials such as modified aerogels (Gao et al 2009) or Celite can be used. Chaplin et al (2002) immobilised Pseudomonas fluorescens lipase on Celite for the resolution of menthol from an eight diastereomer mix in an organic solvent based reaction.…”

Section: Support Based Immobilisationmentioning

confidence: 99%

Advances in enzyme immobilisation

Brady

Jordaan²

2009

Biotechnol Lett

750

482

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Improvements in current strategies for carrier-based immobilisation have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self-immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.

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Section: Support Based Immobilisationmentioning

confidence: 99%

Advances in enzyme immobilisation

Brady

Jordaan²

2009

Biotechnol Lett

750

482

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show abstract

“…[7][8][9][10] Although it depends on the feasibility, covalent coupling is favored as the desorption of enzymes can be avoided compared to other techniques, and as a result, the reusability feature is improved. The large surface area and ease of preparation in different geometric configurations and the flexibility to introduce new functional groups makes these membrane systems more attractive.…”

Section: Introductionmentioning

confidence: 98%

Comparative study of the hydrolysis of different oils by lipase‐immobilized membranes

Gupta

Ingole

Singh

et al. 2011

J of Applied Polymer Sci

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“…The support should also be thermally stable, chemically durable, resistant to contamination, and available at a reasonable cost (Gemeiner, 1992). Physical methods, especially adsorption, have an advantage over chemical methods for the immobilization of enzymes onto carriers in that physical adsorption is simple, less expensive and can retain high catalytic activity (Huang et al, 2008;Gao et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Comparative studies of the stability of free and immobilized inulinase from Kluyveromyces marxianus NRRL Y-7571 in aqueous-organic solutions

Risso

Mazutti²,

Costa

et al. 2010

Braz. J. Chem. Eng.

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-Enzymes have been extensively used in organic solvents to catalyze a variety of reactions of biological and industrial significance. In this work, the characteristics of free and immobilized inulinase were investigated in buffered solutions of butyl acetate. The influences of the organic solvent content on the optimal temperature and pH, the stabilities to temperature and pH and the kinetic parameters were systematically evaluated. The results showed that the organic solvent content had no effect on the optimal pH, either in the free or immobilized inulinase. For the immobilized enzyme, the optimal temperatures ranged from 55°C to 60°C, depending on the content of butyl acetate. At higher butyl acetate content, the stability of the immobilized enzyme increased for both pH and temperature. The organic solvent showed the tendency to increase the values of the kinetic parameters K m and v max for both free and immobilized inulinase.

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Immobilization of lipase on methyl-modified silica aerogels by physical adsorption

Cited by 65 publications

References 13 publications

Advances in enzyme immobilisation

Advances in enzyme immobilisation

Comparative study of the hydrolysis of different oils by lipase‐immobilized membranes

Comparative studies of the stability of free and immobilized inulinase from Kluyveromyces marxianus NRRL Y-7571 in aqueous-organic solutions

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