Immobilization of lipase onto micron-size magnetic beads

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology

“…It can also reduce the capital and operation costs [100]. Due to the functionalization [101] of enzyme and its suitable microenvironment, magnetic materials were often embedded in organic polymer or inorganic silica to form hybrid support [102]. Recently, because of the low enzyme loading on the conventional magnetic beads, further attention was paid to the magnetic mesoporous support [103].…”

Lipase NS81006 immobilized on Fe3O4 magnetic nanoparticles for biodiesel production

“…Glutaraldehyde concentration is less than 10% (v/v) answered in deficient activation of the functionalized magnetic nanoparticles, while higher concentrations affected excessive coupling reagent of glutaraldehyde, which might be a steric effect for lipase. Due to steric hindrance effect, the activity of the immobilized lipase ebbing [27]. Xie et al achieved the maximum immobilization efficiency and activity recovery like above 80% and 65% at 10% glutaraldehyde concentration by lipase (Lipozyme-TL) immobilized on APTES-Fe3O4 [5].…”

Enzymatic Interesterification of Soybean Oil and Methyl Stearate Blends Using Lipase Immobilized on Magnetic Fe3O4/SBA-15 Composites as a Biocatalyst

“…After immobilization of the lipase on the magnetic particles Fig. 1, spectrum d , the IR bands responsible for the lipase that was chemically covalent-bonded to the magnetic particles, were observed at 1636 cm 1 for amide and at 1549 cm 1 for amide 27 . In addition, the IR peak appeared at 1662 cm 1 could be assignable to stretching vibrations of C N bond, indicating the formation of schiff base as a result of the reaction between carbonyl group of glutaraldehyde and amine group of lipase 28 .…”