Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate

Abstract

The immobilization of enzyme is one of the key issues both in the field of enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) in alginate carrier. In the presence of calcium cation, the enzyme-alginate suspension could be cross-linked to form beads with porous structure at room temperature, and the enzyme CALB was dispersed in the beads. Activity of the enzyme-alginate composite was verified by enzymatic hydrolysis reaction of p-nitrophenol butyrate in aqueous phase. The effects of reaction parameters such as temperature, pH, embedding and lyophilized time on the reactive behavior were discussed. Reuse cycle experiments for the hydrolysis of pnitrophenol butyrate demonstrated that activity of the enzyme-alginate composite was maintained without marked deactivation up to 6 repeated cycles.

Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies

“…Among others, the cleavage of the ester bond is of great importance for biodiesel production, synthesis of pharmaceuticals and cosmetics and in many branches of the food industry [2]. Low thermal stability, narrow pH range and a fast decrease in the catalytic activity are the main factors that limit the industrial use of lipases [3,4]. Lipase immobilization has been proposed to improve its stability and activity [5,6].…”

Rhizomucor miehei Lipase-Immobilized Sodium Alginate Membrane Preparation and Usage in a Pervaporation Biocatalytic Membrane Reactor

“…Before specifying the total amount of lipase, optimization tests were applied. Alginate was a bio-based polymer and it was completely compatible with lipases 40,47 . As can be seen in Figure 4, the highest conversion values were obtained with RM1 at all temperatures when M = 1.…”

“…It is also produced from bio-based feedstock 39 . Sodium alginate was selected as the polymer due to its well-known adoption property with lipase 40,[17][18][19] . Lipases were immobilized on alginate surface by entrapment and cross-linking technique reported in the previous study 36 .…”

Microencapsulation of maltogenicα-amylase in poly(urethane–urea) shell: inverse emulsion method

“…These advantages include, but are not limited to, easy separation, reuse, induced stabilities and potential of continuous operation (Eldin and Mita, 2014). The most popular methods of immobilisation of the enzymes or bioactive materials are their entrapment in hydrogels, for example, isopropyl acrylamide, alginate, polyionic hydrogel from chitosan and xanthan (ChitaXan), cellulose-biopolymer (Magnin et al, 2003;Kim et al, 2009;Milašinovic et al, 2010;Kim et al, 2012;Zhang et al, 2013). Immobilisation of bioactive materials in sol-gel silica matrices (Bhatia and Brinker, 2000;Wei et al, 2000;Macario et al, 2009) and encapsulation in liposomes (Nasseau et al, 2001;Huysmans et al, 2005;Hwang et al, 2012) were also applied.…”