Immobilization of methylene blue using α‐zirconium phosphate and its application within a reagentless amperometric hydrogen peroxide biosensor

Abstract

A novel H202 sensor was constructed by employing methylene blue (MB) incorporated in a-zirconium phosphate as an electron transfer mediator. Methylene blue was intercalated into a a-zirconium phosphate modified horseradish peroxidase (HRP) immobilization matrix cross-linked on a glassy carbon electrode surface via bovine serum albumin (BSA)-glutaraldehyde. Such a coimmobilization matrix of the mediator and enzyme was formed from a-zirconium phosphate-MB inclusion colloid in which horseradish peroxidase was dissolved. Intercalation of methylene blue in layered a-zirconium phosphate was investigated by X-ray power diffraction (XRD), IR spectra and electrochemical measurements. Methylene blue immobilized in this way underwent a quasi-reversible electrochemical redox reaction. Cyclic voltammetry and amperometric measurements demonstrated that methylene blue coimmobilized with HRP displayed good stability and efficiently shuttled electrons between HRP and the electrode. The sensorresponded rapidly and linearly to HzOz over the range from 2.0 x mo1L Keywords: Amperometric enzyme biosensor, a-Zirconium phosphate, Methylene blue, Hydrogen peroxide to 5 . 0~ mol/L, with a detection limit of 3.0 x

Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate

“…15 The present paper is the first report on the application of GOx immobilized in ZrP for an amperometric glucose sensor. Although Ruan et al 16 reported on a ZrP-based enzyme electrode with peroxidase and a mediator embedded in ZrP, there has been no report on glucose sensors based on GOx embedded in ZrP to our best knowledge. In this study, GOx was intercalated in ZrP according to the method described by Kumar et …”

Adsorption of a copper (II) complex on calcium phosphate intercalated with 4-aminobenzoic acid: synthesis and electrochemical investigation

“…23 Owing to these characteristics, a crystalline layered phosphate compound can be used as a matrix for immobilization of electroactive species. 24 This investigation focuses on the electrochemical behavior of copper (II) as it is adsorbed on 4-aminobenzoic acid intercalated calcium phosphate. This newly synthesized compound was first used to prepare a carbon-paste electrode.…”

Carbon-felt-based Bioelectrocatalytic Flow-detectors: Optimization of the Adsorption Conditions of Horseradish Peroxidase and Thionine onto Carbon-felt for Highly Sensitive Amperometric Determination of H2O2

“…Up to now, large numbers of peroxidase-based H2O2-sensing electrodes have been reported. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] Among various heme-containing plant peroxidases, horseradish peroxidase (HRP) has been widely employed in enzyme electrodes, because HRP is relatively stable, cost-effective and commercially available in highly purified form. In addition, the structure and the reaction mechanism of the HRP has been well investigated.…”