Immobilization of papain: A review

Tacias-Pascacio, Veymar G.; Morellon‐Sterling, Roberto; Castañeda‐Valbuena, Daniel; Berenguer-Murcia, Ángel; Kamli, Majid Rasool; Tavano, Olga Luisa; Fernández‐Lafuente, Roberto

doi:10.1016/j.ijbiomac.2021.08.016

Cited by 72 publications

(27 citation statements)

References 316 publications

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“…Bacteriocin ZFM216 is insensitive to papain. This is probably because the substrate non-specificity of papain for bacteriocin ZFM216 or the catalytic triad (Ser-His-Asp) of papain can’t act on bacteriocins ( Tacias-Pascacio et al, 2021 ). A complete description requires a knowledge of both structure of papain and bacteriocin ( Mccammon et al, 1976 ).…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of bacteriocin produced by a strain of Lacticaseibacillus rhamnosus ZFM216

Dai²,

Shi³

et al. 2022

Front. Microbiol.

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The recent surge in demand for natural preservatives has ushered in a new era of research into novel bacteriocins capable of effectively combating food-borne infections. In this study, the bacteriocin from Lacticaseibacillus rhamnosus ZFM216, which has a molecular mass of 11851.9 Da, was purified using macroporous resin, gel chromatography, and reversed-phase high performance liquid chromatography. This bacteriocin could inhibit both Gram-positive and Gram-negative bacteria. It had a strong inhibitory effect on Staphylococcus aureus D48 with minimum inhibitory concentration values of 1.75 μM. Bacteriocin ZFM216 was heat stable and showed pH stability under weakly acidic conditions. It was sensitive to pepsin, proteinase K and trypsin. Electron microscopy results showed that when treated with bacteriocin ZFM216, S. aureus D48 was severely deformed, the cell structure was obviously changed, and the intracellular electrolyte leaked to the outside of the cell. Bacteriocin ZFM216 caused the ATP level of the indicator to decrease, the conductivity to sharply increase, and the transmembrane potential difference (ΔΨ) to instantaneously decrease. This research formed the basis for further development and utilization of bacteriocin ZFM216 which has potential in the food industry.

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Section: Discussionmentioning

confidence: 99%

Purification and characterization of bacteriocin produced by a strain of Lacticaseibacillus rhamnosus ZFM216

Dai²,

Shi³

et al. 2022

Front. Microbiol.

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“…Table 15 summarizes support materials commonly used for plant protease immobilization. In general, immobilized enzymes exhibit better adaptability to alkaline conditions, reusability and higher resistance to thermal inactivation and extreme pH values than the soluble ones [4,280] . The selection of optimal support and activation methods depends on affinity to enzyme, biocompatibility, availability and price, presence of reactional functional groups, regeneration and reusability, insolubility under reaction conditions and chemical and thermal stability.…”

Section: Biotechnological Applications Of Plant Proteases: Perspectiv...mentioning

confidence: 99%

“…In general, immobilized enzymes exhibit better adaptability to alkaline conditions, reusability and higher resistance to thermal inactivation and extreme pH values than the soluble ones. [4,280] The selection of optimal support and activation methods depends on affinity to enzyme, biocompatibility, availability and price, presence of reactional functional groups, regeneration and reusability, insolubility under reaction conditions and chemical and thermal stability. The procedures for plant proteases immobilization should provide precise control of enzyme orientation to preserve the functional properties of the active site during the process.…”

Section: Biotechnological Applications Of Plant Proteases: Perspectiv...mentioning

confidence: 99%

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

2022

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An updated review of emerging plant proteases with potential biotechnological application is presented. Plant proteases show comparable or even greater performance than animal or microbial proteases for by‐product valorization through hydrolysis for, for example, cheese whey, bird feathers, collagen, keratinous materials, gelatin, fish protein, and soy protein. Active biopeptides can be obtained as high added value products, which have shown numerous beneficial effects on human health. Plant proteases can also be used for wastewater treatment. The production of new plant proteases is encouraged for the following advantages: low cost of isolation using simple procedures, remarkable stability over a wide range of operating conditions (temperature, pH, salinity, and organic solvents), substantial affinity to a broad variety of substrates, and possibility of immobilization. Vegetable proteases have enormous application potential for the valorization of industrial waste and its conversion into products with high added value through low‐cost processes.

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“…Among these, synthetic compounds of polyacrylamides are mostly used because it is more resistant than calcium chloride, but one disadvantage is that these are high cost and creates toxicity in living algae cells. Thus, algae immobilization has huge potential for removing toxic wastes in heavy metal pollution [39].…”

Section: Pretreatment Of Metal Ions Using Algae Biomassmentioning

confidence: 99%

Reduction of Heavy Metal Pollution Using Brown Algae: A Review

Sreevani¹

2022

J NanoSci Res Rep

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Industrial heavy metal pollution and other effluents from chemical industries are of great concern for the ecosystem. Due to this, toxic waste water is released into the environment causing severe heavy metal pollution and also affects our health. The physicochemical methods are expensive and these results in the incomplete removal of metal ions. As a result, other alternative methods such as algae bioremediation are taken into account which have the ability to absorb metal ions and this very idea can be used in the treatment of wastewater. They absorb metal ions on the cell surface and in the intracellular ligands. Before releasing algae into waste water, they are treated with pretreatment. Various pretreatment improves the enhancement of metal sorption in algae. The most suitable and economic method for cultivating algae biomass is the calcium chloride pretreatment. When considering algae, there are various strains and hence, selection of strain is highly important. This happens by the understanding of bio sorption mechanisms, increasing the number of surface groups with genetic manipulation of algae and creating them for economic feasibility. In brown algae, the order Laminariales and Fucale species are considered as efficient groups of algae while bio-sorption is considered, because their cell wall is very rich in extracellular polymers and polysaccharides. Considering these parameters in the significant development of low-cost algae cultivation and an eco-friendly solution for the pollution caused by chemical industries, this work concentrates on the bioremediation process for the reduction of heavy metal pollution.

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Immobilization of papain: A review

Cited by 72 publications

References 316 publications

Purification and characterization of bacteriocin produced by a strain of Lacticaseibacillus rhamnosus ZFM216

Purification and characterization of bacteriocin produced by a strain of Lacticaseibacillus rhamnosus ZFM216

Production of Plant Proteases and New Biotechnological Applications: An Updated Review

Reduction of Heavy Metal Pollution Using Brown Algae: A Review

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