Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups

Influence of Experimental Conditions during Synthesis on the Physicochemical Properties of the SPION/Hydroxyapatite Nanocomposite for Magnetic Hyperthermia Application

“…The peak recorded at 575 cm −1 represents the stretching vibration Fe-O in the crystallographic lattice of SPIONs. The following sharp peak at approximately 1625 cm −1 and the broad band at 3400 cm −1 can also be ascribed to the vibration in the Fe-O, including hydroxyl groups [34,39] such as the angular vibration of O-H and O-H stretching [40]. After coating, the peak of Fe-O was slightly shifted from 575 cm −1 to 616 cm −1 , which stands for the interaction between Fe3O4 and citric acid.…”

Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde‐modified polydopamine‐coated Fe3O4 nanoparticles

“…The synthesis of Fe 3 O 4 NPs was referenced to a literature. 28 The synthetic process was shown as follows: first, nbutanol (22.0 ml), cyclohexane (50.0 ml), OP-10 (15.0 ml), and the aqueous solutions of FeCl 2 ⋅4H 2 O (0.0528 mol/L, 12.5 ml) and FeCl 3 ⋅6H 2 O (0.0890 mol/L, 12.5 ml) were introduced into a three-necked flask sequentially under electric stirring, and then the flask was put into a 20 • C water bath. Afterward, nitrogen gas was bubbled in the flask for 30 min to fully displace the atmosphere from air to nitrogen.…”

“…In this work, a novel modified Fe 3 O 4 NPs carrier, Fe 3 O 4 @PDA‐GA NPs, used for immobilized PGA was prepared through the schemed process (Scheme 1), and the practical operation was illustrated as follows: First, Fe 3 O 4 NPs were fabricated by a reverse microemulsion method referenced to a literature 28 . PDA was coated on the surface of Fe 3 O 4 NPs through the spontaneous oxygen‐mediated self‐polymerization of DA in an aqueous solution of pH = 8.5.…”

“…26,27 S C H E M E 1 Synthetic pathway of nanocomposite particles Fe 3 O 4 @PDA-g-GA and Schematic view of immobilized PGA In this work, a novel modified Fe 3 O 4 NPs carrier, Fe 3 O 4 @PDA-GA NPs, used for immobilized PGA was prepared through the schemed process (Scheme 1), and the practical operation was illustrated as follows: First, Fe 3 O 4 NPs were fabricated by a reverse microemulsion method referenced to a literature. 28 PDA was coated on the surface of Fe 3 O 4 NPs through the spontaneous oxygenmediated self-polymerization of DA in an aqueous solution of pH = 8.5. Subsequently, GA was employed as a coupling agent to modify the PDA-coated Fe 3 O 4 NPs via a Schiff base reaction between the amino groups of PDA coating and aldehyde groups of GA. After that, the synthesized nanocomposites from Fe 3 O 4 NPs to Fe 3 O 4 @PDA-GA NPs carrier were characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM) techniques.…”

Hg2+ Significantly Enhancing the Peroxidase-Like Activity of H2TCPP/ZnS/CoS Nanoperoxidases by Inducing the Formation of Surface-Cation Defects and Application for the Sensitive and Selective Detection of Hg2+ in the Environment

“…Among these, carcinogenic mercury ions (Hg 2+ ) are dangerous to the kidney, nervous system, lungs, and heart in humans. , Previously, the common methods used to detect heavy-metal ions mainly include liquid chromatography, atomic absorption spectrometry, inductively coupled plasma mass spectrometry, and atomic fluorescence spectrometry . The determination of heavy-metal ions require some expensive and sophisticated instruments, together with complicated pretreatment procedures, which limits their wide application. , In recent years, nanozyme-based sensors have attracted extensive attention because of their advantages of simple operation, convenience, and visual observation. , Scientists have explored various nanomaterials with catalytic activity such as metal oxides, metal sulfides, semiconductor heterojunctions, − and metal–organic framework composite materials, − which have been applied in the fields of biosensors, medical detection, food, environment, etc. − In order to realize the rapid detection of heavy-metal ions and construction of an efficient colorimetric sensing platform, some nanozymes with high catalytic activity need to be explored.…”