Immobilization of penicillin G acylase on functionalized macroporous polymer beads

Bahulekar, Raman; Prabhune, Asmita; SivaRaman, H.; Ponrathnam, S.

doi:10.1016/0032-3861(93)90300-y

Cited by 33 publications

(15 citation statements)

References 7 publications

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“…Enzyme immobilization is considered as one of the most popular biotechnological applications of different synthetic polymers including PVC 1–9. Enzyme immobilization facilitates the purification of the reaction system and the recovery of enzyme and makes it possible to use the enzyme repetitively or continuously.…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilization of β‐galactosidase onto amino‐functionalized PVC microspheres

Eldin

El-Aassar

Elzatahry

et al. 2012

J of Applied Polymer Sci

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β‐Galactosidase enzyme, from Aspergillus oryzae, was covalently immobilized onto amino‐functionalized PVC microspheres after activation with gultaraldehyde. PVC microspheres were functionalized by introducing terminal amine groups via amination process with ethylene diamine (EDA). Furthermore, verifications of amination process were obtained using TGA, FT‐IR, and SEM analysis. Different factors affecting the amination process were investigated and their impact on the activity and the retention of immobilized enzyme's activity was monitored. Concentration of ethylene diamine, amination temperature, and time were found for a determined effect. Reaction with EDA (0.025%) at 30°C for 40 minutes was found for optimum conditions. Variation of PVC : EDA mass ratio over 1 : 1 was found with neglectable effect. Thermal stability of immobilized enzyme was recognized where the immobilized form kept 80% of its original activity compared with 20% for the free form. Denaturation tolerance against pH was observed where immobilized form kept 60% of its original activity after 300 minutes incubation at pH 7.0 in the absence of substrate while the free form kept only 15% of its activity under the same conditions. Moreover, immobilized form show storage stabilities where immobilized form kept 40% of its original activity after four weeks while the free form kept only 25% of its initial activity. Under optimum conditions for enzyme immobilization, 1 kg of immobilized enzyme retains 87% of its native activity and has 11,000 activity units. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012

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Section: Introductionmentioning

confidence: 99%

Covalent immobilization of β‐galactosidase onto amino‐functionalized PVC microspheres

Eldin

El-Aassar

Elzatahry

et al. 2012

J of Applied Polymer Sci

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show abstract

“…A great variety of polymer microspheres have been induced as robust biocatalyst carriers in quite a lot of industrial applications (Bahulekar et al, 1993). Polystyrene (PS) and polymethyl methacrylate (PMMA) resins, which have been widely applied in a variety of industrial processes, are the major species (Omi et al, 1997).…”

Section: Macroporous Organic Microspheresmentioning

confidence: 99%

Microspheres for Enzyme Immobilization

Gao¹,

Wang²,

Ma³

2013

Microspheres and Microcapsules in Biotechnology

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“…Currently, many types of carrier, for example natural polymer such as proteins, gelatine, and chitosan, and some synthetic polymers, for example Amberlite CG 50 [278] can be activated by dialdehyde, e.g. glutaraldehyde, to give aldehyde pendant groups.…”

Section: Polymers Containing Amino Groupsmentioning

confidence: 99%

Covalent Enzyme Immobilization

Cao¹

2005

Carrier‐bound Immobilized Enzymes

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Immobilization of penicillin G acylase on functionalized macroporous polymer beads

Cited by 33 publications

References 7 publications

Covalent immobilization of β‐galactosidase onto amino‐functionalized PVC microspheres

Covalent immobilization of β‐galactosidase onto amino‐functionalized PVC microspheres

Microspheres for Enzyme Immobilization

Covalent Enzyme Immobilization

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