Immobilization of pepsin on chitosan beads

Altun, Gamze Durgun; Çetinus, Şenay Akkuş

doi:10.1016/j.foodchem.2005.11.005

Cited by 116 publications

(91 citation statements)

References 29 publications

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“…The optimum temperature values for the native and immobilized pepsin were found in the range from 37 to 40 °C and from 40 to 50 °C, respectively. The results are similar to results described for pepsin immobilized on chitosan beads, silicone elastomers, and on an aldehyde-modified polymethacrylate monolith (Altun andCetinus, 2007, Poojari et al, 2009;Han et al, 2014), where the optimum temperature for the immobilized pepsin was higher than that for native enzyme and depended on the tested support. The increase in optimum temperature is caused by the change of the physical and chemical properties of the enzyme by the immobilization process.…”

Section: Brazilian Journal Of Chemical Engineeringsupporting

confidence: 87%

“…Operational stability for pepsin immobilized on silica-gel (carriers 5-7) and acrylic carriers (carrier 2). Altun and Cetinus (2007) observed that pepsin immobilized on chitosan beads retained 95% of the specific activity after three cycles and this activity decreased gradually during the operational process. In the case of pepsin immobilized on acrylamide/2-hydroxyethyl methacrylate copolymer the immobilized enzyme retained 55% of its activity after six cycles of application (Shukla and Devi, 2005).…”

Section: Operational Stability Of Immobilized Pepsin In a Batch Reactormentioning

confidence: 95%

“…The covalent bond formation via amino groups of the immobilized enzyme might also have reduced the conformational flexibility, as immobilized enzymes need a higher temperature to form the proper conformation to recognize and bind substrate molecule (Hu et al, 2006;Altun and Cetinus, 2007). In the case of pepsin, the immobilization process probably gave rise to increased rigidity of the tertiary structure of the enzyme, which prevented the molecule from expanding (Mateo et al, 2007).…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

“…For example, Stigter et al (2008) described the use of pepsin microreactors consisting of pepsin immobilized in a fused-silica capillary. Pepsin was also immobilized on chitosan beads (Altun and Cetinus, 2007), acrylic copolymers with methacrylates as a co-monomer (Li et al, 2004;Shukla and Devi, 2005;Hu et al, 2006), a modified alumina complex (Ticu et al, 2005), agarose beads (Kurimoto et al, 2001), silica gels (Line et al, 1971), and cellulose-based carriers (Dumitriu et al, 1987). Most researchers focused their attention on one carrier (Hu et al, 2006) or a limited number of carriers but with the same basic structure (Poojari et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Novel Application of Porous and Cellular Materials for Covalent Immobilization of Pepsin

Szałapata

Osińska-Jaroszuk

Bryjak

et al. 2016

Braz. J. Chem. Eng.

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-Pepsin was immobilized via covalent bonds on different carriers: a silica gel carrier, acrylic beads, and a cellulose-based carrier -Granocel. All carriers were functionalized through the presence of -OH, -COOH, -NH 2 , or glycidyl groups on their surfaces. Three different cross-linkers were used for activation thereof. The results showed that Granocel activated by glutaraldehyde or carbodiimide and silica gel activated by glutaraldehyde were suitable carriers for the expression of enzyme activity. The optimum pH range for the native enzyme was 2.5-3.5 and this range was extended to the value 6.5 in the case of enzyme immobilized on the silica gel carrier and on Granocel. The optimum temperature values for the native and immobilized enzyme were in the range 37-40 °C and 40-50 °C, respectively. The activity of the immobilized pepsin at different values of pH and temperature was higher in comparison with the activity of the free enzyme.

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Section: Brazilian Journal Of Chemical Engineeringsupporting

confidence: 87%

Section: Operational Stability Of Immobilized Pepsin In a Batch Reactormentioning

confidence: 95%

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Novel Application of Porous and Cellular Materials for Covalent Immobilization of Pepsin

Szałapata

Osińska-Jaroszuk

Bryjak

et al. 2016

Braz. J. Chem. Eng.

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“…Diversos polímeros sintéticos e naturais têm sido utilizados, destacando-se o poli(óxido de etileno) 25 , poli(álcool vinílico) 13,26 , carboximetilcelulose 12,13 , alginato de sódio 27 e quitosana 28 .…”

Section: Introductionunclassified

Imobilização de lipases em filme de caseinato de sódio/glicerol: aplicação na síntese de ésteres

Sebrão¹,

Silva²,

Nascimento³

et al. 2007

Quím. Nova

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Recebido em 12/7/06; aceito em 1/12/06; publicado na web em 24/7/07 LIPASE IMMOBILIZATION IN SODIUM CASEINATE/GLYCEROL FILM: APPLICATION IN ESTER SYNTHESIS. Lipases from different sources were immobilized in sodium caseinate/glycerol film and used in the esterification reactions of aliphatic acids with alcohols in the presence of organic solvents. Lipases from Pseudomonas sp and Rhizopus oryzae were selected and the influence of several parameters was analyzed, including: lipase loading, organic solvent polarity, reaction temperature, chain length of alcohol and acid and enzyme/support reuse. For comparison, free enzymes were used under similar experimental conditions. Keywords: sodium caseinate/glycerol; lipases; immobilization. INTRODUÇÃOAs enzimas apresentam várias propriedades que as tornam atrativas como catalisadores para biotransformações, destacando-se o uso na indústria químico-farmacêutica. Estas proteínas são catalisadores versáteis, existindo em geral um processo enzimático equivalente para cada tipo de reação orgânica [1][2][3] .A partir da década 80 tem sido observado um interesse crescente no desenvolvimento de técnicas de imobilização de enzimas e/ou microrganismos, visando minimizar os efeitos causados pelo seu uso em ambientes adversos, tais como solventes orgânicos, variações no pH e/ou altas temperaturas 1,4 . A imobilização do biocatalisador em um suporte, sem prejuízo de sua atividade por um período razoável de tempo, pode assegurar sua repetida utilização ou mesmo o uso em reatores contínuos, resultando em economia nos processos industriais. Assim, de modo geral, a utilização de materiais imobilizados além de diminuir o custo por análise, aumenta a rapidez e a exatidão do processo [4][5][6] .Um dos fatores que sempre deve ser considerado na utilização de um determinado sistema de imobilização é o tipo de interação entre o suporte e o biocatalisador, que pode influenciar diretamente na estabilidade e nos efeitos cinéticos da catálise 1,4-9 . Os processos de imobilização podem ocorrer por adsorção ou ligação da enzima em um material insolúvel, pelo uso de um reagente multifuncional através de ligações cruzadas 10,11 , por confinamento em matrizes formadas por géis poliméricos 12,13 , por encapsulação em membranas poliméricas 12,14 e em micelas reversas 15,16 .Outra técnica também utilizada, apesar de não manter uma barreira física entre a enzima e o meio reacional, é o sistema bi ou trifásico. Pesquisas recentes têm demonstrado a utilização de novos materiais para imobilização de enzimas, destacando-se os líquidos iônicos com formação de sistemas trifásicos 17-21 , as nanopartículas de sílica funcionalizada e nanocompósitos de sílica-ouro [22][23][24] .Duas das técnicas de imobilização mais utilizadas são, provavelmente, as de inclusão ou microencapsulação através do confinamento dos biocatalisadores em polímeros insolúveis (formando filmes), ou em microcápsulas. Uma das principais vantagens da utilização destas técnicas é que a enzima não interage quimicamente com o polímero, evitando a des...

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Interconnection Between Biotechnology and Textile: A New Horizon of Sustainable Technology

Mallick¹

2018

Handbook of Renewable Materials for Coloration and Finishing

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Immobilization of pepsin on chitosan beads

Cited by 116 publications

References 29 publications

Novel Application of Porous and Cellular Materials for Covalent Immobilization of Pepsin

Novel Application of Porous and Cellular Materials for Covalent Immobilization of Pepsin

Imobilização de lipases em filme de caseinato de sódio/glicerol: aplicação na síntese de ésteres

Interconnection Between Biotechnology and Textile: A New Horizon of Sustainable Technology

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