Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

Zhan, Jianfeng; Jiang, Shaotong; Pan, Lijun

doi:10.1590/s0104-66322013000400004

Cited by 32 publications

(11 citation statements)

References 27 publications

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“…These results were possibly due to the porosity of the prepared beads. A high concentration of PVOH and the modified xylan might lead to the high density of physical cross-linking sites, resulting in the formation of small pores on and inside the beads, which probably limits the diffusivity of the substrate (Zhan et al 2013). In contrast, the low concentration of the modified xylan and PVOH may cause a reduction in mechanical strength and the increment of the pore size resulting in enzyme leakage (El-Tanash et al 2011).…”

Section: Optimization For Laccase Immobilization Into the Modified Xymentioning

confidence: 99%

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Bankeeree¹,

Prasongsuk²,

Imai³

et al. 2016

BioResources

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In an attempt to find a more efficient technique for biodegradation of the recalcitrant Reactive Black 5 (RB-5) dye, a composite xylan-polyvinyl alcohol (xylan-PVOH) hydrogel was used to immobilize laccase from the white-rot fungus Trametes versicolor. Xylan was prepared from the black liquor of pulp and paper effluent, and it was esterified with citric acid prior to cross-linking with polyvinyl alcohol (PVOH). The optimum composition for the immobilized laccase bead formation consisted of 4% (w/v) modified xylan, 10% (w/v) PVOH, and 15 U.mL -1 crude laccase. The maximum decolorization of RB-5 (98.45  1.96 %) was obtained within the first cycle (6 h) at 40 °C. In the eighth cycle, the reused beads were able to decolorize 55.35  2.46 % of the RB-5. Moreover, the xylan-PVOH beads extended the optimum pH range of laccase activity from 6 to 10 and tolerated a temperature up to 10 °C higher than that of the free enzyme. These results suggest that the xylan-PVOH bead has great potential as the polymer matrix for enzyme immobilization, which has applications in wastewater treatment.

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Section: Optimization For Laccase Immobilization Into the Modified Xymentioning

confidence: 99%

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Bankeeree¹,

Prasongsuk²,

Imai³

et al. 2016

BioResources

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“…PVA effectively improves the stability of immobilized cell beads with a promoted mechanical strength, while SA improves the surface properties of immobilized cell beads to keep from the tendency to agglomeration. Therefore, SA-PVA hydrogel can be regarded as a proper material for cell immobilization (Zhan et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production

Wang

Huang

Laffend

et al. 2020

Bioresour. Bioprocess.

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Parametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box-Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/ (L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process.

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“…In recent studies by Singh et al [6], synthetic organic carriers have gained a lot of attention mainly due to their effectiveness in protection from harsh environments such as high temperature or pressure. Furthermore, Zhan et al [7] indicated that sodium alginate-PVA based matrices boast excellent properties for parameters such as reusability and stability. PVA contributes to the improvement of durability, whereas sodium alginate is said to improve surface properties such as diffusibility and porosity, which in turn increases interaction.…”

Section: Resultsmentioning

confidence: 99%

Novel immobilization techniques of Acinetobacter (V2) and Paenibacillus (D9) bacterial strains for waste oil degradation

Jeon

Bissessur

Singh

2019

Biotechnology & Biotechnological Equipment

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Bacterial isolates used for the degradation of waste oils has gained greater interest in recent times since these isolates can be immobilized. However, it's their lack of reproducibility and continual usage that hinders its applicability. The aim of this work is to identify, purify, cultivate and immobilize bacterial isolates that are potential candidates to effectively and selectively degrade waste oils and waste oil blends and reuse them. In an attempt to render bacterium isolates applicable to degradation of lubricating and insulating oil in industrial processes this work investigates the immobilization (through support material, glutaldehyde activated amberlite, polyvinyl alcohol-sodium alginate and chitosan-sodium alginate matrix beads) of bacterial isolates. The data and observed trends show that developed immobilization techniques were successful in immobilizing bacterial isolate strains V2 and D9, with free cell degradation being most efficient (14.7-31.6%) followed by polyvinyl alcohol-sodium alginate (5.3-26.7%), chitosansodium alginate (8.8-0.3%) and amberlite-glutaldehyde (2.2-7.0%). The entrapment technique for immobilization of polyvinyl alcohol-sodium alginate and chitosan-sodium alginate proved to be more efficient than adsorption technique used for immobilization of amberlite-glutaldehyde. Free cell degradation was effective in degrading waste oil, but did so upon a single cycle with deterioration. Polyvinyl alcohol-sodium alginate bead matrix can be applied for degradative processes over a wide pH range and short incubation times while chitosan-sodium alginate is best suited for continuous long incubation periods. Reusability of both polyvinyl alcohol-sodium alginate and chitosan-sodium alginate beads are applicable up to a 10-cycle period.

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Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

Cited by 32 publications

References 27 publications

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production

Novel immobilization techniques of Acinetobacter (V2) and Paenibacillus (D9) bacterial strains for waste oil degradation

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