Immobilization of photochemically‐active chloroplasts onto diethylaminoethyl‐cellulose

Shioi, Yuzo; Sasa, Tsutomu

doi:10.1016/0014-5793(79)81032-7

Cited by 22 publications

(2 citation statements)

References 15 publications

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“…To address the problem of the weak functional stability of isolated photosynthetic materials, a wide range of immobilization techniques have recently been developed. Chloroplasts or thylakoid membranes were immobilized by microencapsulation within a permeable membrane (Kitajima and Butler,19T6), by adsorption onto cellulose derivatives (Shioi and Sasa, 1979), by gel entrapment (Gisby and Hall, 1980;Karube et al, 1981;Ochiai et al, 1982), or by cross-linking in a protein matrix (Fujimura et al, 1981;Isaakidou and Papageorgiou, 1979;Thomasset et al, 1982). The immobilization in an albumin-glutaraldehyde cross-linked matrix provided better stability of biological functions when compared to several other techniques (Cocquempot et al, 1981;Thomasset et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Properties of Immobilized Thylakoid Membranes in a Photosynthetic Photoelectrochemical Cell

Lemieux

Carpentier

1988

Photochem & Photobiology

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Abstract— Thylakoid membranes isolated from spinach were immobilized in a cross‐linked albumin‐glutaraldehyde matrix. Their properties in a single‐compartment photoelectrochemical cell using platinum electrodes in potentiostatic mode were compared with the native material. The porous network of the immobilized tissue retained a relatively significant quantity of aqueous media which could be modified at will by aspiration of the proper media. In the presence of the acceptor potassium ferricyanide the photocurrent generation was enhanced up to a value of one hundred times higher than the maximum output of thylakoids entrapped in a polyvinyl alcohol film deposited on a Sn02 electrode. Immobilization in the albumin‐glutaraldehyde matrix resulted in a better resistance to high pH, elevated temperatures and continuous exposure to high light intensity in potentiostatic mode (working conditions) in the electrochemical cell.

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Section: Introductionmentioning

confidence: 99%

Properties of Immobilized Thylakoid Membranes in a Photosynthetic Photoelectrochemical Cell

Lemieux

Carpentier

1988

Photochem & Photobiology

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“…Recent advances have been made in techniques for immobilization of biocatalysts (27), and chloroplasts isolated from plants were immobilized by several different methods like microencapsulation (22), adsorption (33), entrapment within gels (9,14,21,29), radiation polymerization (13), or cross-linking with glutaraldehyde in the presence of albumin (9,20,23). A functional stabilization of the photosystems activities has been obtained and some of these methods were recently compared (9).…”

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confidence: 99%

Immobilized Thylakoids in a Cross-Linked Albumin Matrix

Thomasset¹,

Thomasset²,

Vejux³

et al. 1982

Plant Physiol.

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Immobilization of lettuce (Lactuca sativa) thylakoids has been performed by using glutaraldehyde and bovine serum albumin. Confiming previous reports, a stabilization of the 02 evolution activity of the photosystem II (PSII) under storage and functional conditions has been observed. The present work is devoted to the role played by mono-and divalent cations, during the immobilization process itself, on the 02 productionL Four types of measurements have been employed: kinetic measurements, low temperature (77 K) fluorescence emission, photoacoustic (PA) spectroscopy, and electron microscopy observations. We show that the effect of glutaraldehyde is complex because it acts as an inhibitor, a stabilizing agent, and a cross-linking reactive. In the present studies, the thylakoids are immobilized within a polymeric insoluble albumin matrix. The highest activity yield and the best storage conditions are obtained when 0.15 mM Na' (or K+), 1 mm Mg2+, and 0.1 mM Mn2l are present in the resuspending media before the immobilization. Due to modifications of the ionic content during such a process, structural differences are observed on the stacking degree of thylakoids. No modification of the fluorescence and PA spectra after the immobilization are found. Furthermore, a correlation between activities and spectral changes have been shown: when the activities increase, the F735 to F695 ratio increases and the PA676 to PA4o ratio decreases.In recent years, there has been progress (3,16,23,32) in the research on technological applications of photobiological solar energy conversion (biophotolysis of water, photohydrogen production, and ATP regeneration). However, the life time of the isolated chloroplasts was very short, and the stability of PSI and PSII activities over a long period of time was a crucial limitation for these applications.Recent advances have been made in techniques for immobilization of biocatalysts (27), and chloroplasts isolated from plants were immobilized by several different methods like microencapsulation (22), adsorption (33), entrapment within gels (9,14,21,29), radiation polymerization (13), or cross-linking with glutaraldehyde in the presence of albumin (9,20,23). A functional stabilization of the photosystems activities has been obtained and some of these methods were recently compared (9). In the present paper, we have selected the cross-linking technique to study the effect of mono-and divalent cations on the PSII stability.The action of glutaraldehyde on the structure and function of chloroplast has been studied extensively (17, 18, 38) and was reviewed recently by Papageorgiou (30). The glutaraldehyde fixation stabilizes activities but blocks chloroplast reactions at specific sites (17). However, some microconformational changes may still occur after fixation (18,30). In such studies, the glutaraldehyde treatment does not give insoluble and polymeric structures. In our work, the co-crosslinking method gives thylakoids immobilized within an insoluble albumin matrix. Our aim is to obtain in...

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Immobilisierung mikrobieller Zellen und deren Nutzung zur Substratwandlung – Eine Literaturstudie. 1. Fortsetzung

Berger¹

1982

Acta Biotechnologica

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Immobilization of photochemically‐active chloroplasts onto diethylaminoethyl‐cellulose

Cited by 22 publications

References 15 publications

Properties of Immobilized Thylakoid Membranes in a Photosynthetic Photoelectrochemical Cell

Properties of Immobilized Thylakoid Membranes in a Photosynthetic Photoelectrochemical Cell

Immobilized Thylakoids in a Cross-Linked Albumin Matrix

Immobilisierung mikrobieller Zellen und deren Nutzung zur Substratwandlung – Eine Literaturstudie. 1. Fortsetzung

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