Immobilization of polyphenol oxidase on chitosan-coated polysulphone capillary membranes for improved phenolic effluent bioremediation

The influence of nano-sized TiO2 fillers on the morphologies and properties of PSF UF membrane

“…Many studies of modification of PSF membranes have been made to enhance their hydrophilicity. These studies can be divided into three routes: (1) blending with hydrophilic materials, such as blending with polyvinylpyrrolidone (PVP) [14,15], or with some minerals including silica and zirconium dioxide (ZrO 2 ) [16,17]; (2) grafting with hydrophilic polymers, monomers or functional groups [18][19][20][21]; (3) coating with hydrophilic polymers [22]. Among these methods, blending with inorganic materials has a much interest due to their convenient operations, mild conditions, and good and stable performances.…”

Binary immobilization of tyrosinase by using alginate gel beads and poly(acrylamide-co-acrylic acid) hydrogels

“…It is well known that tyrosinase catalyzes two different oxygen-dependent reactions that occur consequently: the o-hydroxylation of monophenols to yield o-diphenols (cresolase activity) and the subsequent oxidation of o-diphenols to o-quinones (catecholase activity). The enzyme has been proposed for synthesis of 3,4-dihydroxyphenylalanine (l-DOPA) [3], dephenolization of industrial wastewater [4][5][6][7], as a part of an enzyme electrode for the determination of phenol and its derivatives [8,9], bioremediation of contaminated soils [10,11], and fruit juice clarification [12], thus it is an industrially important biomolecule. The production of l-DOPA from l-tyrosine is possible by using immobilized tyrosinase [3,13].…”

Immobilization of tyrosinase on chitosan–clay composite beads

“…Conventional methods such as solvent extraction, deep-well injection and microbial degradation are used for dephenolization of waste waters [2][3][4]. Enzymes are also used for alternative dephenolization technology and tyrosinase, one of the enzymes which is suitable for treatment of phenolic wastes [5,6]. Tyrosinase or polyphenol oxidase (EC 1.14.18.1; monophenol monooxygenase) is a copper enzyme which catalyzes the hydroxylation of monophenols to o-diphenols and the oxidation of the latter to o-quinones, using oxygen [7].…”