Immobilization of protein ligands with methyl vinyl ether-maleic anhydride copolymer

Immobilization of Saccharides and Peptides on 96-Well Microtiter Plates Coated with Methyl Vinyl Ether–Maleic Anhydride Copolymer

“…The procedures are according to the method previously reported (1). One milligram of MMAC dissolved in 200 l of dimethyl sulfoxide (DMSO) was added to each well of the microtiter plate (Immulon I, Dynatech Labs., Chantilly, VA) and allowed to stand for 30 min.…”

“…Isosaki et al have reported the successful use of methyl vinyl ether-maleic anhydride copolymer (MMAC) for immobilization of protein ligands (1). MMAC is a waterinsoluble polymer with active acid anhydride groups that react with the amino groups of ligands to form stable covalent amide bonds.…”

Bioadhesive properties of pegylated nanoparticles

“…Thus, in an aqueous environment, the anhydride group is opened yielding two carboxylic acid groups. This reaction involves the dissolution of the copolymer in water [39]. However, the presence of molecules with active groups may permit the binding of ligands (i.e., PEG) to the copolymer backbone or to the surface of the just-formed NPs [38].…”

Covalent Enzyme Immobilization

“…• Enzymes can also be immobilized on ethylene-maleic anhydride copolymers (EMA) [262] with well defined and controlled anhydride content, achieved by controlled hydrolysis. The optimum recovery of immobilized enzyme activity and of insoluble material was achieved with EMA samples containing 50-70 % anhydride [155].…”