Immobilization of Proteins and Enzymes, Mesoporous Supports

Hartmann, Martin; Jung, Dirk

doi:10.1002/9780470054581.eib364

Cited by 2 publications

(3 citation statements)

References 254 publications

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“…According to the IUPAC definition, mesoporous materials are materials that possess nanometer pores in the size range of 2−50 nm. 3 This size range matches the dimension of different biological entities such as proteins, cell membranes, and lipids. Mesoporous materials can thus serve as favorable hosts that provide nanoscale environments for confinement of biomolecules for various purposes.…”

Section: Introductionmentioning

confidence: 86%

“…Structural and functional characterization of biomolecules and/or their ensembles confined in specific nanoscale environments could offer new clues to fundamental understanding of complex biological events, where the use of mesoporous materials has emerged as a powerful tool. According to the IUPAC definition, mesoporous materials are materials that possess nanometer pores in the size range of 2–50 nm . This size range matches the dimension of different biological entities such as proteins, cell membranes, and lipids.…”

Section: Introductionmentioning

confidence: 99%

“…42 Briefly, silicon wafers were cut into slides with a width of 12.5 mm. These slides were cleaned by immersion for 5 min at 80°C first in NH 3 The adsorption experiments were performed using 3 mL of 25 mM Tris buffer (pH 8.5) in a cuvette while stirring at around 100 rpm. The temperature of the cuvette was maintained at 25°C.…”

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confidence: 99%

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Nanoscale Confinement and Fluorescence Effects of Bacterial Light Harvesting Complex LH2 in Mesoporous Silicas

et al. 2013

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Many key chemical and biochemical reactions, particularly in living cells, take place in confined space at the mesoscopic scale. Toward understanding of physicochemical nature of biomacromolecules confined in nanoscale space, in this work we have elucidated fluorescence effects of a light harvesting complex LH2 in nanoscale chemical environments. Mesoporous silicas (SBA-15 family) with different shapes and pore sizes were synthesized and used to create nanoscale biomimetic environments for molecular confinement of LH2. A combination of UV−vis absorption, wide-field fluorescence microscopy, and in situ ellipsometry supports that the LH2 complexes are located inside the silica nanopores. Systematic fluorescence effects were observed and depend on degree of space confinement. In particular, the temperature dependence of the steady-state fluorescence spectra was analyzed in detail using condensed matter band shape theories. Systematic electronicvibrational coupling differences in the LH2 transitions between the free and confined states are found, most likely responsible for the fluorescence effects experimentally observed.

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Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Nanoscale Confinement and Fluorescence Effects of Bacterial Light Harvesting Complex LH2 in Mesoporous Silicas

et al. 2013

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Drug delivery from structured porous inorganic materials

Arruebo

2011

WIREs Nanomed Nanobiotechnol

164

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Structured porous inorganic materials show high chemical and mechanical stability under an array of physiological conditions. Their hydrophilic character and porous structure can in principle be tailored to control the diffusion rate of an adsorbed or encapsulated drug, gene, or protein. This organized porosity has been used to achieve a sustained, controlled, or pulsed release in drug delivery applications. Their large surface areas together with their large pore volumes have been used to improve the solubility of poorly soluble drugs. Their low density allows them to float in the gastrointestinal tract and prolong the gastric retention of oral drugs. In addition, their easy surface functionalization allows their grafting with bioadhesive and targeting moieties, and their interior pore volume protects biological payloads from physiological degradation. Some of those porous inorganic materials can be synthesized or microfabricated to form deposits thus acting as drug reservoirs. Finally, diffusion-controlling porous membranes or coatings of those materials can be tailored with specific pore sizes to control drug release in eluting devices. Current research is focused on designing on demand targeted drug delivery systems using those inorganic porous materials as reservoirs together with triggering systems on their pore entrances to be externally activated to release the encapsulated therapeutic moiety. All of the previous scenarios will be overviewed to demonstrate the numerous possibilities of structured porous inorganic materials in drug delivery applications.

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Immobilization of Proteins and Enzymes, Mesoporous Supports

Cited by 2 publications

References 254 publications

Nanoscale Confinement and Fluorescence Effects of Bacterial Light Harvesting Complex LH2 in Mesoporous Silicas

Nanoscale Confinement and Fluorescence Effects of Bacterial Light Harvesting Complex LH2 in Mesoporous Silicas

Drug delivery from structured porous inorganic materials

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