Immobilization of rat brain acetylcholinesterase on porous gold-nanoparticle–CaCO3 hybrid material modified Au electrode for detection of organophosphorous insecticides

Chauhan, Nidhi; Narang, Jagriti; Pundir, C.S.

doi:10.1016/j.ijbiomac.2011.08.006

Cited by 53 publications

(23 citation statements)

References 41 publications

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“…5d). This phenomenon was also observed by others [29][30][31]. The cathodic peak might be attributed to the reduction of the thiocholine and the mechanism is currently being investigated.…”

Section: Cyclic Voltammetric Behaviors Of the Ache-mwcnt-ca-npg Electsupporting

confidence: 77%

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Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Ding

Zhang

Jia

et al. 2014

Sensors and Actuators B: Chemical

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a b s t r a c tAn electrochemical sensing platform based on assembly of carbon nanotubes on a nanoporous gold electrode is described for highly sensitive detection of organophosphate pesticides. The nanoporous gold film (NPG) electrode is fabricated by an alloying/dealloying process, which possess high electroactive surface area and is an excellent substrate for sensor design. The NPG functionalized with cysteamine allows the immobilization of carbon nanotubes on the electrode with the self-assembly technique. The carboxylated carbon nanotubes are further linkered with acetylcholinesterase (AChE) for amperometric sensing of pesticides. The immobilized AChE, as a model, shows excellent activity to its substrate and allows a quantitative measurement of organophosphate pesticides. Under the optimal experimental conditions, the inhibition of malathion is proportional to its concentration in the range of 0.001-0.5 g mL −1 with a detection limit of 0.5 ng mL −1 . The proposed method shows good reproducibility and high stability, which provides a new avenue for electrochemical biosensor design.

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“…5d). This phenomenon was also observed by others [29][30][31]. The cathodic peak might be attributed to the reduction of the thiocholine and the mechanism is currently being investigated.…”

Section: Cyclic Voltammetric Behaviors Of the Ache-mwcnt-ca-npg Electsupporting

confidence: 77%

“…Recently, a number of AChE biosensors have been developed for malathion. However, the detection limits were calculated in different ways [29,36,37]. Our reported value is much lower or comparable to those in the references [30,38].…”

Section: Calibration Curve For Malathion Determinationsupporting

confidence: 70%

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Ding

Zhang

Jia

et al. 2014

Sensors and Actuators B: Chemical

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“…Nevertheless, they are time consuming and laborious, and require expensive equipments and highly trained technicians. [9] As a good alternative and with the improvement of being quick and consistent, several bioanalytical techniques based on inhibition of acetylcholinesterase (AChE) and coupled with simple detectors have been developed recently. [10,11] Biosensors are considered a viable alternative to the chromatographic methods for pesticide determination for an on-site analysis.…”

Section: Introductionmentioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

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A new electrochemical sensing device was constructed for determination of pesticides. In this report, acetylcholinesterase was bioconjugated onto hybrid nanocomposite, i.e. iron oxide nanoparticles and poly(indole-5-carboxylic acid) (Fe 3 O 4 NPs/Pin5COOH) was deposited electrochemically on glassy carbon electrode. Fe 3 O 4 NPs was showed as an amplified sensing interface at lower voltage which makes the sensor more sensitive and specific. The enzyme inhibition by pesticides was detected within concentrations ranges between 0.1À60 and 1.5À70 nM for malathion and chlorpyrifos, respectively, under optimal experimental conditions (sodium phosphate buffer, pH 7.0 and 25 C). Biosensor determined the pesticides level in water samples (spiked) with satisfactory accuracy (96%À100%). Sensor showed good storage stability and retained 50% of its initial activity within 70 days at 4 C.

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“…The detection limits and linear ranges obtained in this work were similar to, or better than, many reported in the literature using other sensors. 10,12,[30][31][32][33][34][35][36][37][38][39][40][41][42][43] This can be attributed to the efficiency of electron transfer between the CoTSPc/CNT/OMIM[BF4]/AChE biosensor and TCh, due to the catalytic effect of the CoTSPc/CNT/OMIM[BF4] composite. In addition, the reaction occurring at the CoTSPc/ CNT/OMIM[BF4]/AChE biosensor very quickly reached a dynamic equilibrium after each addition of the analyte, with a response time of less than 1 s for the signal to reach 100%.…”

Section: Study Of the Incubation Time Of The Cotspc/cnt/omim[bf4]/ Acmentioning

confidence: 99%

Ultrasensitive Biosensor for Detection of Organophosphorus Pesticides Based on a Macrocycle Complex/Carbon Nanotubes Composite and 1-Methyl-3-octylimidazolium Tetrafluoroborate as Binder Compound

et al. 2015

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This work describes the highly sensitive detection of organophosphorus pesticides employing the cobalt(II) 4,4,4,4-tetrasulfo-phthalocyanine (CoTSPc) macrocycle complex, carbon nanotubes (CNT), and 1-methyl-3-octylimidazolium tetrafluoroborate (OMIM[BF4]). The technique is based on enzyme acetylcholinesterase (AChE) inhibition. The composite was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, and amperometry. The AChE was immobilized on the composite electrode surface by cross-linking with glutaraldehyde and chitosan. The synergistic action of the CoTSPc/CNT/OMIM[BF4] composite showed excellent electrocatalytic activity, with a low applied potential for the amperometric detection of thiocholine (TCh)

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Immobilization of rat brain acetylcholinesterase on porous gold-nanoparticle–CaCO3 hybrid material modified Au electrode for detection of organophosphorous insecticides

Cited by 53 publications

References 41 publications

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Ultrasensitive Biosensor for Detection of Organophosphorus Pesticides Based on a Macrocycle Complex/Carbon Nanotubes Composite and 1-Methyl-3-octylimidazolium Tetrafluoroborate as Binder Compound

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