Immobilization of Thrombin to Alginate Gel and in vitro Application of Immobilized Thrombin

Akkaya, Alper

doi:10.15671/hjbc.2017.169

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…This viscosity increase is likely due to the thrombin induced non-covalent supramolecular interactions, such as the ionic interaction between negatively charged carboxylate groups of sodium alginate and positively charged thrombin. [39] As expected, the fibrin zero-shear viscosity was substantially higher than that of fibrinogen, 16.9 ± 3.3 versus 3.9 ± 0.8 Pa s, respectively (Figure 3B).…”

Section: Assessment Of Bioprinting Conditionssupporting

confidence: 78%

“…The presence of thrombin increased the zero‐shear viscosity of the PEG–alginate prepolymer solution, which was 20.5 ± 6.4 Pa s as compared to the bath without the enzyme (11.7 ± 1.9 Pa s). This viscosity increase is likely due to the thrombin induced non‐covalent supramolecular interactions, such as the ionic interaction between negatively charged carboxylate groups of sodium alginate and positively charged thrombin . As expected, the fibrin zero‐shear viscosity was substantially higher than that of fibrinogen, 16.9 ± 3.3 versus 3.9 ± 0.8 Pa s, respectively (Figure B).…”

Section: Resultssupporting

confidence: 55%

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3D Printed Cartilage‐Like Tissue Constructs with Spatially Controlled Mechanical Properties

Melo

Jodat

Mehrotra

et al. 2019

Adv Funct Materials

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Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is fabricated using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a bath composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel (MPa order compressive modulus) is developed as an extracellular matrix (ECM) with self-healing properties. Within this bath supplemented with thrombin, human mesenchymal stem cell (hMSC) spheroids embedded in fibrinogen are 3D bioprinted, creating a soft microenvironment composed of fibrin (kPa order compressive modulus) that simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids present high viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to locally bioprint a soft and cell stimulating biomaterial inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro-and macromechanical properties of the 3D printed tissues such as cartilage.

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Section: Assessment Of Bioprinting Conditionssupporting

confidence: 78%

Section: Resultssupporting

confidence: 55%