2018
DOI: 10.1002/btpr.2670
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Immobilization of tobacco etch virus (TEV) protease on a high surface area protein nanofibril scaffold

Abstract: Tobacco etch virus (TEV) protease is widely used for the removal of poly-histidine affinity tags from proteins. In solution, it is a one-time use enzyme for tag cleavage that has low stability, and is therefore a good candidate for immobilization. Amyloid fibrils can act as a versatile nanoscaffold by providing a large surface area for biomolecule immobilization. Immobilization of TEV protease to amyloid fibrils grown from the surface of a small glass bead, using physisorption, successfully immobilized active … Show more

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Cited by 4 publications
(3 citation statements)
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“…However, despite the potential advantages of TEVp immobilization, there are currently no commercially available preparations of immobilized TEVp. Previously reported immobilization strategies for TEVp include the creation of a TEV-Streptag fusion and subsequent immobilization on Strep-Tactin-coated agarose beads [8], and a unique strategy for the deposition of TEVp onto amyloid fiber coated glass beads [9]. While both of these strategies resulted in immobilized TEV with retention of activity and specificity, both exhibit loss of activity with repeated use, illustrating a key challenge encountered by many enzyme immobilization strategies.…”
Section: Introductionmentioning
confidence: 99%
“…However, despite the potential advantages of TEVp immobilization, there are currently no commercially available preparations of immobilized TEVp. Previously reported immobilization strategies for TEVp include the creation of a TEV-Streptag fusion and subsequent immobilization on Strep-Tactin-coated agarose beads [8], and a unique strategy for the deposition of TEVp onto amyloid fiber coated glass beads [9]. While both of these strategies resulted in immobilized TEV with retention of activity and specificity, both exhibit loss of activity with repeated use, illustrating a key challenge encountered by many enzyme immobilization strategies.…”
Section: Introductionmentioning
confidence: 99%
“…In such conditions, the activity of growth inhibitory molecules in the lesions' site, lack of suitable growth factors, degeneration of distal proteins, the breakdown of the cytoskeleton, dissolution of the cell membrane, and swelling of the nerve stump leads to the formation of scar tissue that inhibits repair. [5][6][7] Implantation of biodegradable scaffolds helps to improve cellular proliferation and prevent the formation of scar tissue in the defective site as well as improve regeneration of neural defects. 3 For many years, autologous sources were the best solution for restoration of small diameter neural tissue lesions due to biocompatibility, lack of toxicity, transmission of disease, and stimulation of cell growth.…”
Section: Introductionmentioning
confidence: 99%
“…4 Therefore, scientists tend to use synthetic scaffolds for simulation of extracellular matrix (ECM). [5][6][7] Implantation of biodegradable scaffolds helps to improve cellular proliferation and prevent the formation of scar tissue in the defective site as well as improve regeneration of neural defects. 8,9 The scaffold microstructure is one of the key factors in guiding cellular growth and efficient regeneration.…”
Section: Introductionmentioning
confidence: 99%