Immobilization of trypsin on alginic acid–poly (glycidyl methacrylate) graft copolymer

Abstract

Trypsin was immobilized onto alginic acid-polyfglycidyl methacrylate) graft copolymer (AAGMA). The resulting immobilized enzyme showed 65% of the soluble enzymatic activity. The temperature optimum was shifted by 5°C to a higher value. The pH optimum of immobilized enzyme has also been shifted by 0.5 units toward the alkaline side when compared to that of soluble enzyme.The pH stability and thermal stability are better than that of soluble enzyme.

Novel alginate–acrylic polymers as a platform for drug delivery

“…The reported data refer either to a template-type polymerization 10,11 or to a true chemical grafting process. 12,13 In their simplest design, oral controlled-release dosage forms made from alginates are monolithic tablets in which the drug is homogeneously dispersed. Drug release is controlled by the formation of a hydrated viscous layer around the tablet, which acts as a diffusional barrier to drug diffusion and water penetration.…”