Immobilization of urease on vapour phase stain etched porous silicon

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

“…Polyethyleneimine is used as a crosslinking agent by many research teams around the world [59][60][61][62]. An example of the use of PEI to activate a substrate for immobilisation is the system using urease to detect urea [63] or peroxidase to identify paracetamol [64]. The present results show that PEI can be successfully used for the activation of chitosan microspheres with the maintenance of the enzymatic activity of the immobilised biocatalyst.…”

Electric‐Field‐Assisted Protein Transport, Capture, and Interferometric Sensing in Carbonized Porous Silicon Films

“…An important requirement of many bioassay or drug delivery applications is that the collection, concentration, and immobilization processes not denature or otherwise deactivate the biomolecule of interest. While it has been demonstrated that porous Si matrices can release antibodies,29 enzymes,19, 30–36 or other biomolecules37 in their active form, some compositions of porous Si are known to undergo irreversible chemical reactions with drugs or other molecules 38–40. In the present case, the enzymatic activity of lysozyme provides a functional assay of the compatibility of the carbonized pSi opto‐electrochemical device with sensitive proteins.…”

Porous Silicon Formation by HNO3/HF Vapor Etching

“…• HNO 3 /HF vapor etching can be advantageous for biosensor fabrication due to reduction in cost and easy scalability (large-area PS layers can be easily fabricated) (Maia et al 2007). Fig.…”