Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization

Popović, Nikolina; Pržulj, Dunja; Mladenović, Maja; Prodanović, Olivera; Ece, Selin; Đurđić, Karla Ilić; Ostafe, Raluca; Fischer, Rainer; Prodanović, Radivoje

doi:10.1016/j.ijbiomac.2021.04.115

Cited by 36 publications

(37 citation statements)

References 35 publications

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“…These results confirm that by combining laccase with the magnetite nanoparticles and with a permanent magnetic field, the percentage of dye decolorization increased compared to only the use of magnetite or Laccase-magnetite nanoparticles in the filter. This behavior has been previously described for systems where immobilized laccase enzyme from Streptomyces cyaneus was applied for laccase-coated yeast cell walls and its efficiency vs. a native polymer [13], and for systems where immobilized lipase enzyme from Candida rugosa was applied for the lipolytic activity on gold nanoparticle surfaces [44].…”

Section: Discussionmentioning

confidence: 74%

“…Several functionalization and immobilization strategies have been proposed to improve enzyme stability and resistance to environmental changes, such as the use of surface fillers, and polymers, among others [13,14]. Pertaining to immobilization processes for surface coverage, protein-protein interactions (e.g., electrostatic forces, hydrogen bonding) are favored over surface-protein interactions (e.g., bonding, chemical or physical adsorption) and the appropriate immobilization method will be chosen depending on conditions, such as pH, temperature, reaction time, among others [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Novel Magnetic Polymeric Filters with Laccase-Based Nanoparticles for Improving Congo Red Decolorization in Bioreactors

2022

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In this work, five different magnetic biofilters, containing magnetic nanoparticles (142 nm), immobilized laccase on nanoparticles (190 nm) and permanent magnetic elements, such as neodymium magnets and metallic meshes, were designed, manufactured and tested. The five types of filters were compared by measuring the decolorization of Congo Red dye inside bioreactors, the half-life of the filters and the amount of magnetic nanoparticle and enzyme lost during multiple cycles of operation. Filters containing laccase immobilized on magnetite (Laccase-magnetite), permanent magnets and metallic mesh presented the highest Congo Red decolorization (27%) and the largest half-life among all types of filters (seven cycles). The overall dye decolorization efficiencies were 5%, 13%, 17%, 23%, and 27% for the paper filter, paper filter with magnetite, paper filter with Laccase-magnetite, paper filter with Laccase-magnetite with magnets and paper filter with Laccase-magnetite with magnets and metallic mesh, respectively. Although the highest losses of magnetite occurred when using the filters containing magnets (57 mg), the use of permanent magnetic elements in the filters increased the half-life of the filter three-fold compared to the filters without enzymatic properties and two-fold compared to the filters with Laccase-magnetite. Results indicate that the novel use of permanent magnetic elements improved the nanoparticle retention in the filters and promoted the mass transfer between the dye and the biocatalyst to enhance wastewater treatment.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Novel Magnetic Polymeric Filters with Laccase-Based Nanoparticles for Improving Congo Red Decolorization in Bioreactors

2022

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“…Specifically, the photo-bioreactors with 20% algal bead volume fractions removed 95% of total Nitrogen and completely reduced total phosphorus in 3 phases of treatment. In another study conducted using immobilised yeast cells (that expressed Laccase from Streptomyces cyaneus), Popović et al completely decoloured Reactive Black 5, Amido Black 10B, Remazol Brilliant Blue, and Evans Blue [163]. Popović et al's findings suggest that dye decolourisation could be carried out using laccasecoated yeast cell walls encapsulated within dopamine-alginate beads (Table 3).…”

Section: Microbial Encapsulationmentioning

confidence: 99%

Alginate-Based Applications in Biotechnology with a Special Mention to Biosensors

Paul¹,

Markus²,

Kristollari³

et al. 2024

Biochemistry

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The exploitation of alginate and its composites as immobilisation support matrices in multiple applications remains a promising field that has the potential to create advanced functional materials from sustainable natural sources. They are non-toxic, allow sol-gel transformation, are biocompatible, have remarkable ion exchange properties, are biodegradable, and are amenable to chemical functionalisation. Alginate and its derived composites have numerous biotechnological and biomedical applications, including biomolecule or cell immobilisation, tissue engineering, drug delivery, wound dressing, and biosensors. Alginate can rapidly crosslink into a stable 3D water-insoluble network called hydrogel with polyvalent cations. Blending alginate with other materials to produce composite materials with improved or novel physicochemical properties remains an ongoing research endeavour. For instance, natural and synthetic polymers or nanoparticles have been incorporated into alginate-yielding composite material with enhanced physical strength, controlled porosity, improved interaction between the alginate support and the biomolecules, and the impartation of other features such as electrical and magnetic responsiveness, among others. Immobilisation strategies are discussed herein, including their innovations and future research perspectives.

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“…Immobilization allows one to enhance the resistance of biocatalysts against inactivation by high temperatures, extreme pHs, organic cosolvents, inhibitors, or shearing forces . In the case of cells, entrapment in calcium alginate is one of the most successful strategies. , …”

Section: Introductionmentioning

confidence: 99%

“…30 In the case of cells, entrapment in calcium alginate is one of the most successful strategies. 31,32 In this work, we have analyzed the reutilization of K. phaff ii cells immobilized in alginate beads for the elimination of Dglucose present in different sugars mixtures, mostly related with prebiotics.…”

Section: ■ Introductionmentioning

confidence: 99%

Reuse of Immobilized Komagataella phaffii Cells for the Elimination of d-Glucose in Syrups of Bioactive Carbohydrates

Cervantes

Fernandez-Polo

Merdzo

et al. 2022

ACS Food Sci. Technol.

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During the synthesis of prebiotic carbohydrates such as fructooligosaccharides (FOS), galactooligosaccharides (GOS), or isomaltooligosaccharides (IMOS), D-glucose is released as a side-product of the transglycosylation process. It is desirable to remove glucose from these sugar mixtures due to its caloric contribution and its effect on caries and diabetes. In this work, we have investigated the use of immobilized Komagataella phaff ii (formerly Pichia pastoris) for elimination of D-glucose and D-fructose in several sugar syrups. K. phaff ii cells were immobilized in calcium alginate beads to facilitate the separation of the yeast cells from the reaction medium and reuse of the biocatalyst. The immobilized yeasts were successfully reutilized for at least 20 cycles (of 195 min) to remove D-glucose (62.3 g/L) and D-fructose (5.5 g/L) in a FOS syrup, without affecting the concentration of oligosaccharides. Excellent selectivity was also found for elimination of D-glucose (57.2 g/L) in IMOS syrups. The methodology is versatile and easy to scale-up, as demonstrated in the removal of D-glucose (97.5 g/L) and D-fructose (142 g/L) for the purification of heteroglucooligosaccharides synthesized by Metschnikowia reukauf ii α-glucosidase. In addition, D-glucose (50 g/L) was selectively removed by K. phaff ii beads in the presence of D-galactose (50 g/L) for at least 20 cycles of 150 min and applied to GOS purification.

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Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization

Cited by 36 publications

References 35 publications

Novel Magnetic Polymeric Filters with Laccase-Based Nanoparticles for Improving Congo Red Decolorization in Bioreactors

Novel Magnetic Polymeric Filters with Laccase-Based Nanoparticles for Improving Congo Red Decolorization in Bioreactors

Alginate-Based Applications in Biotechnology with a Special Mention to Biosensors

Reuse of Immobilized Komagataella phaffii Cells for the Elimination of d-Glucose in Syrups of Bioactive Carbohydrates

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