Immobilization of β-galactosidase from Cicer arietinum (gram chicken bean) and its catalytic actions

Immobilization of b-Galactosidase from Aspergillus oryza on Magnetic Poly(GMA-ST) Beads

“…Activity assays of β β β β β-galactosidase: Activities of free and immobilized β-galactosidase were assayed by the addition of 0.1 mL of free enzyme or 0.0500 g of immobilized β-galactosidase in the citric acid buffer (pH 5.0), using 0.2 mL of ONPG (1.5 mg/mL) as the substrate 5,6 . After 15 min of incubation in a shaking water bath at 55 ºC, the reaction was stopped by adding 2 mL of Na2CO3 solution (1 M).…”

Preparation of Nano-Sized Mesoporous Silica and Its Application in Immobilization of β-galactosidase from Aspergillus Oryzae

“…Then, with 2.0 mL glutaraldehyde (25 %) and 8.0 mL diethanolamine mixed together and stirred for 30 minutes at 20 ºC, 1.0000 g amino silica obtained above was added (Tu et al, 1999), the precipitate was obtained with constant stirring for 4 h at 30 ºC, after being washed completely with distilled water, it was put into HCl solution (0.1 mol/L). The activated mesoporous silica was obtained after 30 minutes with stirring.…”

Preparation of Micron Sized Mesoporous Silica and its use to Immobilize β-galactosidase from Aspergillus Oryzae

“…Then, amino mesoporous silica was obtained by filtration, washed with toluene and acetone thoroughly, and dried 12 h at 80 ºC vacuum, then put in a desiccator to be used in the next step. Finally, with 2.0 mL 25 % glutaraldehyde and 8.0 mL diethanolamine being mixed together and stirred 30 min at 20 ºC, 1.0000 g amino mesoporous silica obtained above was added [11], the precipitate was obtained with constant stirring for 4 h at 30 ºC, after being washed completely with distilled water, it was put into 0.1 mol/L HCl solution. The aldehyde mesoporous silica was obtained after 30 min with stirring.…”