Immobilized 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole as solid phase luminophore in peroxyoxalate chemiluminescence

Pontén, Einar; Appelblad, Patrik; Stigbrand, Malin; Irgum, Knut; Nakashima, Kenichiro

doi:10.1007/s0021663560084

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…[27][28][29] Applying a similar approach, Pontén et al compared six amino-based luminophores employing porous and non-porous methacrylate beads. [30][31][32] These authors concluded that porous materials were much more sensitive due to a higher surface area and degree of functionalization. To the best of our knowledge, this study from the mid-nineties is the only reported MS solid phase luminophore.…”

Section: Introductionmentioning

confidence: 99%

A Photochemical Ligation System Enabling Solid‐Phase Chemiluminescence Read‐Out

Delafresnaye

Schmitt

Barner

et al. 2019

Chemistry A European J

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The peroxyoxalate chemiluminescence (PO-CL) reaction is among the most powerful and versatile techniques for the detection of hydrogen peroxide (H 2 O 2 ) and has been employed in various biological and chemical applications over the past 50 years. However, its twocomponent nature (peroxyoxalate and fluorophore) limits its use. This contribution introduces an innovative and versatile photochemical platform technology for the synthesis of inherently fluorescent PO probes by exploiting the nitrile imine-mediated tetrazole-ene cycloaddition (NITEC) reaction. In the presence of hydrogen peroxide, the p "2-in-1" emits either yellow or blue light -p g z ' ( z) visible to the naked eye. Even in the absence of base, the emitted light remains visible and H 2 O 2 could be detected in the nanomolar range. Critically, the Tz-PO can be readily incorporated into polymeric materials. As a first application of this promising material, a tailor-made Tz-PO is

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Section: Introductionmentioning

confidence: 99%

A Photochemical Ligation System Enabling Solid‐Phase Chemiluminescence Read‐Out

Delafresnaye

Schmitt

Barner

et al. 2019

Chemistry A European J

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“…More than 5000 samples were injected during this period. Apart from the ease of preparation of the ISPS reactors and the fact that no frits are needed, which should be of considerable merit in many other applications, we did not experience any problems with “fines” increasing the back-pressure during long-term use. It may be concluded that this polymer composition is sufficiently rugged for practical use.…”

Section: Resultsmentioning

confidence: 96%

Solid Phase Chemiluminescence Detection Reactors Based on in Situ Polymerized Methacrylate Materials

et al. 1996

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In situ photopolymerized macroporous poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) materials, which were prepared in a full factorial experimental synthesis design, were investigated as supports in solid phase chemiluminescence detection reactors. The reactors based on in situ polymerized supports showed higher light generation efficiency than packed bed reactors when evaluated in a flow system based on 1,1′-oxalyldiimidazolyl peroxyoxalate chemiluminescence detection of hydrogen peroxide, with 3-aminofluoranthene (3-AFA) as the immobilized light emitter. The results were correlated with the physical characteristics of the materials, and the efficiency was found to correlate with the amount of accessible reactive groups. A lower functionalization density was found to increase the peak area sensitivity for hydrogen peroxide in the flow system. This is explained by inner filtering. The peak height sensitivities were less influenced, indicating that the total system efficiency was limited by homogeneous reaction kinetics. The introduction of a spacer to mimic "pseudosolution" conditions of the bound 3-AFA moiety was found to decrease the light generation ability.

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“…A great many procedures have been proposed to determine hydrogen peroxide. Examples include electrochemical methods (3,4), spectrophotometric methods (5,6), fluorescence techniques (7,8), and chemiluminescence methods (9,10). Nowadays, the most widely used methods for the determination of hydrogen peroxide in clinical chemistry are the spectrophotometric procedures by reaction with a chromogenic hydrogen donor substrate in the presence of peroxidase (2).…”

Section: Introductionmentioning

confidence: 99%