Immobilized galactose oxidase in alginate gel (GO-Bead): a versatile and efficient biocatalyst for the regioselective synthesis of 1,4-disubstitued-1,2,3-triazoles: click reaction

Gourdani, Fateme Akbari; Ghadam, Parinaz; Heravi, Majid M.; Malmir, Masoumeh

doi:10.1007/s13738-020-02128-1

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…It can catalyze chemical reactions under light irradiation, and has the advantages of high efficiency, controllability, and repeatability. For example, a robust synthetic oxidoreductase was successfully designed to mimic the catalytic performance of galactose oxidase, and related biocatalysts, under irradiation [54]. Moreover, a CdS-Pt nanoparticle system prepared via the peptide-mediated synthesis method, behaved as a robust inorganic mimic in the reduction of nitrate to nitrite, with an activity level that outperformed the nitrate reductase enzyme > 23-fold [55].…”

Section: Of 17mentioning

confidence: 99%

Plant Photocatalysts: Photoinduced Oxidation and Reduction Abilities of Plant Leaf Ashes under Solar Light

Liu

et al. 2023

Nanomaterials

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Plant leaf ashes were obtained via the high temperature calcination of the leaves of various plants, such as sugarcane, couchgrass, bracteata, garlic sprout, and the yellowish leek. Although the photosynthesis systems in plant leaves cannot exist after calcination, minerals in these ashes were found to exhibit photochemical activities. The samples showed solar light photocatalytic oxidation activities sufficient to degrade methylene blue dye. They were also shown to possess intrinsic dehydrogenase-like activities in reducing the colorless electron acceptor 2,3,5-triphenyltetrazolium chloride to a red formazan precipitate under solar light irradiation. The possible reasons behind these two unreported phenomena were also investigated. These ashes were characterized using a combination of physicochemical techniques. Moreover, our findings exemplify how the soluble and insoluble minerals in plant leaf ashes can be synergistically designed to yield next-generation photocatalysts. It may also lead to advances in artificial photosynthesis and photocatalytic dehydrogenase.

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Section: Of 17mentioning

confidence: 99%

Plant Photocatalysts: Photoinduced Oxidation and Reduction Abilities of Plant Leaf Ashes under Solar Light

Liu

et al. 2023

Nanomaterials

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“…Furthermore, enzymatic reactions are performed under mild conditions and result in limited by-products. [3][4][5][6] However, enzymes used on a large scale face some challenges, including difficulty of enzyme recycling, high cost, and low operational stability. To overcome these problems, enzyme immobilization is an effective method.…”

Section: Introductionmentioning

confidence: 99%

“…To overcome these problems, enzyme immobilization is an effective method. 3,[7][8][9][10][11] Enzyme immobilization procedures can be classified into two groups: carrier-assisted and carrier-free immobilization methods. The former involves covalent binding, adsorption, entrapment, and encapsulation.…”

Section: Introductionmentioning

confidence: 99%

In silico approaches to investigate enzyme immobilization: a comprehensive systematic review

Barati,

Hosseini,

Vafaee

et al. 2024

Phys. Chem. Chem. Phys.

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“…For this purpose, GalOx has been immobilized on cellulose acetate membranes, [15] natural materials such as eggshell, [16] collagen, [17] and laponite clay, [18] ZnO nanorods, [19] or entrapped in a poly (glycidyl methacrylate‐co‐vinylferrocene) (poly (GMA‐co‐VFc)) film [20] . More recently, GalOx has been immobilized for its use in synthetic biotransformations, e. g. in alginate beads to synthesize 1,2,3‐triazoles via click chemistry [21] . To achieve increased storage stability, GalOx was immobilized on hydrazide‐modified cellulose microporous beads and microspheres made from hydroxyethyl methacrylate and ethylene dimethacrylate (poly(HEMA‐co‐EDMA) [22] .…”

Section: Introductionmentioning

confidence: 99%

Selective Oxidation of 5‐Hydroxymethylfurfural to 2,5‐Diformylfuran in Biphasic Media using Immobilized Galactose Oxidase: Proof of Concept and Limitations

Milić,

Byström,

Domínguez de María

et al. 2024

ChemCatChem

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The oxidation of 5‐hydroxymethylfurfural (HMF) to 2,5‐diformylfuran (DFF) is a key reaction in valorizing biomass. DFF is hardly soluble in water, while HMF is often obtained from biorefineries in crude wet organic fractions. Thus, the reaction is challenging for both biocatalysis performed in aqueous media, and chemocatalysis where the presence of water often results in catalyst poisoning. Galactose oxidase (GalOx) can selectively oxidize HMF to DFF and displays promising activity in aqueous‑organic media. In this study, GalOx was immobilized on ten carriers, assessing the immobilization yield, activity, and stability. Covalently immobilized GalOx catalyzed the oxidation of HMF to DFF in neat and water‐saturated EtOAc, and in biphasic systems of various water contents. At 50% v/v H2O, the reaction was conducted at semi‐preparative scale (50 mL) with no adverse effect on DFF yield. Some limitations arise, such as enzyme deactivation, and adsorption of DFF to the support, particularly in the aqueous fraction. Future options to upgrade the route may include designed stable enzymes under the presence of HMF/DFF, and the setup of microaqueous systems where DFF adsorption is minimized. The use of wet EtOAc media would be a promising approach in future biorefineries employing inexpensive crude wet organic fractions.

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Immobilized galactose oxidase in alginate gel (GO-Bead): a versatile and efficient biocatalyst for the regioselective synthesis of 1,4-disubstitued-1,2,3-triazoles: click reaction

Cited by 5 publications

References 36 publications

Plant Photocatalysts: Photoinduced Oxidation and Reduction Abilities of Plant Leaf Ashes under Solar Light

Plant Photocatalysts: Photoinduced Oxidation and Reduction Abilities of Plant Leaf Ashes under Solar Light

In silico approaches to investigate enzyme immobilization: a comprehensive systematic review

Selective Oxidation of 5‐Hydroxymethylfurfural to 2,5‐Diformylfuran in Biphasic Media using Immobilized Galactose Oxidase: Proof of Concept and Limitations

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