2016
DOI: 10.1016/j.chroma.2016.03.044
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Immobilized metal affinity chromatography on collapsed Langmuir-Blodgett iron(III) stearate films and iron(III) oxide nanoparticles for bottom-up phosphoproteomics

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Cited by 22 publications
(15 citation statements)
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“…These bottle necks of GC-MS-and LC-MS-based approaches for FA analysis can be efficiently overcome by implementation of matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS) in combination with Langmuir-Blodgett technology [21]. Recently, we have shown that Langmuir-Blodgett films can be not only efficiently implemented in selective enrichment of phosphopeptides [22], protein adducts of organophosphorous compounds [23] and chlorinated insecticides [24], but also proved to be an efficient tool for high-throughput and sensitive fingerprinting of free fatty acids as their barium monocarboxylates in positive ion mode [25]. Therefore, here we extend this approach to analysis of FFA composition of rhizobial bacteria and propose a comprehensive protocol for fingerprinting of FFAs in cultured cells by MALDI-TOF-MS.…”
Section: Figure 1 Structural Class Of Bacterial Fatty Acidsmentioning
confidence: 99%
“…These bottle necks of GC-MS-and LC-MS-based approaches for FA analysis can be efficiently overcome by implementation of matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS) in combination with Langmuir-Blodgett technology [21]. Recently, we have shown that Langmuir-Blodgett films can be not only efficiently implemented in selective enrichment of phosphopeptides [22], protein adducts of organophosphorous compounds [23] and chlorinated insecticides [24], but also proved to be an efficient tool for high-throughput and sensitive fingerprinting of free fatty acids as their barium monocarboxylates in positive ion mode [25]. Therefore, here we extend this approach to analysis of FFA composition of rhizobial bacteria and propose a comprehensive protocol for fingerprinting of FFAs in cultured cells by MALDI-TOF-MS.…”
Section: Figure 1 Structural Class Of Bacterial Fatty Acidsmentioning
confidence: 99%
“…Different strategies have recently been proposed, ranging from the derivation of peptides between successive separation dimensions [102], to the combination of different LC principles using multidimensional nanoLC, such as ion exchange (IE) [103,104], Hydrophilic interaction chromatography (HILIC) [105], or 2D high pH reversed-phase/low pH reversed-phase chromatography (hi RP-lo RP) [106,107], or 3D RPLC-MS [108], or using electrophoresis as the capillary zone electrophoresis (CZE)-MS coupling [109] for greater complementarity [110]. A study on more specific stationary phases was proposed for applications without a priori [111,112] or for targeted applications such as phosphoproteomics [113], glycoproteomics [114], detection of angiotensin [115], or detection of amyloid peptide [116]. For example, 2D nanoLC coupling has increased the sensitivity by two orders of magnitude in MS mode to reach a sub-attomolar detection limit [117].…”
Section: Separative Techniquesmentioning
confidence: 99%
“…Most often, 7 -8 mol/L urea and 2 mol/L thiourea are used as chaotropic agents (i.e. compounds, disrupting the hydrogen bonding network of water) [71], whereas 3-(3-cholamidopropyl)dimethylammonio)-1propanesulfonate (CHAPS) [72] and Triton X-100 are typically used as detergents (i.e. substances, disrupting intra-and inter-molecule non-polar interactions) [73].…”
Section: Gel-based Bottom-up Proteomicsmentioning
confidence: 99%