Immobilized random peptide mixtures exhibit broad antimicrobial activity with high selectivity

Cheriker, Hadar; Bauer, Tal Stern; Oren, Yuval; Nir, Shlomo; Hayouka, Zvi

doi:10.1039/d0cc04759g

Cited by 5 publications

(7 citation statements)

References 41 publications

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“…On the other hand, the system described in [26] appears to be much more efficient in the removal of the toxin, due to the development of bacterial biofilms that degrade microcystins. Recently, we developed a model that simulates filtration in which a biological degradation component was added to the sorption/desorption processes [27]. For an efficient and reliable procedure of the removal of cyanotoxins, it is suggested to combine two elements in series, a filter with activated carbon followed by the micelle-clay one.…”

Section: Simulation and Prediction Of Toxin Filtration By The Odtma-bmentioning

confidence: 99%

Removal of Cyanotoxins–Microcystins from Water by Filtration through Granulated Composites of Bentonite with Micelles of the Cation Octadecyltrimethyl Ammonium (ODTMA)

et al. 2021

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Cyanobacteria and their toxins present potential hazards to consumers of water from lakes, reservoirs and rivers; thus, their removal via water treatment is essential. Previously, we demonstrated that nanocomposites of octadecyltrimethyl ammonium (ODTMA) complexed with clay could efficiently remove cyanobacteria and their toxins from laboratory cultures and lake water. In this study, we determined the capacity of ODTMA nanocomposites to remove cyanotoxins, namely microcystins (MCs), from water to below 1 µg/L via filtration. This capacity was 1500 mg MC-LR per Kg of nanocomposite. Similar capacities were estimated for the removal of other MC congeners (MC-WR, MC-3aspWR and MC-YR), whereas substantially lower capacities were recorded for more positively charged MC congeners, such as MC-RR and MC-3aspRR. Filtration results were simulated with a filtration model, which considers convection and adsorption/desorption of one to several toxins. Model calculations for the removal of MC-LR, under a variety of situations, fitted well with all the experimentally measured values and also estimated the co-removal of several MC congeners. In agreement with model predictions, results demonstrated that in the presence of MC-WR, the emerging concentrations of MC-RR congeners eventually exceed their solution values. In conclusion, granulated nanocomposites of ODTMA–bentonite can be applied for the removal of microcystins from drinking water.

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Section: Simulation and Prediction Of Toxin Filtration By The Odtma-bmentioning

confidence: 99%

Removal of Cyanotoxins–Microcystins from Water by Filtration through Granulated Composites of Bentonite with Micelles of the Cation Octadecyltrimethyl Ammonium (ODTMA)

et al. 2021

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“…Dathe and co-workers have illustrated the importance of the spacer length for optimal antimicrobial activity for surface-tethered AMPs, , and Costa et al further reported the difference between N and C-terminal surface attachment on the antimicrobial effect of the AMP Dhvar5 . The study by Costa et al also highlighted how the adsorption of an active AMP in a non-optimized manner could induce bacterial surface adhesion illustrating the importance of control over the surface coupling events. , In contrast, other studies report bioactive surfaces based on random covalent attachment of AMPs . Recently, the Chain201D peptide derived from crowberry epiphytes was shown by Monteiro et al to display powerful surface killing properties when immobilized on gold surfaces despite being attached in a nonspecific manner via the peptide lysines and N-terminus …”

Section: Resultsmentioning

confidence: 99%

“…42,60 In contrast, other studies report bioactive surfaces based on random covalent attachment of AMPs. 70 Recently, the Chain201D peptide derived from crowberry epiphytes was shown by Monteiro et al to display powerful surface killing properties when immobilized on gold surfaces despite being attached in a nonspecific manner via the peptide lysines and N-terminus. 40 In our current work, we employ a peptide scaffold that interferes with the ability of bacteria to adhere to surfaces via EPS.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Efficient Prevention of Marine Biofilm Formation Employing a Surface-Grafted Repellent Marine Peptide

Herzberg

Berglin

Eliahu

et al. 2021

ACS Appl. Bio Mater.

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Creation of surfaces resistant to the formation of microbial biofilms via biomimicry has been heralded as a promising strategy to protect a range of different materials ranging from boat hulls to medical devices and surgical instruments. In our current study, we describe the successful transfer of a highly effective natural marine biofilm inhibitor to the 2D surface format. A series of cyclic peptides inspired by the natural equinatoxin II protein produced by Beadlet anemone (Actinia equine) have been evaluated for their ability to inhibit the formation of a mixed marine microbial consortium on polyamide reverse osmosis membranes. In solution, the peptides are shown to effectively inhibit settlement and biofilm formation in a nontoxic manner down to 1 nM concentrations. In addition, our study also illustrates how the peptides can be applied to disperse already established biofilms. Attachment of a hydrophobic palmitic acid tail generates a peptide suited for strong noncovalent surface interactions and allows the generation of stable noncovalent coatings. These adsorbed peptides remain attached to the surface at significant shear stress and also remain active, effectively preventing the biofilm formation over 24 h. Finally, the covalent attachment of the peptides to an acrylate surface was also evaluated and the prepared coatings display a remarkable ability to prevent surface colonization at surface loadings of 55 ng/cm2 over 48 h. The ability to retain the nontoxic antibiofilm activity, documented in solution, in the covalent 2D-format is unprecedented, and this natural peptide motif displays high potential in several material application areas.

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“…The authors concluded that an active biofilm formed at the surface of the GAC. In this context we add that a successful modeling of the kinetics of filtration followed by degradation was developed has been recently described for removal of bacteria by dipeptides immobilized by beads [123].…”

Section: Bacteriamentioning

confidence: 99%

Applications of Chemically Modified Clay Minerals and Clays to Water Purification and Slow Release Formulations of Herbicides

et al. 2020

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This review deals with modification of montmorillonite and other clay-minerals and clays by interacting them with organic cations, for producing slow release formulations of herbicides, and efficient removal of pollutants from water by filtration. Elaboration is on incorporating initially the organic cations in micelles and liposomes, then producing complexes denoted micelle- or liposome-clay nano-particles. The material characteristics (XRD, Freeze-fracture electron microscopy, adsorption) of the micelle– or liposome–clay complexes are different from those of a complex of the same composition (organo-clay), which is formed by interaction of monomers of the surfactant with the clay-mineral, or clay. The resulting complexes have a large surface area per weight; they include large hydrophobic parts and (in many cases) have excess of a positive charge. The organo-clays formed by preadsorbing organic cations with long alkyl chains were also addressed for adsorption and slow release of herbicides. Another examined approach includes “adsorptive” clays modified by small quaternary cations, in which the adsorbed organic cation may open the clay layers, and consequently yield a high exposure of the siloxane surface for adsorption of organic compounds. Small scale and field experiments demonstrated that slow release formulations of herbicides prepared by the new complexes enabled reduced contamination of ground water due to leaching, and exhibited enhanced herbicidal activity. Pollutants removed efficiently from water by the new complexes include (i) hydrophobic and anionic organic molecules, such as herbicides, dissolved organic matter; pharmaceuticals, such as antibiotics and non-steroidal drugs; (ii) inorganic anions, e.g., perchlorate and (iii) microorganisms, such as bacteria, including cyanobacteria (and their toxins). Model calculations of adsorption and kinetics of filtration, and estimation of capacities accompany the survey of results and their discussion.

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Immobilized random peptide mixtures exhibit broad antimicrobial activity with high selectivity

Abstract: In the current study, we showed that randomly-sequenced peptide mixtures (RPMs) that were immobilized on beads exhibit high and broad bactericidal activity against various pathogenic bacteria while possessing minimal hemolytic activity.

Cited by 5 publications

References 41 publications

Removal of Cyanotoxins–Microcystins from Water by Filtration through Granulated Composites of Bentonite with Micelles of the Cation Octadecyltrimethyl Ammonium (ODTMA)

Removal of Cyanotoxins–Microcystins from Water by Filtration through Granulated Composites of Bentonite with Micelles of the Cation Octadecyltrimethyl Ammonium (ODTMA)

Efficient Prevention of Marine Biofilm Formation Employing a Surface-Grafted Repellent Marine Peptide

Applications of Chemically Modified Clay Minerals and Clays to Water Purification and Slow Release Formulations of Herbicides

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