1993
DOI: 10.1016/0014-5793(93)80854-n
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Immobilizing DNA on gold via thiol modification for atomic force microscopy imaging in buffer solutions

Abstract: Thiols, dialkylsulfides, and dialkyldisuliides are known to be chemisorbed with high afiity on gold. We have prepared DNAs of specific length and sequence carrying thiol groups at each end. For this purpose, primers with an HS-(CH,),-arm at the Y-end were used to amplify segments of plasmid DNA via the polymerase chain reaction. These thiolated DNAs bind strongly to the large, ultraflat Au surfaces which we have recently described [Hegner, M. et al. (1993) Surface Sci. 291, 39-461, and can be imaged by AFM in … Show more

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Cited by 108 publications
(79 citation statements)
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“…was not sufficient for achieving high resolution in aqueous solution [14][15][16][17]. Even when covalently linked to the substrate [18,19]. the resolution was still insufficient.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…was not sufficient for achieving high resolution in aqueous solution [14][15][16][17]. Even when covalently linked to the substrate [18,19]. the resolution was still insufficient.…”
Section: Resultsmentioning
confidence: 99%
“…see [I]). as is the capability to observe DNA-protein interactions in solution [13][14][15][16][17][18][19][20]. Although many arguments have been proposed to account for this unexpected low resolution [9.10].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…To this end, specimens may be covalently linked to a chemically modified support (Karrasch et al, 1993;Hegner et al, 1993Hegner et al, , 1996Wagner et al, 1994Wagner et al, , 1995. Most of the time, however, physisorption is sufficient.…”
Section: Introductionmentioning
confidence: 99%
“…However, only a few studies have generated pertinent structural information regarding the binding and conformation of oligonucleosides and their building blocks on gold surfaces. 10,11 Hereby self-assembled monolayers of DNA strands on gold surface have been studied with SERS and data were compared with SERS spectra of oligonucleosides in aqueous gold nanoparticles solution. 12 Based on this study, the coordination structures of the DNA strands on gold surfaces are proposed.…”
mentioning
confidence: 99%