Immortalized cells as experimental models to study cancer

Boehm, Jesse S.; Hahn, William C.

doi:10.1007/s10616-004-5125-1

Cited by 17 publications

(16 citation statements)

References 100 publications

(144 reference statements)

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Section: Cell Lines As Model Systemsmentioning

confidence: 99%

“…4 Moreover, the genetic changes required to establish continuous cell lines from normal cells recapitulate many of the genetic changes occurring in cancer. 5,6 These genetic changes are required to overcome replicative senescence, in which normal cells continue to be metabolically active but are restricted from further division. 1 Cells able to overcome senescence continue proliferating until their telomeres become so short that the chromosomes undergo fusion-breakage-bridge cycles and the ensuing genomic instability results in culture crisis.…”

Section: Cell Lines As Model Systemsmentioning

confidence: 99%

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Check your cultures! A list of cross‐contaminated or misidentified cell lines

Capes‐Davis

Theodosopoulos

Atkin³

et al. 2010

Intl Journal of Cancer

436

378

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Continuous cell lines consist of cultured cells derived from a specific donor and tissue of origin that have acquired the ability to proliferate indefinitely. These cell lines are well‐recognized models for the study of health and disease, particularly for cancer. However, there are cautions to be aware of when using continuous cell lines, including the possibility of contamination, in which a foreign cell line or microorganism is introduced without the handler's knowledge. Cross‐contamination, in which the contaminant is another cell line, was first recognized in the 1950s but, disturbingly, remains a serious issue today. Many cell lines become cross‐contaminated early, so that subsequent experimental work has been performed only on the contaminant, masquerading under a different name. What can be done in response—how can a researcher know if their own cell lines are cross‐contaminated? Two practical responses are suggested here. First, it is important to check the literature, looking for previous work on cross‐contamination. Some reports may be difficult to find and to make these more accessible, we have compiled a list of known cross‐contaminated cell lines. The list currently contains 360 cell lines, drawn from 68 references. Most contaminants arise within the same species, with HeLa still the most frequently encountered (29%, 106/360) among human cell lines, but interspecies contaminants account for a small but substantial minority of cases (9%, 33/360). Second, even if there are no previous publications on cross‐contamination for that cell line, it is essential to check the sample itself by performing authentication testing.

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Section: Cell Lines As Model Systemsmentioning

confidence: 99%

Section: Cell Lines As Model Systemsmentioning

confidence: 99%

Check your cultures! A list of cross‐contaminated or misidentified cell lines

Capes‐Davis

Theodosopoulos

Atkin³

et al. 2010

Intl Journal of Cancer

436

378

View full text Add to dashboard Cite

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“…The glycoprotein interferon has been used for the treatment of a variety of conditions including hepatitis B and C [17], cervical intraepithelial neoplasia [18], cutaneous squamous neoplasia [19] and as off-label treatment of OSSN [20][21][22][23]. Interferon is an immuno modulator that consists of 165 amino acid residues, with arginine in position 23 [24].…”

Section: Ifn-α 2bmentioning

confidence: 99%

“…All lesions showed reduction in size, with 72% of cases showing complete resolution. Topical IFN-α 2b is normally given off-label four times a day for 3-4 months [20,22,23] and needs compounding and refrigeration. With intralesional interferon, there is no need for compounding, it is widely available, compliance is ensured and a more rapid resolution is observed with a median resolution time between 1.4 [30] and 1.1 months [21].…”

Section: Ifn-α 2bmentioning

confidence: 99%

Modern management of ocular surface squamous neoplasia

Tsatsos

Karp

2013

Expert Review of Ophthalmology

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Ocular surface squamous neoplasia (OSSN) consists of a wide range of conjuctival and corneal lesions ranging from dysplastic lesions to invasive squamous cell carcinoma. A number of risk factors, including genetic predisposition, immunosuppression, HPV infection, ultraviolet exposure and smoking, have been associated with the appearance of OSSN. The authors discuss the recent advances in the management of OSSN, and describe surgical techniques and medical approaches to treat this disease. The authors believe that ultra-high-resolution optical coherence tomography will provide invaluable help in the diagnosis of surface lesions and their recurrences in the near future. Modern management of ocular surface squamous neoplasia Expert Rev. Ophthalmol. 8(3), 287-295 (2013) Keywords: IFN-α 2b • mitomycin C • no-touch surgical excision • ocular surface squamous neoplasia • ultra-high-resolution optical coherence tomography For reprint orders, please contact reprints@expert-reviews.com Expert Review of Ophthalmology Downloaded from informahealthcare.com by Nyu Medical Center on 07/26/15 For personal use only. •• A study of almost 400 lesions revealing increased recurrences with positive margins and decreased recurrences when cryotherapy was utilitized. This study also showed that the use of postoperative interferon drops decreased the rates of recurrences when used with positive margins. 76 Erie JC, Campbell RJ, Liesegang TJ. Conjunctival and corneal intraepithelial and invasive neoplasia. Ophthalmology 93(2), 176-183 (1986). 77 Lee GA, Hirst LW. Retrospective study of ocular surface squamous neoplasia.

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“…The lack of mTERT-mediated immortalization in well-differentiated mouse cells might be due to the intrinsically higher level of TERT expression in mouse compared with human cells, the delivery method of the TERT gene, or cell line variability (7,19,29).…”

Section: Epithelial; Polycysticmentioning

confidence: 99%

Telomerase immortalization of principal cells from mouse collecting duct

Steele¹,

Kolb

et al. 2010

American Journal of Physiology-Renal Physiology

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Recently, the use of overexpression of telomerase reverse transcriptase (TERT) has led to the generation of immortalized human cell lines. However, this cell immortalization approach has not been reported in well-differentiated mouse cells, such as renal epithelial cells. We sought to establish and then characterize a mouse collecting duct cell line, using ectopic expression of mTERT. Isolated primary cortical collecting duct (CCD) cell lines were transduced with mouse (m)TERT, using a lentiviral vector. mTERT-negative cells did not survive blasticidin selection, whereas mTERT-immortalized cells proliferated in selection media for over 40 subpassages. mTERT messenger RNA and telomerase activity was elevated in these cells, compared with an SV40-immortalized cell line. Flow cytometry with Dolichos biflorus agglutinin was used to select the CCD principal cells, and we designated this cell line mTERT-CCD. Cells were well differentiated and exhibited morphological characteristics typically found in renal epithelial cells, such as tight junction formation, microvilli, and primary cilia. Further characterization using standard immunofluorescence revealed abundant expression of aquaporin-2 and the vasopressin type 2 receptor. mTERT-CCD cells exhibited cAMP-stimulated/benzamil-inhibited whole cell currents. Whole cell patch-clamp currents were also enhanced after a 6-day treatment with aldosterone. In conclusion, we have successfully used mTERT to immortalize mouse collecting duct cells that retain the basic in vivo phenotypic characteristics of collecting duct cells. This technique should be valuable in generating cell lines from genetically engineered mouse models.

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Immortalized cells as experimental models to study cancer

Cited by 17 publications

References 100 publications

Check your cultures! A list of cross‐contaminated or misidentified cell lines

Check your cultures! A list of cross‐contaminated or misidentified cell lines

Modern management of ocular surface squamous neoplasia

Telomerase immortalization of principal cells from mouse collecting duct

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