Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology

Lathuilière, Aurélien; Vernet, Remi; Charrier, Emily; Urwyler, Muriel; Rohr, Olivier; Belkouch, Marie-Claude; Saingier, Valentin; Bouvarel, Thomas; Guillarme, Davy; Engel, Adrien; Salmon, Patrick; Laumonier, Thomas; Grogg, Julien; Mach, Nicolas

doi:10.1016/j.omtm.2022.07.017

Cited by 6 publications

(5 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another experiment, we maintained Myo-P devices containing human myoblast cells secreting huGM-CSF (MVX-2/CCOS1901) in culture for 33 months, and the secretion of huGM-CSF in culture supernatant ranged from 613 to 1173 ng/24 h/capsule over time ( Figure 4 A). We previously showed that Myo-P encapsulated huGM-CSF and anti-CD20 antibody-secreting cells implanted in the subcutaneous tissue of Rag2/IL2rg−/− mice produced both proteins to a measurable extent in the serum over 43 weeks 33 The latter model does not develop functional B, T, and NK cells, which results in a deficient adaptive immune response. It was selected to mitigate the effect of xenogeneic engineered transplanted cells on the immune system and avoid a neutralizing anti-drug response that may have altered the blood level of delivered recombinant protein.…”

Section: Resultsmentioning

confidence: 99%

“…MVX-2 cells were selected because they are a nontumorigenic, human myoblast cell line which displays contact inhibition, are amenable to genetic modification, and have been characterized for clinical use. 33 The huGM-CSF, as well as a selected panel of proinflammatory cytokine concentrations, was measured in adjacent tissue at days 1, 3, 5, and 7 to characterize both the pharmacokinetic and the pharmacodynamic of the intervention. A 25-ng bolus injection of recombinant huGM-CSF was used as comparator.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins

Grogg¹,

Vernet²,

Charrier³

et al. 2023

iScience

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins

Grogg¹,

Vernet²,

Charrier³

et al. 2023

iScience

Self Cite

View full text Add to dashboard Cite

“…Encapsulated cell technology is also a useful approach for the continuous and local delivery of genetically modified therapeutic proteins. However, the clinical development of encapsulated cell technology to deliver therapeutic proteins from macro-capsules is still limited due to the lack of a compatible allogeneic cell line for therapeutic purposes in humans [ 33 ].…”

Section: The Application Of Immortalized Cell Linesmentioning

confidence: 99%

Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and Challenges

Chalak,

Hesaraki,

Mirbahari

et al. 2024

Life

View full text Add to dashboard Cite

Cells are very important to researchers due to their use in various biological studies in in vitro and in vivo settings. This importance stems from the short lifespan of most cells under laboratory conditions, which can pose significant challenges, such as the difficulties associated with extraction from the source tissue, ethical concerns about separating cells from human or animal models, limited cell passage ability, and variation in results due to differences in the source of the obtained cells, among other issues. In general, cells in laboratory conditions can divide into a limited number, known as the Hayflick limit, due to telomere erosion at the end of each cellular cycle. Given this problem, researchers require cell lines that do not enter the senescence phase after a limited number of divisions. This can allow for more stable studies over time, prevent the laborious work associated with cell separation and repeated cultivation, and save time and money in research projects. The aim of this review is to summarize the function and effect of immortalization techniques, various methods, their advantages and disadvantages, and ultimately the application of immortalization and cell line production in various research fields.

show abstract

“…These cells also cause little concern in terms of oncogenic potential since they are planned to be used only in capsules, which prevents the cells from encountering the internal body environment, and it is possible to remove the capsules at any time. The authors state that they plan to conduct a stage I clinical trial with a GM-CSF-producing variant of these cells for tumor therapy [86]. The possible areas of application of immortalized cells as substance deliverers are summarized in Thus, immortalized cell lines are being actively studied and compete with primary cells and other methods in the field of targeted therapy of oncological diseases and the delivery of therapeutic substances.…”

Section: Immortalized Cells As Deliverersmentioning

confidence: 99%

Practical Use of Immortalized Cells in Medicine: Current Advances and Future Perspectives

Voloshin

Tyurin‐Kuzmin

Karagyaur

et al. 2023

IJMS

View full text Add to dashboard Cite

In modern science, immortalized cells are not only a convenient tool in fundamental research, but they are also increasingly used in practical medicine. This happens due to their advantages compared to the primary cells, such as the possibility to produce larger amounts of cells and to use them for longer periods of time, the convenience of genetic modification, the absence of donor-to-donor variability when comparing the results of different experiments, etc. On the other hand, immortalization comes with drawbacks: possibilities of malignant transformation and/or major phenotype change due to genetic modification itself or upon long-term cultivation appear. At first glance, such issues are huge hurdles in the way of immortalized cells translation into medicine. However, there are certain ways to overcome such barriers that we describe in this review. We determined four major areas of usage of immortalized cells for practical medicinal purposes, and each has its own means to negate the drawbacks associated with immortalization. Moreover, here we describe specific fields of application of immortalized cells in which these problems are of much lesser concern, for example, in some cases where the possibility of malignant growth is not there at all. In general, we can conclude that immortalized cells have their niches in certain areas of practical medicine where they can successfully compete with other therapeutic approaches, and more preclinical and clinical trials with them should be expected.

show abstract

Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology

Cited by 6 publications

References 68 publications

Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins

Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins

Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and Challenges

Practical Use of Immortalized Cells in Medicine: Current Advances and Future Perspectives

Contact Info

Product

Resources

About