Immumochromatographic assay for determination of hexoestrol residues

Huo, Tieming; Peng, Chifang; Xu, Chuanlai; Liu, Liqiang

doi:10.1007/s00217-006-0477-8

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…Owing to their high specificity and sensitivity, these strip tests have found a wide utility in the detection of narcotics, toxins, highly dangerous infections, and urogenital diseases. [202][203][204][205][206][207][208] Methods have been developed for the diagnosis of tuberculosis, 209 helicobacteriosis, 210 staphylococcal infection, 211 hepatitis B, 212 prostatitis, 213 and early pregnancy, 214 for DNA hybridization; 215 for the detection of pesticides, 216 aflatoxin, 217 hexoestrol, 218 and cephalexin 219 in environmental constituents; and for other purposes.…”

Section: Gnps In Diagnostics 21 Visualization and Bioimaging Methodsmentioning

confidence: 99%

Gold nanoparticles in biomedical applications: recent advances and perspectives

2012

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Gold nanoparticles (GNPs) with controlled geometrical, optical, and surface chemical properties are the subject of intensive studies and applications in biology and medicine. To date, the ever increasing diversity of published examples has included genomics and biosensorics, immunoassays and clinical chemistry, photothermolysis of cancer cells and tumors, targeted delivery of drugs and antigens, and optical bioimaging of cells and tissues with state-of-the-art nanophotonic detection systems. This critical review is focused on the application of GNP conjugates to biomedical diagnostics and analytics, photothermal and photodynamic therapies, and delivery of target molecules. Distinct from other published reviews, we present a summary of the immunological properties of GNPs. For each of the above topics, the basic principles, recent advances, and current challenges are discussed (508 references).

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Section: Gnps In Diagnostics 21 Visualization and Bioimaging Methodsmentioning

confidence: 99%

Gold nanoparticles in biomedical applications: recent advances and perspectives

2012

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“…The sample, added onto the sample pad, migrates through the membrane and is captured by the antibodies immobilized within the nitrocellulose membrane to produce a visible and putatively measurable colorimetric signal. There are various LFA formats, biorecognition molecules, labels, detection systems, and applications. − LFAs have been used to monitor infectious agents, ,, nucleic acids, , proteins, cells, veterinary drugs, , toxins, − and pesticides . The most popular test to date is the well-known and well-received pregnancy test.…”

mentioning

confidence: 99%

“…There are various LFA formats, biorecognition molecules, labels, detection systems, and applications. 4−7 LFAs have been used to monitor infectious agents, 5,8,9 nucleic acids, 10,11 proteins, 12 cells, 13 veterinary drugs, 14,15 toxins, 16−18 and pesticides. 19 The most popular test to date is the well-known and well-received pregnancy test.…”

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confidence: 99%

Miniaturized Flow Stacked Immunoassay for Detecting Escherichia coli in a Single Step

Eltzov

Marks

2016

Anal. Chem.

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Commercially available systems that provide cost-effective, fast, simple, and portable solutions for health and environmental applications are few despite advancements in bioassays and biosensor research. We have developed a new system based on stacked membranes, each layer with a specific function. Samples were added onto the bottom-most layer, and as each layer becomes wet, the analyte pushes through to the next membrane layers. During migration, the analyte attaches with the corresponding antibody, itself conjugated with horseradish peroxidase (HRP) to produce a measurable signal. To prevent false positive results, blocking layer membranes are added to stop unbound antibodies from reaching the top membrane. Thus, only analyte/antibody-HRP complex will generate a signal. In order to prove this concept, Escherichia coli was used as the target analyte. After optimization, our immunoassay sensitivity was adjusted to 100 cells mL(-1). Different environmental water sources were also tested to demonstrate the sensitivity and specificity of our proposed stacked bioassay. Simplicity, low price, sensitivity, and modularity (capability to change to any target analyte) make this idea very promising for future commercialization.

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“…In a previous study, anti-HES polyclonal antibody was prepared and applied in a one-step membrane-based competitive colloidal gold-based immunoassay in lateral flow format for the determination of HES in swine urine and liver [ 37 ]. However, the sensitivity of the test strip was far higher than 30 µg kg −1 by unaided visual assessment, which is much higher than ours, suggesting the strip developed in this study is more sensitive.…”

Section: Resultsmentioning

confidence: 99%

A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

et al. 2018

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A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg−1, and the limit of detection value was 0.62 µg kg−1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.

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Immumochromatographic assay for determination of hexoestrol residues

Cited by 5 publications

References 20 publications

Gold nanoparticles in biomedical applications: recent advances and perspectives

Gold nanoparticles in biomedical applications: recent advances and perspectives

Miniaturized Flow Stacked Immunoassay for Detecting Escherichia coli in a Single Step

A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

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