Immune activation with peptide assemblies carrying Lewis y tumor‐associated carbohydrate antigen

Yamazaki, Y.; Watabe, Naoki; Obata, Hiroaki; Hara, Eri; Ohmae, Masashi; Kimura, Shunsaku

doi:10.1002/psc.2926

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…In addition, Yamazaki and co-workers also investigated immune activation with polypeptide assemblies carrying Lewis y (Figure ). Carbohydrate antigen Lewis y was conjugated to poly(sarcosine) n - b -poly( l -lactic acid) 30 ( m = 15 or 50) and poly(sarcosine) m - b -( d / l -Leu-Aib) n ( m = 22 or 30, n = 6 or 8). It was shown that the presentation and the antigenicity of Lewis y are largely dependent on the morphologies of the molecular assemblies.…”

Section: Immunologymentioning

confidence: 95%

“…In addition, Yamazaki and co-workers also investigated immune activation with polypeptide assemblies carrying Lewis y (Figure 72). 572 It was shown that the presentation and the antigenicity of Lewis y are largely dependent on the morphologies of the molecular assemblies. Among the various morphologies, including nanosheet, curved sheet, nanotube, and vesicle, a nanosheet possessing the highest density of Lewis y displayed the best effect to produce anti-Lewis y IgM.…”

Section: Carbohydrate-based Vaccine and Immunotherapymentioning

confidence: 99%

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Carbohydrate-Based Macromolecular Biomaterials

et al. 2021

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Carbohydrates are the most abundant and one of the most important biomacromolecules in Nature. Except for energy-related compounds, carbohydrates can be roughly divided into two categories: Carbohydrates as matter and carbohydrates as information. As matter, carbohydrates are abundantly present in the extracellular matrix of animals and cell walls of various plants, bacteria, fungi, etc., serving as scaffolds. Some commonly found polysaccharides are featured as biocompatible materials with controllable rigidity and functionality, forming polymeric biomaterials which are widely used in drug delivery, tissue engineering, etc. As information, carbohydrates are usually referred to the glycans from glycoproteins, glycolipids, and proteoglycans, which bind to proteins or other carbohydrates, thereby meditating the cell–cell and cell–matrix interactions. These glycans could be simplified as synthetic glycopolymers, glycolipids, and glycoproteins, which could be afforded through polymerization, multistep synthesis, or a semisynthetic strategy. The information role of carbohydrates can be demonstrated not only as targeting reagents but also as immune antigens and adjuvants. The latter are also included in this review as they are always in a macromolecular formulation. In this review, we intend to provide a relatively comprehensive summary of carbohydrate-based macromolecular biomaterials since 2010 while emphasizing the fundamental understanding to guide the rational design of biomaterials. Carbohydrate-based macromolecules on the basis of their resources and chemical structures will be discussed, including naturally occurring polysaccharides, naturally derived synthetic polysaccharides, glycopolymers/glycodendrimers, supramolecular glycopolymers, and synthetic glycolipids/glycoproteins. Multiscale structure–function relationships in several major application areas, including delivery systems, tissue engineering, and immunology, will be detailed. We hope this review will provide valuable information for the development of carbohydrate-based macromolecular biomaterials and build a bridge between the carbohydrates as matter and the carbohydrates as information to promote new biomaterial design in the near future.

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Section: Immunologymentioning

confidence: 95%

Section: Carbohydrate-based Vaccine and Immunotherapymentioning

confidence: 99%

Carbohydrate-Based Macromolecular Biomaterials

et al. 2021

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“…[30] On the contrary, nanoparticles were used as a vehicle for a tumor-associated carbohydrate antigen (TACA) to trigger the immune response. [31] In this case, nanoparticles displayed TACA as well as poly(sarcosine) on the surface, but antigenicity of poly(sarcosine) could be lowered apparently by making the surface density of poly(sarcosine) as high as polymer brush. As a result, TACA becomes the main component on the surface left behind as an antigen to promote the antibody production.…”

Section: Introductionmentioning

confidence: 90%

“…A biological design was also examined to use sialic acid, which will induce B cell apoptosis upon binding to Siglec‐G and CD22 which are expressed on mouse B cell. On the contrary, nanoparticles were used as a vehicle for a tumor‐associated carbohydrate antigen (TACA) to trigger the immune response . In this case, nanoparticles displayed TACA as well as poly(sarcosine) on the surface, but antigenicity of poly(sarcosine) could be lowered apparently by making the surface density of poly(sarcosine) as high as polymer brush.…”

Section: Introductionmentioning

confidence: 99%

Modulation of immunogenicity of poly(sarcosine) displayed on various nanoparticle surfaces due to different physical properties

Kim

Hara

Watabe

et al. 2017

Journal of Peptide Science

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Poly(sarcosine) displayed on polymeric micelle is reported to trigger a T cell-independent type2 reaction with B1a cells in the mice to produce IgM and IgG3 antibodies. In addition to polymeric micelle, three kinds of vesicles displaying poly(sarcosine) on surface were prepared here to evaluate the amounts and avidities of IgM and IgG3, which were produced in mice, to correlate them with physical properties of the molecular assemblies. The largest amount of IgM was produced after twice administrations of a polymeric micelle of 35 nm diameter (G1). On the other hand, the production amount of IgG3 became the largest after twice administrations of G3 (vesicle of 229 nm diameter) or G4 (vesicle of 85 nm diameter). The augmented avidity of IgG3 after the twice administrations compared with that at the single administration was the highest with G3. These differences in immune responses are discussed in terms of surface density of poly(sarcosine) chains, nanoparticle size, hydrophobic component of poly(L-lactic acid) or (Leu- or Val-Aib) , and membrane elasticity of the nanoparticles. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.

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“…The thickness was about 10 nm, indicating the interdigitated monolayer structure comprising the heterochiral helical peptides. The surface density of poly(sarcosine) on the nanosheet was so high that immune cells could not recognize a possible antigen of poly(sarcosine) chains covering the nanosheet. , Furthermore, the surfaces of molecular assemblies prepared from SL and SD were able to be functionalized with thiol, , adenine, and oligosaccharide , by their introductions at the terminal of poly(sarcosine) chains. Accordingly, the peptide nanosheets can be designed to have two faces, one for the phenylboronic acid to connect to antibodies and the other for the photoactivatable radical generator to react with PP fiber.…”

Section: Introductionmentioning

confidence: 99%

A Novel Surface Modification and Immobilization Method of Anti-CD25 Antibody on Nonwoven Fabric Filter Removing Regulatory T Cells Selectively

et al. 2019

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Anti-CD25 antibodies were immobilized on polypropylene (PP) nonwoven fabrics to specifically remove mouse regulatory T cells (Tregs) from mouse spleen cells. PP fibers were coated with peptide nanosheets, which were prepared by self-assembling of a mixture of X-poly(sarcosine)-b-(l-Leu-Aib)6 (X: glycolic acid or a phenylboronic acid) and Y-poly(sarcosine)-b-(d-Leu-Aib)6 (Y: glycolic acid or diazirine derivative). Anti-CD25 antibodies were immobilized by covalent linking between the sugar moiety of the antibody and the phenylboronic acid group on the peptide nanosheet. The removal rate of mouse Tregs from the mouse spleen cells was more than 95% only by passing the filters, while the nonspecific removal rates of other cells were less than 15%. The coating of peptide nanosheets on PP fibers was very effective to provide a suitable environment for the immobilized antibody to interact with the counterpart cells while the coating suppressed nonspecific adsorption of other cells.

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Immune activation with peptide assemblies carrying Lewis y tumor‐associated carbohydrate antigen

Cited by 7 publications

References 26 publications

Carbohydrate-Based Macromolecular Biomaterials

Carbohydrate-Based Macromolecular Biomaterials

Modulation of immunogenicity of poly(sarcosine) displayed on various nanoparticle surfaces due to different physical properties

A Novel Surface Modification and Immobilization Method of Anti-CD25 Antibody on Nonwoven Fabric Filter Removing Regulatory T Cells Selectively

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