Immune and non-immune T4 radioassays utilizing albumin magnetic microparticles

Ithakissios, Dionyssis S.; Kubiatowicz, D O; Windorski, D C; Wicks, Joan

doi:10.1016/0009-8981(78)90478-3

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…We made sure that our extraction was successful1 by adding T4-5-125 and T3-J-125 as tracer substances. This procedure seems to be much more suitable than controlling the success of the extraction by recovery in the RIA (BORNER, 1979;ITHAKISSIOS, 1978), because this method is independent of the testkit's sensitivity. So we could demonstrate that the charcoal extraction did not yield better results than about 93 % with human serum, even after four extractions.…”

Section: Discussionmentioning

confidence: 99%

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Serum Effects on Thyroxine and Triiodothyronine Levels

Hanauer

Schroth

1987

Journal of Veterinary Medicine Series A

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The aim of the investigation was to study the influence of serum of different species on commercial radioimmunoassays for T 4 and T 3. Therefore a largely T 4-and T 3-free serum or plasma of man, rat, pig, sheep, cattle, horse, dog, and chicken was produced. The standard method of extracting T 4 and T 3 from serum or plasma with charcoal led only to extraction results of 93 %. This could be improved to 99.5 % or more by using a batch-technique with a resin. The success of the extraction was controlled by labelled hormones in both cases.The largely T4-and T3-free sera or plasma were used in radioimmunoassays of various manufacturers. The results were influenced by the different species, indicating that the serum composition and the different binding fractions for thyroid hormones play a large part in the values obtained. This could be confirmed by a serum dilution with Tyrode's solution in the ratio of 1 : 1, which yielded false negative values in all cases investigated.The investigation showed that radioimmunoassays developed for human medicine cannot be used without adaptation to the special sera or plasma. Also a RIA designed for the dog could not be used for all species in veterinary medicine. The hormone values measured with unadapted testkits should therefore be doubted.

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Section: Discussionmentioning

confidence: 99%

“…The standard method for removing T 4 and T 3 from serum is the extraction with charcoal, described by others (BORNER, 1979;CHEUNG and RILEY, 1976;HOLLANDER and SHANKMAN, 1974;ITHAKISSIOS et al, 1978;LARSEN and FRUMESS, 1977;MITSUMA et al, 1972;NYE et al, 1976). In this study 1 .O g of charcoal (Nr.…”

Section: Extraction With Charcoalmentioning

confidence: 97%