Immune and oxidative stress disorder in ovulation-dysfunction women revealed by single-cell transcriptome

Abstract

IntroductionOvulation dysfunction is now a widespread cause of infertility around the world. Although the impact of immune cells in human reproduction has been widely investigated, systematic understanding of the changes of the immune atlas under female ovulation remain less understood.MethodsHere, we generated single cell transcriptomic profiles of 80,689 PBMCs in three representative statuses of ovulation dysfunction, i.e., polycystic ovary syndrome (PCOS), primary ovarian insufficiency (POI) and menopause (MENO), and identified totally 7 major cell types and 25 subsets of cells.ResultsOur study revealed distinct cluster distributions of immune cells among individuals of ovulation disorders and health. In patients with ovulation dysfunction, we observed a significant reduction in populations of naïve CD8 T cells and effector memory CD4 T cells, whereas circulating NK cells and regulatory NK cells increased.DiscussionOur results highlight the significant contribution of cDC-mediated signaling pathways to the overall inflammatory response within ovulation disorders. Furthermore, our data demonstrated a significant upregulation of oxidative stress in patients with ovulation disorder. Overall, our study gave a deeper insight into the mechanism of PCOS, POI, and menopause, which may contribute to the better diagnosis and treatments of these ovulatory disorder.

Juvenile idiopathic arthritis and primary ovarian failure: a two-sample Mendelian randomization analysis in a mixed-gender cohort

“…Furthermore, the compromised killing activity of NK cells may contribute to extensive activation and expansion of cytotoxic CD8 T lymphocytes, production of IFNγ and other macrophage activating factors, which in turn stimulate macrophages for prolonged periods, resulting in excessive activation and expansion of macrophages that induce the onset and progression of JIA through heightened inflammatory immune activation ( 28 ). Moreover, another study observed abnormal changes in NK cells following the same single-cell data analysis of POF ( 29 ); it can be inferred that these abnormal changes in NK cells predispose individuals to long-term inflammation, potentially contributing to ovarian dysfunction and diminished ovarian reserve.…”