Immune Cell Plasticity in Inflammation: Insights into Description and Regulation of Immune Cell Phenotypes

Margraf, Andreas; Perretti, Mauro

doi:10.3390/cells11111824

Cited by 20 publications

(18 citation statements)

References 291 publications

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“…This indicated that a smaller entry door size did not pose a significant barrier to the cell infiltration, which can be due to the high plasticity of immune cells. [ 19 ] Macrophages and FcεRI+ cells (such as Langerhan cells [LC], monocytes, mast cells, and other dendritic cells [DC]) dominated the immune cell infiltration during the implantation [ 20 ] (Figure 2c). The immune cell recruitment followed a size‐dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds

Liu,

Suarez‐Arnedo,

Caston

et al. 2023

Advanced Materials

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Microporous annealed particle (MAP) scaffolds are injectable granular materials comprised of micron sized hydrogel particles (microgels). The diameter of these microgels directly determines the size of the interconnected void space between particles where infiltrating or encapsulated cells reside. This tunable porosity allows us to use MAP scaffolds to study the impact of spatial confinement (SC) on both cellular behaviors and the host response to biomaterials. Despite previous studies showing that pore size and SC influence cellular phenotypes, including mitigating the macrophage inflammatory response, there is still a gap in knowledge regarding how SC within a biomaterial modulates immune cell recruitment in vivo in wounds and implants. Thus, we studied the immune cell profile within confined and unconfined biomaterials using small (40 μm), medium (70 μm), and large (130 μm) diameter spherical microgels, respectively. We discovered that MAP scaffolds impart regenerative wound healing with an IgG1‐biased Th2 response. MAP scaffolds made with large microgels promoted a balanced pro‐regenerative macrophage response, resulting in enhanced wound healing with mature collagen regeneration and reduced inflammation levels.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds

Liu,

Suarez‐Arnedo,

Caston

et al. 2023

Advanced Materials

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“…Activation of peripheral immune cells can induce two different phenotypes – anti-inflammatory or pro-inflammatory states – depending on stimulator patterns [40]. The classic pro-inflammatory phenotype is characterised by activation of the NF-κB pathway, and increased production and release of the proinflammatory cytokines TNF, IL-1 and IL-6 [41, 42]. Although the increase in PBMCs NF-κB levels were not significant in this study, ACTH-treated animals had increased serum levels of IL-6 when compared to saline-treated controls, demonstrating an increase in proinflammatory signalling in the present model.…”

Section: Discussionmentioning

confidence: 99%

The role of IL-6 in dopamine dysregulation underlying anhedonia phenotype in rats

Varela,

Macpherson,

Houghton

et al. 2023

Preprint

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AimsTo investigate the role of peripheral metabolic change and chronic low-grade inflammation on striatal dopamine dynamics and anhedonia-like behaviour induced by hypothalamic–pituitary–adrenal (HPA) axis disruption.MethodsWistar rats were trained in a progressive-ratio/concurrent effort-related choice paradigm to assess effort-related decision making. After reaching a stable baseline, animals received daily injections of adrenocorticotrophic hormone (ACTH) or saline for 24 days. On the 23rdand 24thday, animals received a bupropion challenge (10mg/kg and 20mg/kg respectively) 30 minutes prior to the behavioural testing session. On the 25thday, animals received a single injection of bupropion (20mg/kg) 30 minutes prior to euthanasia. Peripheral and central inflammatory markers were assessed through ELISA and In-Cell Western assay; glucose transport activity was assessed in peripheral blood mononuclear cells though a commercial assay kit; brain levels of dopaminergic and inflammatory markers were assessed in the nucleus accumbens (NAc) and prefrontal cortex (PFC) through immunohistochemistry; and serum central carbon metabolism metabolites were assessed through a metabolomics approach.ResultsACTH induced an anhedonia-like phenotype, decreased tyrosine hydroxylase (TH) levels in the NAc, increased peripheral IL-6 levels, and decreased glucose transport activity and glucose metabolites when compared to control group. Bupropion treatment was not able to reverse the anhedonic phenotype. Glucose uptake was positively correlated to behaviour; TH levels were correlated to microglia volume; metabolites were correlated to TH levels; and IL6 was correlated to TH levels and metabolites.ConclusionChronic ACTH treatment can induce treatment-resistant anhedonia in rats, and the interaction between peripheral immunometabolic state and central dopamine synthesis is a potential mechanism underlying this phenotype.

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“…Macrophage efferocytosis of apoptotic PMNs is an essential cellular response to enhance the clearance of infection and the resolution of inflammation. 38,39 Pro-resolving mediators are potent bioactive agonists and regulators of macrophage efferocytosis of apoptotic neutrophils in both human cells and murine models of inflammation. 39 We set out to determine if 4S,5R-RCTR1 enhances M2-like macro-…”

Section: Synthetic 4s5r-rctr1 Increases Efferocytosis Of Apoptotic Ne...mentioning

confidence: 99%

Stereochemistry and functions of the new cysteinyl‐resolvin, 4S,5R‐RCTR1, in efferocytosis and erythrophagocytosis of human senescent erythrocytes

et al. 2023

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Specialized pro‐resolving lipid mediators play key functions in the resolution of the acute inflammatory response. Herein, we elucidate the stereochemical structure of the new 4S,5R‐RCTR1, a cysteinyl‐resolvin, recently uncovered in human leukocytes incubated with a 4S,5S‐epoxy‐resolvin intermediate, using liquid chromatography–tandem mass spectrometry (LC–MS/MS) and ultra‐violet (UV) spectrophotometry. With this approach, the physical properties of the new mediator prepared by total organic synthesis were matched to enzymatically produced biogenic material. In addition, we confirmed the potent biological actions of 4S,5R‐RCTR1 with human M2‐like macrophage phagocytosis of live bacteria, efferocytosis of apoptotic neutrophils, and erythrophagocytosis of senescent human red blood cells in a concentration‐dependent manner from 0.1 to 10 nM. Taken together, these results establish the complete stereochemistry of 4S,5R‐RCTR1 as 5R‐glutathionyl‐4S,17S‐dihydroxy‐6E,8E,10Z,13Z,15E,19Z‐docosahexaenoic acid and give evidence of its novel bioactivities in human phagocyte responses. Moreover, they confirm and extend the stereoselective functions of the 4S,5R‐RCTR1 with isolated human phagocytes of interest in the resolution of inflammation.

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Immune Cell Plasticity in Inflammation: Insights into Description and Regulation of Immune Cell Phenotypes

Cited by 20 publications

References 291 publications

Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds

Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds

The role of IL-6 in dopamine dysregulation underlying anhedonia phenotype in rats

Stereochemistry and functions of the new cysteinyl‐resolvin, 4S,5R‐RCTR1, in efferocytosis and erythrophagocytosis of human senescent erythrocytes

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